SMI-4a (Synonyms: TCS-PIM-1-4a)

Name: SMI-4a
Brand: MedChemExpress
SKU: HY-16576A
Rating: 4.5 (1 reviews)

Cat. No.: HY-16576A Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SMI-4a (TCS-PIM-1-4a) is a poten, selective, cell-permeable and ATP-competitive Pim-1 inhibitor with an IC₅₀ of 24 μM and a K_i of 0.6 μM. SMI-4a also inhibits Pim-2 (IC₅₀ of 100 μM), and does not significantly inhibit the other serine/threonine- or tyrosine-kinases. SMI-4a has anticancer activity.

For research use only. We do not sell to patients.

SMI-4a Chemical Structure

CAS No. : 438190-29-5

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5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of SMI-4a:

(E/Z)-SMI-4a In-stock

1 Publications Citing Use of MCE SMI-4a

•J Virol. 2024 Jun 13:e0056124. [Abstract]

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PIM1 PIM2 PIM3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

PIM1

24 μM (IC₅₀)

PIM1

0.6 μM (Ki)

PIM2

100 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	17.8 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	31.4 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	37.4 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	40.1 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	36.8 μM Compound: 4	Inhibition of growth of HCT116 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	45.5 μM Compound: 4	Inhibition of growth of HCT116 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	48.8 μM Compound: 4	Inhibition of growth of HCT116 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	91.7 μM Compound: 4	Inhibition of growth of HCT116 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-8	IC₅₀	100 μM Compound: 4	Inhibition of growth of HCT-8 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT-8 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-8	IC₅₀	82.2 μM Compound: 4	Inhibition of growth of HCT-8 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT-8 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HL-60	GI₅₀	4.9 μM Compound: SMI-4a	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
HT-29	IC₅₀	31.4 μM Compound: 4	Inhibition of growth of HT-29 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	38.8 μM Compound: 4	Inhibition of growth of HT-29 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	59.7 μM Compound: 4	Inhibition of growth of HT-29 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	71.7 μM Compound: 4	Inhibition of growth of HT-29 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
MOLM-13	GI₅₀	2.16 μM Compound: SMI-4a	Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
Mononuclear cell line	GI₅₀	8.35 μM Compound: SMI-4a	Antiproliferative activity against AML patient derived mononuclear cells of assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against AML patient derived mononuclear cells of assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
PC-3	IC₅₀	17 μM Compound: 4a	Antiproliferative activity against human PC3 cells after 48 hrs by MTS assay Antiproliferative activity against human PC3 cells after 48 hrs by MTS assay	[PMID: 19072652]

In Vitro

SMI-4a (0.5 μM; 1 hour; HEK-293T cells) treatment attenuates the autophosphorylation of tagged Pim-1 in intact cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK-293T cells
Concentration:	0.5 µM
Incubation Time:	1 hour
Result:	Caused a dose-dependent reduction in Pim-1-induced 4E-BP1 phosphorylation, with an IC₅₀ of approximately 125 nM.

Molecular Weight

273.23

Formula

C₁₁H₆F₃NO₂S

CAS No.

438190-29-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC/1=O)SC1=C/C2=CC=CC(C(F)(F)F)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (365.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6599 mL	18.2996 mL	36.5992 mL
5 mM	0.7320 mL	3.6599 mL	7.3198 mL
10 mM	0.3660 mL	1.8300 mL	3.6599 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 2 mg/mL (7.32 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 20% HP-β-CD in Saline
Solubility: 5 mg/mL (18.30 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (251 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Xia Z, et al. Synthesis and evaluation of novel inhibitors of Pim-1 and Pim-2 protein kinases. J Med Chem. 2009 Jan 8;52(1):74-86. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6599 mL	18.2996 mL	36.5992 mL	91.4980 mL
	5 mM	0.7320 mL	3.6599 mL	7.3198 mL	18.2996 mL
	10 mM	0.3660 mL	1.8300 mL	3.6599 mL	9.1498 mL
	15 mM	0.2440 mL	1.2200 mL	2.4399 mL	6.0999 mL
	20 mM	0.1830 mL	0.9150 mL	1.8300 mL	4.5749 mL
	25 mM	0.1464 mL	0.7320 mL	1.4640 mL	3.6599 mL
	30 mM	0.1220 mL	0.6100 mL	1.2200 mL	3.0499 mL
	40 mM	0.0915 mL	0.4575 mL	0.9150 mL	2.2875 mL
	50 mM	0.0732 mL	0.3660 mL	0.7320 mL	1.8300 mL
	60 mM	0.0610 mL	0.3050 mL	0.6100 mL	1.5250 mL
	80 mM	0.0457 mL	0.2287 mL	0.4575 mL	1.1437 mL
	100 mM	0.0366 mL	0.1830 mL	0.3660 mL	0.9150 mL

SMI-4a Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMI-4a438190-29-5TCS-PIM-1-4aPimPim kinasesPim-1Pim-24E-BP1autophosphorylationcell-permeableanticancerInhibitorinhibitorinhibit

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SMI-4a (Synonyms: TCS-PIM-1-4a)

Customer Review

Other Forms of SMI-4a:

SMI-4a Related Antibodies

1 Publications Citing Use of MCE SMI-4a

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Pim Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

SMI-4a Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SMI-4a Related Classifications

Keywords:

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