2. Anti-infection Membrane Transporter/Ion Channel Apoptosis
  3. Bacterial Fungal Na+/K+ ATPase Apoptosis Antibiotic
  4. Bafilomycin C1

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC).

For research use only. We do not sell to patients.

Bafilomycin C1 Chemical Structure

Bafilomycin C1 Chemical Structure

CAS No. : 88979-61-7

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Based on 1 publication(s) in Google Scholar

Bafilomycin C1 Related Antibodies

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1 Publications Citing Use of MCE Bafilomycin C1

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Description

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi[2]. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC)[2].

IC50 & Target

Macrolide

 

Cellular Effect
Cell Line Type Value Description References
BGC-823 IC50
1.83 μM
Compound: 7
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
Caco-2 IC50
1.89 μM
Compound: 7
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
NCI-H460 IC50
3.43 μM
Compound: 7
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
SMMC-7721 IC50
0.54 μM
Compound: 7
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
In Vitro

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner[2].
Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells[2].
Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bafilomycin C1 Related Antibodies

Cell Viability Assay[2]

Cell Line: SMMC7721 cell and HepG2 cell
Concentration: 0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721
1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time: 6 days
Result: Retarded the cell growth.

Western Blot Analysis[2]

Cell Line: SMMC7721 cells
Concentration: 3.3 μM
Incubation Time: 24 hours
Result: Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis[2]

Cell Line: SMMC7721 and HepG2 cells
Concentration: 3.3 μM; 10 μM
Incubation Time: 24 hours
Result: Induced apoptosis in SMMC7721 and HepG2 cells.
In Vivo

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×106 cells/100 μL)[2]
Dosage: 0.2 mg/kg
Administration: Subcutaneous injection; 20 days
Result: Suppressed tumor growth of SMMC7721 tumor xenografts.
Molecular Weight

720.89

Formula

C39H60O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H]([C@@](C[C@H]([C@@H]1C)OC(/C=C/C(O)=O)=O)(O[C@@H]1C(C)C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C/[C@H]2C)=O)([H])[C@H](/C=C/C=C(C[C@@H]([C@@H]2O)C)\C)OC)C
Structure Classification
Initial Source

Streptomyces sp
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (6.94 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3872 mL 6.9359 mL 13.8717 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.

  • Protocol 1

    Bafilomycin C1 (0.2 mg/kg body weight, dissolved in 60% 1,2-propanediol)[2]

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

SDS (420 KB)

COA (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3872 mL 6.9359 mL 13.8717 mL 34.6794 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL 6.9359 mL
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Bafilomycin C1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bafilomycin C188979-61-7Bafilomycin C 1Bafilomycin C-1BacterialFungalNa+/K+ ATPaseApoptosisAntibioticSodium potassium pumpprokaryoticeukaryoticSMMC7721HepatocellularcarcinomaHCChepaticchemotherapyInhibitorinhibitorinhibit

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