Trichlormethiazide

Cat. No.: HY-B0235 Purity: 98.59%: FAQs
Data Sheet SDS COA Handling Instructions

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Trichlormethiazide is an orally active thiazide diuretic, with antihypertensive effect. Trichlormethiazide increases urine volume (UV), Na and K excretion and tends to improve the depressed creatinine clearance (CCRE) in acute renal failure rats model.

For research use only. We do not sell to patients.

Trichlormethiazide Chemical Structure

CAS No. : 133-67-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
5 g	USD 100	In-stock	Estimated Time of Arrival: December 31
10 g	USD 160	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Other Forms of Trichlormethiazide:

Trichlormethiazide sodium Get quote
Trichlormethiazide (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vivo

Trichlormethiazide (1 mg/kg; p.o.; once) increases urinary volume, sodium and potassium excretion in rats^[1].
Trichlormethiazide (10 mg/kg, i.v.; daily for 5 days) significantly reduces mean arterial pressure (MAP) within 24 h in high salt intake (HS) rats receiving angiotensin II, but does not affect MAP in any other group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats, weighing 170-300 g^[1]
Dosage:	1 mg/kg
Administration:	Oral administration, once
Result:	Significantly increased potassium excretion in normal rats. Significantly increased urinary volume, sodium and potassium excretion in cisplatin-induced ARF (acute renal failures) rats.

Animal Model:	Male Sprague-Dawley rats (350-450 g)^[2]
Dosage:	10 mg/kg
Administration:	Intravenous injection, daily, for 15 days
Result:	Produced a significant fall in MAP in rats on combined angiotensin II and high salt intake.

Molecular Weight

380.66

Formula

C₈H₈Cl₃N₃O₄S₂

CAS No.

133-67-5

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=C(Cl)C=C(C2=C1)NC(C(Cl)Cl)NS2(=O)=O)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (394.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6270 mL	13.1351 mL	26.2702 mL
5 mM	0.5254 mL	2.6270 mL	5.2540 mL
10 mM	0.2627 mL	1.3135 mL	2.6270 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.59%

Select Batch:

Data Sheet (277 KB) SDS (418 KB)

COA (251 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. K Yao, et al. Diuretic effects of KW-3902, a novel adenosine A1-receptor antagonist, in various models of acute renal failure in rats. Jpn J Pharmacol. 1994 Apr;64(4):281-8. [Content Brief]

[2]. J R Ballew, et al. Characterization of the antihypertensive effect of a thiazide diuretic in angiotensin II-induced hypertension. J Hypertens. 2001 Sep;19(9):1601-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6270 mL	13.1351 mL	26.2702 mL	65.6754 mL
	5 mM	0.5254 mL	2.6270 mL	5.2540 mL	13.1351 mL
	10 mM	0.2627 mL	1.3135 mL	2.6270 mL	6.5675 mL
	15 mM	0.1751 mL	0.8757 mL	1.7513 mL	4.3784 mL
	20 mM	0.1314 mL	0.6568 mL	1.3135 mL	3.2838 mL
	25 mM	0.1051 mL	0.5254 mL	1.0508 mL	2.6270 mL
	30 mM	0.0876 mL	0.4378 mL	0.8757 mL	2.1892 mL
	40 mM	0.0657 mL	0.3284 mL	0.6568 mL	1.6419 mL
	50 mM	0.0525 mL	0.2627 mL	0.5254 mL	1.3135 mL
	60 mM	0.0438 mL	0.2189 mL	0.4378 mL	1.0946 mL
	80 mM	0.0328 mL	0.1642 mL	0.3284 mL	0.8209 mL
	100 mM	0.0263 mL	0.1314 mL	0.2627 mL	0.6568 mL