Amprolium hydrochloride

Cat. No.: HY-B0937A Purity: 99.15%: FAQs
Data Sheet SDS COA Handling Instructions

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Amprolium hydrochloride is an anti-parasitic drug that's kind of taken orally, and it acts as an antagonist to thiamine in intestinal absorption.

For research use only. We do not sell to patients.

Amprolium hydrochloride Chemical Structure

CAS No. : 137-88-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
1 g	USD 110	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Other Forms of Amprolium hydrochloride:

Amprolium-d₇ bromide hydrobromide Get quote
Amprolium Get quote
Amprolium hydrochloride (Standard) Get quote

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Biological Activity
Purity & Documentation
References
Customer Review

Description

Amprolium hydrochloride is an anti-parasitic drug that's kind of taken orally, and it acts as an antagonist to thiamine in intestinal absorption^[1]^[2]^[3]^[4].

IC₅₀ & Target

Coccidia

In Vivo

Amprolium hydrochloride (1 g/L, oral, daily, for 7 days) can be used as a preventive measure in rabbits in combination with other anti-coccidial drugs^[1].
Amprolium hydrochloride (8 mg-2 g, taken orally or injected subcutaneously, daily, for 4-34 days) exerts an antagonistic effect with thiamine primarily at the level of the intestinal epithelial cells, impacting thiamine absorption. Once administered directly bypassing the gut, it does not significantly affect thiamine's entry into other tissue cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rabbits of white and California white breeds^[1]
Dosage:	1 g/L; daily; 7 days
Administration:	Oral
Result:	Had a good effect when used as preventive measures or combined with other antibody-resistant drugs.

Animal Model:	Rat with lack of thiamine^[2]
Dosage:	8 mg, daily, 4 days
Administration:	Oral
Result:	prevented the normal accumulation of thiamine in the tissue, and the antagonistic effect on the absorption of thumine was only existed at the intestinal level.

Animal Model:	Rat with lack of thiamine^[2]
Dosage:	2 g, daily, 34 days
Administration:	Subcutaneous injection (s.c.)
Result:	Did not prevent the deposition of thumine in the organs, and its level was close to the level reached by separate injection of thiamine.

Molecular Weight

315.24

Formula

C₁₄H₂₀Cl₂N₄

CAS No.

137-88-2

Appearance

Solid

Color

White to off-white

SMILES

CC1=CC=CC=[N+]1CC2=CN=C(CCC)N=C2N.[Cl-].Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 200 mg/mL (634.44 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1722 mL	15.8609 mL	31.7219 mL
5 mM	0.6344 mL	3.1722 mL	6.3444 mL
10 mM	0.3172 mL	1.5861 mL	3.1722 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (317.22 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.15%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) RP-HPLC (206 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Kennedy O Ogolla, et al. Efficacy of Sulphachloropyrazine, Amprolium Hydrochloride, Trimethoprim-Sulphamethoxazole, and Diclazuril against Experimental and Natural Rabbit Coccidiosis. J Vet Med. 2018 Oct 23:2018:5402469. [Content Brief]

[2]. G Rindi, et al. Action of Amprolium on the thiamine content of rat tissues. J Nutr. 1966 Jun;89(2):197-202. [Content Brief]

[3]. Iqbal A, et al. Antiparasitic efficacy of Artemisia absinthium, toltrazuril and amprolium against intestinal coccidiosis in goats. J Parasit Dis. 2013 Apr;37(1):88-93. [Content Brief]

[4]. Lindsay DS, et al. Effects of sulfadiazine and amprolium on Neospora caninum (Protozoa: Apicomplexa) infections in mice. J Parasitol. 1990 Apr;76(2):177-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.1722 mL	15.8609 mL	31.7219 mL	79.3047 mL
	5 mM	0.6344 mL	3.1722 mL	6.3444 mL	15.8609 mL
	10 mM	0.3172 mL	1.5861 mL	3.1722 mL	7.9305 mL
	15 mM	0.2115 mL	1.0574 mL	2.1148 mL	5.2870 mL
	20 mM	0.1586 mL	0.7930 mL	1.5861 mL	3.9652 mL
	25 mM	0.1269 mL	0.6344 mL	1.2689 mL	3.1722 mL
	30 mM	0.1057 mL	0.5287 mL	1.0574 mL	2.6435 mL
	40 mM	0.0793 mL	0.3965 mL	0.7930 mL	1.9826 mL
	50 mM	0.0634 mL	0.3172 mL	0.6344 mL	1.5861 mL
	60 mM	0.0529 mL	0.2643 mL	0.5287 mL	1.3217 mL
	80 mM	0.0397 mL	0.1983 mL	0.3965 mL	0.9913 mL
	100 mM	0.0317 mL	0.1586 mL	0.3172 mL	0.7930 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.