Anhydrotetracycline hydrochloride

Name: Anhydrotetracycline hydrochloride
Brand: MedChemExpress
SKU: HY-118660
Rating: 4.5 (1 reviews)

Cat. No.: HY-118660 Purity: 99.68%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Anhydrotetracycline hydrochloride, a tetracycline biosynthetic precursor, is a potent competitive broad-spectrum tetracycline destructase enzymes inhibitor. Anhydrotetracycline hydrochloride is an effector for tetracycline controlled gene expression systems in eukaryotic cells.

For research use only. We do not sell to patients.

Anhydrotetracycline hydrochloride Chemical Structure

CAS No. : 13803-65-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 97	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 97	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 238	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 333	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 466	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Anhydrotetracycline hydrochloride:

Anhydrotetracycline Get quote
Anhydrotetracycline hydrochloride (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Tetracycline

In Vitro

Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=210 μM),Tet(X) (IC₅₀=41 μM), Tet(X)_3 (IC₅₀=3 μM) degradations of tetracyclines. Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=210 μM),Tet(X) (IC₅₀=75 μM), Tet(X)_3 (IC₅₀=26 μM) degradations of chlortetracycline. Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=120 μM),Tet(X) (IC₅₀=41 μM), Tet(X)_3 (IC₅₀=7 μM) degradations of demeclocycline^[1].
Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=210 μM),Tet(X) (IC₅₀=41 μM), Tet(X)_3 (IC₅₀=3 μM) degradations of tetracyclines. Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=210 μM),Tet(X) (IC₅₀=75 μM), Tet(X)_3 (IC₅₀=26 μM) degradations of chlortetracycline. Anhydrotetracycline hydrochloride inhibits Tet(50) (IC₅₀=120 μM),Tet(X) (IC₅₀=41 μM), Tet(X)_3 (IC₅₀=7 μM) degradations of demeclocycline^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

462.88

Formula

C₂₂H₂₃ClN₂O₇

CAS No.

13803-65-1

Appearance

Solid

Color

Yellow to brown

SMILES

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])CC3=C(C([C@@]21O)=O)C(O)=C4C(O)=CC=CC4=C3C)N.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 170 mg/mL (367.27 mM; Need ultrasonic)

DMSO : 100 mg/mL (216.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1604 mL	10.8019 mL	21.6039 mL
5 mM	0.4321 mL	2.1604 mL	4.3208 mL
10 mM	0.2160 mL	1.0802 mL	2.1604 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (4.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.68%

Select Batch:

Data Sheet (277 KB) SDS (557 KB)

COA (253 KB) HNMR (511 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Jana L Markley, et al. Semisynthetic Analogues of Anhydrotetracycline as Inhibitors of Tetracycline Destructase Enzymes. ACS Infect Dis. 2019 Apr 12;5(4):618-633. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.1604 mL	10.8019 mL	21.6039 mL	54.0097 mL
	5 mM	0.4321 mL	2.1604 mL	4.3208 mL	10.8019 mL
	10 mM	0.2160 mL	1.0802 mL	2.1604 mL	5.4010 mL
	15 mM	0.1440 mL	0.7201 mL	1.4403 mL	3.6006 mL
	20 mM	0.1080 mL	0.5401 mL	1.0802 mL	2.7005 mL
	25 mM	0.0864 mL	0.4321 mL	0.8642 mL	2.1604 mL
	30 mM	0.0720 mL	0.3601 mL	0.7201 mL	1.8003 mL
	40 mM	0.0540 mL	0.2700 mL	0.5401 mL	1.3502 mL
	50 mM	0.0432 mL	0.2160 mL	0.4321 mL	1.0802 mL
	60 mM	0.0360 mL	0.1800 mL	0.3601 mL	0.9002 mL
	80 mM	0.0270 mL	0.1350 mL	0.2700 mL	0.6751 mL
	100 mM	0.0216 mL	0.1080 mL	0.2160 mL	0.5401 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.