Betulinaldehyde (Synonyms: Betulinic aldehyde; Betunal)

Name: Betulinaldehyde
Brand: MedChemExpress
SKU: HY-N0084
Rating: 5.0 (7 reviews)

Cat. No.: HY-N0084 Purity: 98.72%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Betulinaldehyde (Betunal) Has anti-cancer and anti-staphylococcus aureus activity. Betulinaldehyde Suppressible Akt, MAPK sum STAT3 Signal path, increase self-transfer, Suppression A549 Cellular vitality, increase and transfer. Betulinaldehyde suppresses PLCγ1/Ca²⁺/MMP9 signal pathway, has a key effect on vascular plasticity, and is available for cardiovascular disease (CVD) research.

For research use only. We do not sell to patients.

Betulinaldehyde Chemical Structure

CAS No. : 13159-28-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 122	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 122	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 111	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 178	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 721	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1141	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 7 publication(s) in Google Scholar

4 Publications Citing Use of MCE Betulinaldehyde

Cell Viability Assay

Proliferation Assay

: Betulinaldehyde purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jan 13;13(1):743. [Abstract]; The positive colony number of A549 cells is reduced by Betulinaldehyde concentrations of > 20 μM, while the colony formation ability of the A549 cells is almost completely inhibited when the concentration reaches 80 μM.

: Betulinaldehyde purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jan 13;13(1):743. [Abstract]; Betulinaldehyde (20 μM) for 60 min, the phosphorylated Akt level is significantly inhibited, while the activity of the Akt signaling pathway is almost completely inhibited after 180 min.

: Betulinaldehyde purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jan 13;13(1):743. [Abstract]; Betulinaldehyde (20, 40, 80 μM; 24 h) significantly inhibits viability of A549 cells.

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
B16	ED₅₀	3.3 μM Compound: 9	Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days	[PMID: 12027734]
B16	IC₅₀	6.4 μM Compound: 9	Growth inhibition of mouse B16 2F2 cells after 3 days Growth inhibition of mouse B16 2F2 cells after 3 days	[PMID: 12027734]
Epithelial cell	IC₅₀	19.4 μM Compound: betulinaldehyde	Antiproliferative activity against mouse +SA mammary epithelial cells after 4 days by MTT assay Antiproliferative activity against mouse +SA mammary epithelial cells after 4 days by MTT assay	[PMID: 18826277]

In Vitro

Betulinaldehyde (20, 40, 80 μM; 24 h) inhibits the colony formation ability of A549 cells in a concentration-dependent manner^[3].
Betulinaldehyde (20 μM; 24 h) inhibits the phosphorylation of Akt, MAPK and STAT3 in A549 cells^[3].
Betulinaldehyde (80 μM, 160 μM; 24 h) leads vascular remodeling in rats with carotid balloon injurys, and inhibits intracellular Ca2+ levels^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Betulinaldehyde (50-200 mg/kg; ip; once daily for 1 week) inhibits the growth of A549 tumor xenografs in nude mice in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

440.70

Formula

C₃₀H₄₈O₂

CAS No.

13159-28-9

Appearance

Solid

Color

White to off-white

SMILES

C=C(C)[C@@H]1CC[C@]2(C=O)CC[C@@]3(C)[C@]4(C)CC[C@@]5([H])C(C)(C)[C@@H](O)CC[C@]5(C)[C@@]4([H])CC[C@]3([H])[C@]21[H]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (45.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2691 mL	11.3456 mL	22.6912 mL
5 mM	0.4538 mL	2.2691 mL	4.5382 mL
10 mM	0.2269 mL	1.1346 mL	2.2691 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.72%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (253 KB) RP-HPLC (218 KB)

Handling Instructions (2659 KB)

References

[1]. Chung PY, et al. Identification, by gene expression profiling analysis, of novel gene targets in Staphylococcus aureus treated with betulinaldehyde. Res Microbiol. 2013 May;164(4):319-26. [Content Brief]

[2]. Chung PY, et al. Transcriptional profiles of the response of methicillin-resistant Staphylococcus aureus to pentacyclic triterpenoids. PLoS One. 2013;8(2):e56687. [Content Brief]

[3]. Huang PH, et al. Betulinaldehyde exhibits effective anti-tumor effects in A549 cells by regulating intracellular autophagy. Sci Rep. 2023 Jan 13;13(1):743. [Content Brief]

[4]. Fu Y, et al. Betulinaldehyde inhibits vascular remodeling by regulating the microenvironment through the PLCγ1/Ca2+/MMP9 pathway. Phytomedicine. 2023 Jul 25;116:154891. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2691 mL	11.3456 mL	22.6912 mL	56.7279 mL
	5 mM	0.4538 mL	2.2691 mL	4.5382 mL	11.3456 mL
	10 mM	0.2269 mL	1.1346 mL	2.2691 mL	5.6728 mL
	15 mM	0.1513 mL	0.7564 mL	1.5127 mL	3.7819 mL
	20 mM	0.1135 mL	0.5673 mL	1.1346 mL	2.8364 mL
	25 mM	0.0908 mL	0.4538 mL	0.9076 mL	2.2691 mL
	30 mM	0.0756 mL	0.3782 mL	0.7564 mL	1.8909 mL
	40 mM	0.0567 mL	0.2836 mL	0.5673 mL	1.4182 mL

Betulinaldehyde Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Betulinaldehyde13159-28-9Betulinic aldehyde BetunalBacterialInhibitorinhibitorinhibit

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Betulinaldehyde (Synonyms: Betulinic aldehyde; Betunal)

Customer Review

Betulinaldehyde Related Antibodies

4 Publications Citing Use of MCE Betulinaldehyde

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Betulinaldehyde Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Betulinaldehyde Related Classifications

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