Anagyrine (Synonyms: (-)-Anagyrine; Monolupine; Rhombinine)

Cat. No.: HY-121027 Purity: 96.77%: FAQs
Data Sheet SDS COA Handling Instructions

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Anagyrine ((-)-Anagyrine) is a quinolizidine alkaloid that has been found in Lupinus albus. Anagyrine binds to muscarinic and nicotinic acetylcholine receptors with IC₅₀ values of 132 and 2096 µM respectively. Anagyrine is a potent and effective desensitizer of nAChR, and Anagyrine can directly, without metabolism, desensitize nAChR.

For research use only. We do not sell to patients.

Anagyrine Chemical Structure

CAS No. : 486-89-5

Size	Price	Stock	Quantity
1 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1150	Get quote	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Other Forms of Anagyrine:

Anagyrine hydrochloride Get quote

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•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

View All Isoforms

mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Anagyrine acts as a partial agonist in both cell lines with EC₅₀ values of 4.2 and 231 μM in SH-SY5Y and TE-671 cells, respectively. Anagyrine is a desensitizer of nAChR with DC₅₀ values of 6.9 and 139 μM in SH-SY5Y and TE-671 cells, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

244.33

Formula

C₁₅H₂₀N₂O

CAS No.

486-89-5

Appearance

Oil

Color

Colorless to light yellow

SMILES

O=C1N2C([C@]3([H])C[C@]([C@]4([H])N(CCCC4)C3)([H])C2)=CC=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (102.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (102.32 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0928 mL	20.4641 mL	40.9283 mL
5 mM	0.8186 mL	4.0928 mL	8.1857 mL
10 mM	0.4093 mL	2.0464 mL	4.0928 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Saline
Solubility: ≥ 2 mg/mL (8.19 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 97.05%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) HNMR (203 KB) RP-HPLC (73 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Schmeller T, et al. Binding of quinolizidine alkaloids to nicotinic and muscarinic acetylcholine receptors. J Nat Prod. 1994 Sep;57(9):1316-9. [Content Brief]

[2]. Matsuda, K., et al. Nematicidal activities of (–)-N-methylcytisine and (–)-anagyrine from Sophora flavescens against pine wood nematodes. Agr. BioI. Chem. 53(8), 2287-2288 (1989).

[3]. Green BT, et al. Anagyrine desensitization of peripheral nicotinic acetylcholine receptors. A potential biomarker of quinolizidine alkaloid teratogenesis in cattle. Res Vet Sci. 2017 Dec;115:195-200. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.0928 mL	20.4641 mL	40.9283 mL	102.3206 mL
	5 mM	0.8186 mL	4.0928 mL	8.1857 mL	20.4641 mL
	10 mM	0.4093 mL	2.0464 mL	4.0928 mL	10.2321 mL
	15 mM	0.2729 mL	1.3643 mL	2.7286 mL	6.8214 mL
	20 mM	0.2046 mL	1.0232 mL	2.0464 mL	5.1160 mL
	25 mM	0.1637 mL	0.8186 mL	1.6371 mL	4.0928 mL
	30 mM	0.1364 mL	0.6821 mL	1.3643 mL	3.4107 mL
	40 mM	0.1023 mL	0.5116 mL	1.0232 mL	2.5580 mL
	50 mM	0.0819 mL	0.4093 mL	0.8186 mL	2.0464 mL
	60 mM	0.0682 mL	0.3411 mL	0.6821 mL	1.7053 mL
	80 mM	0.0512 mL	0.2558 mL	0.5116 mL	1.2790 mL
	100 mM	0.0409 mL	0.2046 mL	0.4093 mL	1.0232 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Anagyrine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anagyrine486-89-5(-)-Anagyrine Monolupine RhombininemAChRnAChRMuscarinic acetylcholine receptorNicotinic acetylcholine receptorsSH-SY5Y cellsTE-671 cellsteratogenicquinolizidinemuscarinicdesensitizerInhibitorinhibitorinhibit

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