2. Cell Cycle/DNA Damage
  3. DNA Alkylator/Crosslinker
  4. Bizelesin

Bizelesin  (Synonyms: NSC 615291; U-77779)

Cat. No.: HY-111397 Purity: 95.07%
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Bizelesin (NSC 615291; U-77779) is an AT-specific DNA alkylating agent that can generate DNA interstrand crosslinks, effectively inhibit DNA replication, and has potential anticancer activity.

For research use only. We do not sell to patients.

Bizelesin Chemical Structure

Bizelesin Chemical Structure

CAS No. : 129655-21-6

Size Price Stock Quantity
1 mg USD 660 In-stock
5 mg USD 1670 In-stock
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50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Bizelesin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Bizelesin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Bizelesin (NSC 615291; U-77779) is an AT-specific DNA alkylating agent that can generate DNA interstrand crosslinks, effectively inhibit DNA replication, and has potential anticancer activity[1].

In Vitro

Bizelesin (0-5 μM, 4 h) can cause DNA-specific damage by targeting the AT-rich DNA domain in human cancer cell CEM cells, thereby causing damage to cancer cells, and has potential cancer therapeutic potential[1].
Bizelesin (0-500 nM) causes a 50% inhibition of DNA synthesis at a concentration of 10 nM, compared to a 50% inhibition of RNA synthesis at a concentration of 160 nM, at concentrations as high as 200 nM no inhibition of protein synthesis is observed in BSC-1 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bizelesin Related Antibodies
Clinical Trial
Molecular Weight

815.70

Formula

C43H36Cl2N8O5
CAS No.
Appearance

Solid

Color

Off-white to gray

SMILES

O=C(NC1=CC2=C(NC(C(N3C4=CC(O)=C5NC=C(C)C5=C4[C@H](CCl)C3)=O)=C2)C=C1)NC6=CC7=C(NC(C(N8C9=CC(O)=C%10NC=C(C)C%10=C9[C@H](CCl)C8)=O)=C7)C=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (122.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2259 mL 6.1297 mL 12.2594 mL
5 mM 0.2452 mL 1.2259 mL 2.4519 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2259 mL 6.1297 mL 12.2594 mL 30.6485 mL
5 mM 0.2452 mL 1.2259 mL 2.4519 mL 6.1297 mL
10 mM 0.1226 mL 0.6130 mL 1.2259 mL 3.0649 mL
15 mM 0.0817 mL 0.4086 mL 0.8173 mL 2.0432 mL
20 mM 0.0613 mL 0.3065 mL 0.6130 mL 1.5324 mL
25 mM 0.0490 mL 0.2452 mL 0.4904 mL 1.2259 mL
30 mM 0.0409 mL 0.2043 mL 0.4086 mL 1.0216 mL
40 mM 0.0306 mL 0.1532 mL 0.3065 mL 0.7662 mL
50 mM 0.0245 mL 0.1226 mL 0.2452 mL 0.6130 mL
60 mM 0.0204 mL 0.1022 mL 0.2043 mL 0.5108 mL
80 mM 0.0153 mL 0.0766 mL 0.1532 mL 0.3831 mL
100 mM 0.0123 mL 0.0613 mL 0.1226 mL 0.3065 mL
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Bizelesin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bizelesin129655-21-6NSC 615291 U-77779NSC615291NSC-615291U77779U 77779U-77779DNA Alkylator/CrosslinkerATDNA alkylatingreplicationanticancerInhibitorinhibitorinhibit

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