2. GPCR/G Protein Neuronal Signaling
  3. Neuropeptide Y Receptor
  4. BIBO3304

BIBO3304 is a potent, orally active, and selective neuropeptide Y (NPY) Y1 receptor antagonist, with subnanomolar affinity for both the human and the rat Y1 receptor (IC50=0.38 and 0.72 nM, respectively).

For research use only. We do not sell to patients.

BIBO3304 Chemical Structure

BIBO3304 Chemical Structure

CAS No. : 191868-14-1

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1 mg USD 75 In-stock
5 mg USD 180 In-stock
10 mg USD 328 In-stock
25 mg USD 740 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of BIBO3304:

BIBO3304 Related Antibodies

Top Publications Citing Use of Products

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NPY Y1 receptor NPY Y2 receptor NPY Y4 receptor NPY Y5 receptor

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BIBO3304 is a potent, orally active, and selective neuropeptide Y (NPY) Y1 receptor antagonist, with subnanomolar affinity for both the human and the rat Y1 receptor (IC50=0.38 and 0.72 nM, respectively)[1].

IC50 & Target

NPY Y1 receptor

 

In Vivo

BIBO3304 (30 μg; bilateral paraventricular nucleus injection) attenuates the hyperphagia following fasting[1].
BIBO3304 (15-60 μg) dose-dependently inhibits the feeding reponse mediated by 1 μg NPY[1].
BIBO3304 (0.5 μM; p.o.) significantly increases serum insulin levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male Chbb:Thom rats weighing between 300 and 340 g[1]
Dosage: 30 μg
Administration: bilateral paraventricular nucleus injection
Result: Attenuated the hyperphagia following fasting, especially during the first 2 h of refeeding.
Animal Model: 7-week-old C57BL/6JAusb mice[2]
Dosage: 0.5 μM
Administration: p.o.
Result: Significantly increased serum insulin levels.
Molecular Weight

643.66

Formula

C31H36F3N7O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@@H](C(NCC1=CC=C(CNC(N)=O)C=C1)=O)CCCNC(N)=N)C(C2=CC=CC=C2)C3=CC=CC=C3.O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (155.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5536 mL 7.7681 mL 15.5362 mL
5 mM 0.3107 mL 1.5536 mL 3.1072 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.88 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.88 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.95%

COA (252 KB) LCMS (91 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5536 mL 7.7681 mL 15.5362 mL 38.8404 mL
5 mM 0.3107 mL 1.5536 mL 3.1072 mL 7.7681 mL
10 mM 0.1554 mL 0.7768 mL 1.5536 mL 3.8840 mL
15 mM 0.1036 mL 0.5179 mL 1.0357 mL 2.5894 mL
20 mM 0.0777 mL 0.3884 mL 0.7768 mL 1.9420 mL
25 mM 0.0621 mL 0.3107 mL 0.6214 mL 1.5536 mL
30 mM 0.0518 mL 0.2589 mL 0.5179 mL 1.2947 mL
40 mM 0.0388 mL 0.1942 mL 0.3884 mL 0.9710 mL
50 mM 0.0311 mL 0.1554 mL 0.3107 mL 0.7768 mL
60 mM 0.0259 mL 0.1295 mL 0.2589 mL 0.6473 mL
80 mM 0.0194 mL 0.0971 mL 0.1942 mL 0.4855 mL
100 mM 0.0155 mL 0.0777 mL 0.1554 mL 0.3884 mL
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BIBO3304 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BIBO3304191868-14-1BIBO 3304BIBO-3304Neuropeptide Y ReceptorNPY receptororalsubtypeseruminsulinhyperphagiarefeedingInhibitorinhibitorinhibit

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