Biflorin

Name: Biflorin
Brand: MedChemExpress
SKU: HY-119956
Rating: 4.5 (1 reviews)

Cat. No.: HY-119956 Purity: 99.54%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Biflorin has antimicrobial, antitumor, antiinflammatory and antimutagenic activities. Biflorin is found in the roots of Capraria biflora L.

For research use only. We do not sell to patients.

Biflorin Chemical Structure

CAS No. : 89701-85-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote	Estimated Time of Arrival: December 31
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50 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Biflorin has antimicrobial, antitumor, antiinflammatory and antimutagenic activities. Biflorin is found in the roots of Capraria biflora L^[1]^[2]^[3].

IC₅₀ & Target^[2]

STAT1

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	140 μM Compound: 1	Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 26977531]
RAW264.7	IC₅₀	37.1 μM Compound: 1	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS challenge for 24 hrs by ELISA method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS challenge for 24 hrs by ELISA method	[PMID: 26977531]
RAW264.7	IC₅₀	51.7 μM Compound: 1	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 1 hr followed by LPS challenge for 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 1 hr followed by LPS challenge for 24 hrs by Griess assay	[PMID: 26977531]

In Vitro

Biflorin (72 h) inhibits tumor cell growth with IC₅₀s ranging from 0.58 μg/mL (NCIH23 cell) to 14.61 μg/mL (MDA-MB-231 cell)^[1].
Biflorin (3 and 6 μg/mL, 24 h) induces apoptosis, internucleosomal DNA fragmentation, mitochondrial depolarization in B16 melanoma cells^[1].
Biflorin inhibits LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages via STAT1 Inactivation, with IC₅₀s of 51.7 and 37.1 μM, respectively^[2].
Biflorin (5 and 10 μg/mL, 3 h) inhibits H₂O₂-induced oxidative damage by reducing lipid peroxidation and DNA damage in V79 mammalian cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Biflorin (25mg/day, i.p., for 10 days) inhibits tumor growth and increases the mean survival rate in B16 melanoma-bearing mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

354.31

Formula

C₁₆H₁₈O₉

CAS No.

89701-85-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)OC2=CC(O)=C([C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C(O)=C12

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (282.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8224 mL	14.1119 mL	28.2239 mL
5 mM	0.5645 mL	2.8224 mL	5.6448 mL
10 mM	0.2822 mL	1.4112 mL	2.8224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (251 KB) HNMR (251 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Vasconcellos MC, et al. The in-vitro and in-vivo inhibitory activity of biflorin in melanoma. Melanoma Res. 2011 Apr;21(2):106-14. [Content Brief]

[2]. Lee HH, et al. Biflorin, Isolated from the Flower Buds of Syzygium aromaticum L., Suppresses LPS-Induced Inflammatory Mediators via STAT1 Inactivation in Macrophages and Protects Mice from Endotoxin Shock. J Nat Prod. 2016 Apr 22;79(4):711-20. [Content Brief]

[3]. Vasconcellos MC,et al. Evaluation of the cytotoxic and antimutagenic effects of biflorin, an antitumor 1,4 o-naphthoquinone isolated from Capraria biflora L. Arch Toxicol. 2010 Oct;84(10):799-810. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8224 mL	14.1119 mL	28.2239 mL	70.5597 mL
	5 mM	0.5645 mL	2.8224 mL	5.6448 mL	14.1119 mL
	10 mM	0.2822 mL	1.4112 mL	2.8224 mL	7.0560 mL
	15 mM	0.1882 mL	0.9408 mL	1.8816 mL	4.7040 mL
	20 mM	0.1411 mL	0.7056 mL	1.4112 mL	3.5280 mL
	25 mM	0.1129 mL	0.5645 mL	1.1290 mL	2.8224 mL
	30 mM	0.0941 mL	0.4704 mL	0.9408 mL	2.3520 mL
	40 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7640 mL
	50 mM	0.0564 mL	0.2822 mL	0.5645 mL	1.4112 mL
	60 mM	0.0470 mL	0.2352 mL	0.4704 mL	1.1760 mL
	80 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8820 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7056 mL

Biflorin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biflorin89701-85-9ApoptosisSTATantimicrobialantitumorantiinflammatoryantimutagenicInhibitorinhibitorinhibit

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Biflorin

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Complete Stock Solution Preparation Table

Biflorin Related Classifications

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