Z-321

Name: Z-321
Brand: MedChemExpress
SKU: HY-19123
Rating: 4.5 (1 reviews)

Cat. No.: HY-19123 Purity: 99.72%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Z-321 is a prolylendopeptidase (PEP) inhibitor.

For research use only. We do not sell to patients.

Z-321 Chemical Structure

CAS No. : 130849-58-0

Size	Price	Stock	Quantity
5 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Z-321 is a prolylendopeptidase (PEP) inhibitor.

IC₅₀ & Target

PEP^[1]

In Vivo

Z-321 is a prolylendopeptidase (PEP) inhibitor. In the 100 or 200 mg/kg Z-321-treated groups, mean lordosis quotient (LQ) decreases after administration. However, there is no statistical difference among the values before and after. In contrast, the mean LQ in the 300 mg/kg Z-321-treated females is lower than that before injection (P<0.005). Furthermore, when compare to that in the vehicle-treated control group, mean LQ is also significantly lower (p<0.05). The incidence of soliciting behavior decreases after treatment with 300 mg/kg Z-321, when compare to that before treatment, but there is no statistical difference. The present study also demonstrates that 300 mg/kg Z-321 is effective in inhibiting lordosis behavior without disturbance of locomotor activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

344.47

Formula

C₁₉H₂₄N₂O₂S

CAS No.

130849-58-0

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1N(C(CC2CC3=C(C=CC=C3)C2)=O)CSC1)N4CCCC4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (725.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9030 mL	14.5151 mL	29.0301 mL
5 mM	0.5806 mL	2.9030 mL	5.8060 mL
10 mM	0.2903 mL	1.4515 mL	2.9030 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (18.14 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (18.14 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 6.25 mg/mL (18.14 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (253 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. Oosuka I, et al. Decrease of sexual receptivity by prolylendopeptidase inhibitor in female rats. Jpn J Pharmacol. 2000 May;83(1):82-5. [Content Brief]

Animal Administration
^[1]

Femal wistar rats (200 to 250 g) are used and housed under conditions of controlled temperature (23 to 26°C) and photoperiod (14: 10 h, light:dark). Fifty four rats are ovariectomized under ether anesthesia. Two weeks after ovariectomy, sexual behavioral tests are carried out before and after a treatment with Z-321 (100, 200 or 300 mg/kg). Another group of rats, 7 females are given 1 mL/kg of 10% gum arabic as control group^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Oosuka I, et al. Decrease of sexual receptivity by prolylendopeptidase inhibitor in female rats. Jpn J Pharmacol. 2000 May;83(1):82-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9030 mL	14.5151 mL	29.0301 mL	72.5753 mL
	5 mM	0.5806 mL	2.9030 mL	5.8060 mL	14.5151 mL
	10 mM	0.2903 mL	1.4515 mL	2.9030 mL	7.2575 mL
	15 mM	0.1935 mL	0.9677 mL	1.9353 mL	4.8384 mL
	20 mM	0.1452 mL	0.7258 mL	1.4515 mL	3.6288 mL
	25 mM	0.1161 mL	0.5806 mL	1.1612 mL	2.9030 mL
	30 mM	0.0968 mL	0.4838 mL	0.9677 mL	2.4192 mL
	40 mM	0.0726 mL	0.3629 mL	0.7258 mL	1.8144 mL
	50 mM	0.0581 mL	0.2903 mL	0.5806 mL	1.4515 mL
	60 mM	0.0484 mL	0.2419 mL	0.4838 mL	1.2096 mL
	80 mM	0.0363 mL	0.1814 mL	0.3629 mL	0.9072 mL
	100 mM	0.0290 mL	0.1452 mL	0.2903 mL	0.7258 mL

Z-321 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-321130849-58-0Z321Z 321OthersInhibitorinhibitorinhibit

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Z-321

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Complete Stock Solution Preparation Table

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