AC-099 hydrochloride

Cat. No.: HY-154829A Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

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AC-099 hydrochloride (compound 3) is a selective NPFF2R full agonist (EC₅₀=1189 nM) and NPFF1R partial agonist (EC₅₀=2370 nM). AC-099 hydrochloride attenuates spinal nerve ligation-induced hypersensitivity in rats.

For research use only. We do not sell to patients.

AC-099 hydrochloride Chemical Structure

CAS No. : 849335-07-5

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 255	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Other Forms of AC-099 hydrochloride:

AC-099 Get quote

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NPFFR1 NPFFR2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

NPFF2R/1R^[1]^[2].

In Vivo

AC-099 hydrochloride (compound 3; 30 mg/kg; i.p.; single) completely attenuates spinal nerve ligation (SNL)-induced hypersensitivity in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (175-300g; spinal nerve ligation (SNL) model)^[2].
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection; single.
Result:	Completely attenuated SNL-induced hypersensitivity.

Molecular Weight

301.10

Formula

C₉H₉Cl₂F₃N₄

CAS No.

849335-07-5

Appearance

Solid

Color

White to off-white

SMILES

FC(C1=CC(/C=N/NC(N)=N)=CC=C1Cl)(F)F.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (332.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3212 mL	16.6058 mL	33.2116 mL
5 mM	0.6642 mL	3.3212 mL	6.6423 mL
10 mM	0.3321 mL	1.6606 mL	3.3212 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (246 KB) HNMR (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Findeisen M, et al. Selective mode of action of guanidine-containing non-peptides at human NPFF receptors. J Med Chem. 2012 Jul 12;55(13):6124-36. [Content Brief]

[2]. Gaubert G, et al. Discovery of selective nonpeptidergic neuropeptide FF2 receptor agonists. J Med Chem. 2009 Nov 12;52(21):6511-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3212 mL	16.6058 mL	33.2116 mL	83.0289 mL
	5 mM	0.6642 mL	3.3212 mL	6.6423 mL	16.6058 mL
	10 mM	0.3321 mL	1.6606 mL	3.3212 mL	8.3029 mL
	15 mM	0.2214 mL	1.1071 mL	2.2141 mL	5.5353 mL
	20 mM	0.1661 mL	0.8303 mL	1.6606 mL	4.1514 mL
	25 mM	0.1328 mL	0.6642 mL	1.3285 mL	3.3212 mL
	30 mM	0.1107 mL	0.5535 mL	1.1071 mL	2.7676 mL
	40 mM	0.0830 mL	0.4151 mL	0.8303 mL	2.0757 mL
	50 mM	0.0664 mL	0.3321 mL	0.6642 mL	1.6606 mL
	60 mM	0.0554 mL	0.2768 mL	0.5535 mL	1.3838 mL
	80 mM	0.0415 mL	0.2076 mL	0.4151 mL	1.0379 mL
	100 mM	0.0332 mL	0.1661 mL	0.3321 mL	0.8303 mL