1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. alpha-1,3-Fucosyltransferase (α1,3FucT)

alpha-1,3-Fucosyltransferase (α1,3FucT) catalyzes the transfer of L-fucose moiety from guanosine diphosphate-beta-L-fucose (GDP-Fuc) to acceptor sugars. alpha-1,3-Fucosyltransferase (α1,3FucT) is often used in biochemical studies, and it can be used to form fucoglycoconjugates.

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alpha-1,3-Fucosyltransferase (α1,3FucT) Chemical Structure

alpha-1,3-Fucosyltransferase (α1,3FucT) Chemical Structure

CAS No. : 37277-69-3

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Description

alpha-1,3-Fucosyltransferase (α1,3FucT) catalyzes the transfer of L-fucose moiety from guanosine diphosphate-beta-L-fucose (GDP-Fuc) to acceptor sugars. alpha-1,3-Fucosyltransferase (α1,3FucT) is often used in biochemical studies, and it can be used to form fucoglycoconjugates[1].

In Vitro

Product Information
This product is recombinantly expressed in E. coli, with a C-terminal His Tag, a molecular weight of 37 kDa, and a storage buffer component of 20 mM Tris-HCl, pH 8.0, 10% glycerol. It can be used to connect fucose to the sugar chain of Galβ1-3GlcOR in an α1,3 connection manner, and is a key enzyme that catalyzes the synthesis of 3'-fucosyllactose.

Instructions
1. Add substrate according to the reaction system, 10 mM MnCl2, and appropriate buffer salts (such as Tris-HCl) can be added to adjust the pH to 7.5, and add appropriate amount of enzyme (according to reaction requirements);
2. After mixing, incubate the sample at 37℃ for 2-4h.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[alpha-1,3-Fucosyltransferase]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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alpha-1,3-Fucosyltransferase (α1,3FucT) Related Classifications

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Product Name:
alpha-1,3-Fucosyltransferase (α1,3FucT)
Cat. No.:
HY-E70064
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