(+)-SJ733 (Synonyms: SJ000557733)

Name: (+)-SJ733
Brand: MedChemExpress
SKU: HY-19556
Rating: 5.0 (1 reviews)

Cat. No.: HY-19556 Purity: 99.45% ee.: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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(+)-SJ733 is an anti-malaria agent which can also inhibit Na⁺-ATPase PfATP4.

For research use only. We do not sell to patients.

(+)-SJ733 Chemical Structure

CAS No. : 1424799-20-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 587	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 587	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 570	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 912	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2736	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4370	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

(+)-SJ733 is an anti-malaria agent which can also inhibit Na⁺-ATPase PfATP4.

IC₅₀ & Target

Plasmodium

In Vitro

(+)-SJ733 binds to a single receptor site in P. falciparum-infected erythrocytes with equivalent affinity to its growth-inhibitory potency (k_d=50 nM). (+)-SJ733 has not exhibited either significant safety liabilities at any dose in extensive profiling in vitro or significant safety or tolerability liabilities in either single- or repeat-dose studies at any dose tested in any preclinical species (no observed adverse effect level and maximum tolerated dose >240 mg/kg from 7-d repeat dosing study in rat). Therefore, (+)-SJ733 is expected to have a safety margin of at least 43-fold^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment of P. falciparum-infected NOD-scid IL2Rγ^null mice with (+)-SJ733 causes rapid clearance of parasites, which are 80% depleted within the first 24 h and undetectable by 48 h. (+)-SJ733 is highly potent and efficacious against P. falciparum 3D7^0087/N9 in vivo when administered as four sequential daily oral doses in the NOD-scid IL2Rγ^null mouse model, with a 90% effective dose, (ED₉₀ 1.9 mg/kg) and exposure [area under the curve at ED₉₀ (AUC_ED90), 1.5 μM⋅h] superior to artesunate (11.1 mg/kg; AUC_ED90 not determined), chloroquine (4.3 mg/kg; AUC_ED90 3.1 μM⋅h), and pyrimethamine (0.9 mg/kg; AUC_ED90 5. μM⋅h) in the same model. When treated with the ED₉₀ dose, (+)-SJ733 concentrations in blood remain above the average in vitro EC₉₀ for 6 to 10 h after each dose^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

468.40

Formula

C₂₄H₁₆F₄N₄O₂

CAS No.

1424799-20-1

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](C1=C2C=CC=C1)[C@@H](C3=CC=CN=C3)N(CC(F)(F)F)C2=O)NC4=CC=C(F)C(C#N)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (106.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1349 mL	10.6746 mL	21.3493 mL
5 mM	0.4270 mL	2.1349 mL	4.2699 mL
10 mM	0.2135 mL	1.0675 mL	2.1349 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45% ee.: 99.88%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (253 KB) HNMR (206 KB) RP-HPLC (265 KB) LCMS (400 KB)

Handling Instructions (2659 KB)

References

[1]. Jimenez-Diaz MB, et al. (+)-SJ733, a clinical candidate for malaria that acts through ATP4 to induce rapid host-mediated clearance of Plasmodium. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):E5455-62. [Content Brief]

Cell Assay ^[1]	10 mL of asynchronous culture suspensions (2% hematocrit), at different parasite densities (104, 105, 106, 107, and 108 parasites), are added to each well of a 6-well plate. (+)-SJ733 is added to each well to make a final compound concentration of 1.8 μM, corresponding to 30×EC₅₀ of the compound. Three wells are used for each parasite density. Plates are incubated at 37° C under an atmosphere of 90% N₂, 5% O₂, 5% CO₂ for 90 days under constant drug pressure. The media of each well is replaced 3 times a week with freshly made media containing a compound concentration of 30×EC₅₀. In addition, each well is split (1:2) once a week. Parasite outgrowth is monitored 3 times a week by transferring quadruplicate 40 μL aliquots from each well into a 384-well assay plate and determining parasitemia by a previously described method^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	The pharmacokinetics of (+)-SJ733 are studied in overnight-fasted male Sprague Dawley rats weighing 267 to 291 g predose. Rats have access to water ad libitum throughout the pre- and post-dose sampling period, and access to food is reinstated 4 h post-dose. (+)-SJ733 is administered intravenously as a 10 min constant rate infusion (1.0 mL per rat, n=3 rats) and orally by gavage (10 mL/kg, n=3 rats). The IV formulation consists of pH 7.4 isotonic phosphate buffered saline containing 1% (w/v) hydroxypropyl-β-cyclodextrin, 10% (v/v) ethanol, 10% (v/v) propylene glycol and 40% (v/v) PEG400 whereas the oral formulation is an aqueous suspension in 0.5% (w/v) hydroxypropyl methylcellulose, 0.5% (v/v) benzyl alcohol and 0.4% (v/v) Tween80. Aliquots of the formulations are retained for analysis of the actual dose administered. Samples of arterial blood and total urine are collected at various time points up to 24 h post-dose^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Jimenez-Diaz MB, et al. (+)-SJ733, a clinical candidate for malaria that acts through ATP4 to induce rapid host-mediated clearance of Plasmodium. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):E5455-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1349 mL	10.6746 mL	21.3493 mL	53.3732 mL
	5 mM	0.4270 mL	2.1349 mL	4.2699 mL	10.6746 mL
	10 mM	0.2135 mL	1.0675 mL	2.1349 mL	5.3373 mL
	15 mM	0.1423 mL	0.7116 mL	1.4233 mL	3.5582 mL
	20 mM	0.1067 mL	0.5337 mL	1.0675 mL	2.6687 mL
	25 mM	0.0854 mL	0.4270 mL	0.8540 mL	2.1349 mL
	30 mM	0.0712 mL	0.3558 mL	0.7116 mL	1.7791 mL
	40 mM	0.0534 mL	0.2669 mL	0.5337 mL	1.3343 mL
	50 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0675 mL
	60 mM	0.0356 mL	0.1779 mL	0.3558 mL	0.8896 mL
	80 mM	0.0267 mL	0.1334 mL	0.2669 mL	0.6672 mL
	100 mM	0.0213 mL	0.1067 mL	0.2135 mL	0.5337 mL

(+)-SJ733 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(+)-SJ7331424799-20-1SJ000557733SJ 000557733SJ-000557733Na+/K+ ATPaseParasiteSodium potassium pumpInhibitorinhibitorinhibit

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(+)-SJ733 (Synonyms: SJ000557733)

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Biological Activity

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References

Customer Review

(+)-SJ733 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

(+)-SJ733 Related Classifications

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