Antineoplaston A10

Name: Antineoplaston A10
Brand: MedChemExpress
SKU: HY-128553
Rating: 4.5 (1 reviews)

Cat. No.: HY-128553 Purity: 98.54%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Antineoplaston A10 is an antineoplaston that inhibits the growth of human hepatoma cells by inducing apoptosis. Antineoplaston A10 can be used in the study of liver cancer and breast cancer.

For research use only. We do not sell to patients.

Antineoplaston A10 Chemical Structure

CAS No. : 91531-30-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 127	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 127	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 880	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Antineoplaston A10:

(Rac)-Antineoplaston A10 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Antineoplaston A10 is an antineoplaston that inhibits the growth of human hepatoma cells by inducing apoptosis. Antineoplaston A10 can be used in the study of liver cancer and breast cancer^[1]^[2].

IC₅₀ & Target

Ras^[1].

In Vitro

Antineoplaston A10 (10-400 μg/ml; 0-120 h) inhibits the growth of human hepatoma cell lines HepG2 and HLE in a dose- and time-dependent manner and induces apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	HepG2 and HLE cell lines
Concentration:	10, 50, 100, 200 and 400 μg/ml
Incubation Time:	0-120 h
Result:	The cell growth inhibition rate was less than 10% at a dose of 10 μg/ml after incubation for 120 h, while the cell growth inhibition rate was 34.9% at a dose of 200 μg/ml after incubation for 120 h.

Apoptosis Analysis^[1]

Cell Line:	HepG2 and HLE cell lines
Concentration:	10, 50, 100, 200 and 400 μg/ml
Incubation Time:	0-120 h
Result:	Induced apoptosis in HepG2 cells at a dose of 50 μg/ml for 48 h.

In Vivo

Antineoplaston A10 (0-600 mg/kg; p.o.; 6 days per week for 5 weeks) dose-dependently inhibits the growth of HepG2 and HLE cells in mice and dose-dependently increases the incidence of apoptotic cells in xenograft mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Balb/c athymic (nu+/nu+) female mice with hepatocellular carcinoma xenografts^[1].
Dosage:	0, 150, 300 and 600 mg/kg
Administration:	Oral gavage (p.o.) 6 days per week for 5 weeks
Result:	Reduced the expression of bcl-2 in the cytoplasm, and the bcl-2 positive cell rates decreased to 82.9, 69.4 and 56.3%, respectively. Increased the expression of p53 in the nucleus, and the p53 positive cell rates increased to 20.6%, 36.2 and 60.5%, respectively.

Clinical Trial

Molecular Weight

246.26

Formula

C₁₃H₁₄N₂O₃

CAS No.

91531-30-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@@H](CC1)C(NC1=O)=O)CC2=CC=CC=C2

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1015.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0607 mL	20.3037 mL	40.6075 mL
5 mM	0.8121 mL	4.0607 mL	8.1215 mL
10 mM	0.4061 mL	2.0304 mL	4.0607 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.54%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (247 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Qu XJ, et al. Induction of apoptosis in human hepatocellular carcinoma cells by synthetic antineoplaston A10. Anticancer Res. 2007 Jul-Aug;27(4B):2427-31. [Content Brief]

[2]. Tsuda, et al. "Inhibitory effect of antineoplaston A-10 on breast cancer transplanted to athymic mice and human hepatocellular carcinoma cell lines." The Kurume Medical Journal 37.2 (1990): 97-104. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0607 mL	20.3037 mL	40.6075 mL	101.5187 mL
	5 mM	0.8121 mL	4.0607 mL	8.1215 mL	20.3037 mL
	10 mM	0.4061 mL	2.0304 mL	4.0607 mL	10.1519 mL
	15 mM	0.2707 mL	1.3536 mL	2.7072 mL	6.7679 mL
	20 mM	0.2030 mL	1.0152 mL	2.0304 mL	5.0759 mL
	25 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	30 mM	0.1354 mL	0.6768 mL	1.3536 mL	3.3840 mL
	40 mM	0.1015 mL	0.5076 mL	1.0152 mL	2.5380 mL
	50 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0304 mL
	60 mM	0.0677 mL	0.3384 mL	0.6768 mL	1.6920 mL
	80 mM	0.0508 mL	0.2538 mL	0.5076 mL	1.2690 mL
	100 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL