alpha-1,3-Fucosyltransferase (α1,3FucT) 

alpha-1,3-Fucosyltransferase (α1,3FucT) catalyzes the transfer of L-fucose moiety from guanosine diphosphate-beta-L-fucose (GDP-Fuc) to acceptor sugars. alpha-1,3-Fucosyltransferase (α1,3FucT) is often used in biochemical studies, and it can be used to form fucoglycoconjugates^[1].

Product Information
This product is recombinantly expressed in E. coli, with a C-terminal His Tag, a molecular weight of 37 kDa, and a storage buffer component of 20 mM Tris-HCl, pH 8.0, 10% glycerol. It can be used to connect fucose to the sugar chain of Galβ1-3GlcOR in an α1,3 connection manner, and is a key enzyme that catalyzes the synthesis of 3'-fucosyllactose.

Instructions
1. Add substrate according to the reaction system, 10 mM MnCl₂, and appropriate buffer salts (such as Tris-HCl) can be added to adjust the pH to 7.5, and add appropriate amount of enzyme (according to reaction requirements);
2. After mixing, incubate the sample at 37℃ for 2-4h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37277-69-3

Liquid

Colorless to light yellow

[alpha-1,3-Fucosyltransferase]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Murray BW, et al. Mechanism and specificity of human alpha-1,3-fucosyltransferase V. Biochemistry. 1996 Aug 27;35(34):11183-95.  [Content Brief]