Ticlopidine hydrochloride

Name: Ticlopidine hydrochloride
Brand: MedChemExpress
SKU: HY-B0153A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0153A Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ticlopidine hydrochloride is an adenosine diphosphate (ADP) receptor inhibitor against platelet aggregation with IC50 of ~2 μM.

For research use only. We do not sell to patients.

Ticlopidine hydrochloride Chemical Structure

CAS No. : 53885-35-1

Size	Price	Stock	Quantity
1 g	USD 35	In-stock	Estimated Time of Arrival: December 31
5 g	USD 95	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ticlopidine hydrochloride:

Ticlopidine-d₄ hydrochloride Get quote
Ticlopidine Get quote
Ticlopidine hydrochloride (Standard) Get quote

1 Publications Citing Use of MCE Ticlopidine hydrochloride

•Biochem Biophys Res Commun. 2024 Jul 23:733:150436. [Abstract]

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Adenosine A₁ receptor (A₁R) Adenosine A_2A receptor (A_2AR) Adenosine A_2B receptor (A_2BR) Adenosine A₃ receptor (A₃R)

Biological Activity
Purity & Documentation
References
Customer Review

Description

Ticlopidine hydrochloride is an adenosine diphosphate (ADP) receptor inhibitor against platelet aggregation with IC50 of ~2 μM. Target: Adenosine diphosphate (ADP) Ticlopidine (trade name Ticlid) is an antiplatelet drug in the thienopyridine family. Ticlopidine hydrochloride inhibits platelet aggregation with IC50 of ~2 μM in men. Like clopidogrel, it is an adenosine diphosphate (ADP) receptor inhibitor. It is used in patients in whom aspirin is not tolerated, or in whom dual antiplatelet therapy is desirable. Because it has been reported to increase the risk of thrombotic thrombocytopenic purpura (TTP) and neutropenia, its use has largely been supplanted by the newer drug, clopidogrel, which is felt to have a much lower hematologic risk. Its niche role as an alternative in those patients who do not tolerate Clopidogrel has now been superdeded by Ticagrelor and Prasugrel. The usual dose is 250 mg twice daily by the oral route. Ticlopidine hydrochloride, when orally administered to rats, results in activation of basal and prostaglandin E1 (PGE1)-stimulated adenylate cylase activity through increase in affinity of the cyclase in platelet membrane to PGE1, although it failed to affect adenosine- or sodium fluoride-stimulated activity of the enzyme.

Cellular Effect

Cell Line	Type	Value	Description	References
Platelet	IC₅₀	0.36 mM Compound: Ticlid	Antiplatelet aggregation activity in rabbit platelets assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins prior to ADP-challenge measured within 5 mins by Born's turbidimetric analysis Antiplatelet aggregation activity in rabbit platelets assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins prior to ADP-challenge measured within 5 mins by Born's turbidimetric analysis	[PMID: 23509954]

Clinical Trial

Molecular Weight

300.25

Formula

C₁₄H₁₅Cl₂NS

CAS No.

53885-35-1

Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC=CC=C1CN2CCC3=C(C=CS3)C2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (166.53 mM; Need ultrasonic)

DMSO : 50 mg/mL (166.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3306 mL	16.6528 mL	33.3056 mL
5 mM	0.6661 mL	3.3306 mL	6.6611 mL
10 mM	0.3331 mL	1.6653 mL	3.3306 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (27.74 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (250 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Thebault JJ, et al. Effects of ticlopidine, a new platelet aggregation inhibitor in man. Clin Pharmacol Ther. 1975 Oct;18(4):485-90. [Content Brief]

[2]. Ashida SI, et al. Mode of action of ticlopidine in inhibition of platelet aggregation in the rat. Thromb Haemost. 1979 Apr 23;41(2):436-49. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.3306 mL	16.6528 mL	33.3056 mL	83.2639 mL
	5 mM	0.6661 mL	3.3306 mL	6.6611 mL	16.6528 mL
	10 mM	0.3331 mL	1.6653 mL	3.3306 mL	8.3264 mL
	15 mM	0.2220 mL	1.1102 mL	2.2204 mL	5.5509 mL
	20 mM	0.1665 mL	0.8326 mL	1.6653 mL	4.1632 mL
	25 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3306 mL
	30 mM	0.1110 mL	0.5551 mL	1.1102 mL	2.7755 mL
	40 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0816 mL
	50 mM	0.0666 mL	0.3331 mL	0.6661 mL	1.6653 mL
	60 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	80 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
	100 mM	0.0333 mL	0.1665 mL	0.3331 mL	0.8326 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.