Allylthiourea (Synonyms: Thiosinamine; N-Allylthiourea)

Cat. No.: HY-B0543 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Allylthiourea can selectively inhibit the oxidation of ammonia. Allylthiourea is commonly used to inhibit nitrification by targeting ammonia monooxygenase and chelating copper in the active site to suppress its activity. Allylthiourea also exhibits anticancer activity, showing cytotoxicity against the MCF-7 cell line with an IC₅₀ of 5.22 mM. Allylthiourea can be utilized in research related to micropollutant biodegradability and cancer studies.

For research use only. We do not sell to patients.

Allylthiourea Chemical Structure

CAS No. : 109-57-9

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25 g	USD 35	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Allylthiourea:

Allylthiourea (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Allylthiourea (10 μM, 10 min) selectively inhibits ammonia oxidizers in vitro^[1].
Allylthiourea (15 μM, 70 h) decreases intracellular copper in Methylosinus trichosporium OB3b, allowing sMMO production at Cu/biomass ratios normally not permitting sMMO synthesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

116.18

Formula

C₄H₈N₂S

CAS No.

109-57-9

Appearance

Solid

Color

White to off-white

SMILES

S=C(N)NCC=C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (860.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.6073 mL	43.0367 mL	86.0733 mL
5 mM	1.7215 mL	8.6073 mL	17.2147 mL
10 mM	0.8607 mL	4.3037 mL	8.6073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (479 KB)

COA (243 KB) HNMR (232 KB)

Handling Instructions (2659 KB)

References

[1]. Ginestet, P., et al., Estimation of nitrifying bacterial activities by measuring oxygen uptake in the presence of the metabolic inhibitors allylthiourea and azide. Appl Environ Microbiol, 1998. 64(6): p. 2266-8. [Content Brief]

[2]. Yu, Y., J.A. Ramsay, and B.A. Ramsay, Use of allylthiourea to produce soluble methane monooxygenase in the presence of copper. Appl Microbiol Biotechnol, 2009. 82(2): p. 333-9. [Content Brief]

[3]. Tatari K, et al. Challenges in using allylthiourea and chlorate as specific nitrification inhibitors[J]. Chemosphere, 2017, 182: 301-305. [Content Brief]

[4]. Widiandani T, et al. New N-allylthiourea derivatives: synthesis, molecular docking and in vitro cytotoxicity studies[J]. Tropical Journal of Pharmaceutical Research, 2018, 17(8): 1607-1613.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	8.6073 mL	43.0367 mL	86.0733 mL	215.1833 mL
	5 mM	1.7215 mL	8.6073 mL	17.2147 mL	43.0367 mL
	10 mM	0.8607 mL	4.3037 mL	8.6073 mL	21.5183 mL
	15 mM	0.5738 mL	2.8691 mL	5.7382 mL	14.3456 mL
	20 mM	0.4304 mL	2.1518 mL	4.3037 mL	10.7592 mL
	25 mM	0.3443 mL	1.7215 mL	3.4429 mL	8.6073 mL
	30 mM	0.2869 mL	1.4346 mL	2.8691 mL	7.1728 mL
	40 mM	0.2152 mL	1.0759 mL	2.1518 mL	5.3796 mL
	50 mM	0.1721 mL	0.8607 mL	1.7215 mL	4.3037 mL
	60 mM	0.1435 mL	0.7173 mL	1.4346 mL	3.5864 mL
	80 mM	0.1076 mL	0.5380 mL	1.0759 mL	2.6898 mL
	100 mM	0.0861 mL	0.4304 mL	0.8607 mL	2.1518 mL

Allylthiourea Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allylthiourea109-57-9Thiosinamine N-AllylthioureaReactive Oxygen Speciesoxidation of ammonianitrificationMCF-7 cell linemicropollutant biodegradabilityInhibitorinhibitorinhibit

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