Tryptamine (Synonyms: 3-(2-Aminoethyl)indole~2-(3-Indolyl)ethylamine)

Name: Tryptamine
Brand: MedChemExpress
SKU: HY-B2132
Rating: 4.5 (1 reviews)

Cat. No.: HY-B2132 Purity: 98.04%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Tryptamine is a selective, blood-brain-penetrating 5-HT4 receptor agonist (EC50=1-3 mM) and an endogenous ligand of the aryl hydrocarbon receptor (AHR) (Kd=10-50 nM). Tryptamine promotes intestinal anion secretion and fluid transport by activating G protein-coupled receptors (GPCRs) and accelerates gastrointestinal motility. Tryptamine regulates Th17/Treg balance to inhibit neuroinflammation, competitively binds to 5-HT receptors to regulate central nervous system activity, and participates in temperature regulation and spinal reflex regulation as a neuromodulator. Tryptamine can be used to study intestinal motility disorders such as functional constipation, and has shown significant efficacy in multiple sclerosis models.

For research use only. We do not sell to patients.

Tryptamine Chemical Structure

CAS No. : 61-54-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tryptamine:

1 Publications Citing Use of MCE Tryptamine

•Int J Mol Sci. 2022, 23(20), 12420.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	> 50 μM Compound: Tryptamine	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability by cell titer 96 aqueous one solution based assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability by cell titer 96 aqueous one solution based assay	[PMID: 29057053]
MDA-MB-231	IC₅₀	> 50 μM Compound: Tryptamine	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability by cell titer 96 aqueous one solution based assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability by cell titer 96 aqueous one solution based assay	[PMID: 29057053]

In Vitro

Tryptamine (100 μM; 48 h) significantly inhibits MOG35-55-specific CD4+ T cell proliferation and RORγT+ cell differentiation in T cell proliferation assay^[2].

Tryptamine (1 mM; 1 h) significantly increases intracellular cAMP levels in colon organoid cAMP assay, revealing activation of 5-HT4R signaling pathway^[3].
Tryptamine (1 mM; 30 min) significantly increases the surface area of ??colon organoids from GF and HM mice in colon organoid swelling assay, demonstrating promotion of fluid secretion^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	Mouse splenic CD4+ T cells (MOG35-55 immunized)
Concentration:	100 μM
Incubation Time:	48 h
Result:	Reduced PCNA+ proliferating CD4+ T cells by 40-50% and decreased RORγT+ cell population by 35-40% compared to vehicle. Increased FoxP3+ Treg frequency by 20-25%.

In Vivo

Tryptamine (0.5 mg/kg, 12 mg/kg; intravenous injection; single dose) induces elevated body temperature and enhances spinal reflex activity in rabbit models^[1].
Tryptamine (12.5 mg/kg; intraperitoneal injection; once every 2 days; 13 days) significantly alleviates paralysis symptoms, reduces CD4+ T cell infiltration in the central nervous system and promotes Treg differentiation in a chronic progressive EAE model in C57BL/6 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rabbits^[1]
Dosage:	.5 mg/kg, 12 mg/kg
Administration:	Single intravenous injection
Result:	Induced dose-dependent hyperthermia (1-3°C increase) and enhanced spinal reflex amplitudes by 50-80% in both species. Observed behavioral excitation including clonic convulsions.

Animal Model:	C57BL/6 mice (female, 6-8 weeks old) with chronic progressive EAE^[2]
Dosage:	12.5 mg/kg (sterile corn oil with 2% DMSO)
Administration:	Intraperitoneal injection every 48 h from day 1 to day 13
Result:	Reduced clinical paralysis scores by 40-50% compared to vehicle control. CNS infiltration of CD4+ T cells decreased by 30-40%, while increased splenic FoxP3+ Tregs by 50-60%. IL-17 secretion was suppressed by 60-70% in CNS mononuclear cells.

Animal Model:	Germ-free (GF) Swiss Webster mice (male, 8-12 weeks old) colonized with engineered B. thetaiotaomicron TrpD+^[3]
Dosage:	Oral gavage with 300 μL bacterial culture (single inoculation), 0.25% tryptophan in drinking water for 5 days
Administration:
Result:	Increased fecal tryptamine concentration 2-3 fold compared to control strain. Reduced whole gut transit time by 35-40%, and increased fecal water content by 20-25%.

Molecular Weight

160.22

Formula

C₁₀H₁₂N₂

CAS No.

61-54-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

NCCC1=CNC2=C1C=CC=C2

Structure Classification

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 150 mg/mL (936.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.2414 mL	31.2071 mL	62.4142 mL
5 mM	1.2483 mL	6.2414 mL	12.4828 mL
10 mM	0.6241 mL	3.1207 mL	6.2414 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (282 KB) SDS (615 KB)

COA (250 KB) HNMR (221 KB) RP-HPLC (220 KB)

Handling Instructions (2659 KB)

References

[1]. Jones RS, et al. Tryptamine: a neuromodulator or neurotransmitter in mammalian brain? Prog Neurobiol. 1982;19(1-2):117-39. [Content Brief]

[2]. Dopkins N, et al. Tryptamine Attenuates Experimental Multiple Sclerosis Through Activation of Aryl Hydrocarbon Receptor. Front Pharmacol. 2021 Jan 25;11:619265. [Content Brief]

[3]. Bhattarai Y, et al. Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. Cell Host Microbe. 2018 Jun 13;23(6):775-785.e5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.2414 mL	31.2071 mL	62.4142 mL	156.0355 mL
	5 mM	1.2483 mL	6.2414 mL	12.4828 mL	31.2071 mL
	10 mM	0.6241 mL	3.1207 mL	6.2414 mL	15.6035 mL
	15 mM	0.4161 mL	2.0805 mL	4.1609 mL	10.4024 mL
	20 mM	0.3121 mL	1.5604 mL	3.1207 mL	7.8018 mL
	25 mM	0.2497 mL	1.2483 mL	2.4966 mL	6.2414 mL
	30 mM	0.2080 mL	1.0402 mL	2.0805 mL	5.2012 mL
	40 mM	0.1560 mL	0.7802 mL	1.5604 mL	3.9009 mL
	50 mM	0.1248 mL	0.6241 mL	1.2483 mL	3.1207 mL
	60 mM	0.1040 mL	0.5201 mL	1.0402 mL	2.6006 mL
	80 mM	0.0780 mL	0.3901 mL	0.7802 mL	1.9504 mL
	100 mM	0.0624 mL	0.3121 mL	0.6241 mL	1.5604 mL

Tryptamine Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tryptamine61-54-13-(2-Aminoethyl)indole~2-(3-Indolyl)ethylamineEndogenous Metabolite5-HT ReceptorAryl Hydrocarbon ReceptorSerotonin Receptor5-hydroxytryptamine ReceptorAhRInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Tryptamine (Synonyms: 3-(2-Aminoethyl)indole~2-(3-Indolyl)ethylamine)

Customer Review

Other Forms of Tryptamine:

Tryptamine Related Antibodies

1 Publications Citing Use of MCE Tryptamine

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Tryptamine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tryptamine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report