2. Protein Tyrosine Kinase/RTK
  3. GDNF Receptor
  4. BT18

BT18 is a molecule mimic with function similar to glial cell line-derived neurotrophic factor (GDNF). BT18 shows an effect on GDNF family receptor GFRα1 and RET receptor tyrosine kinase RetA function.

For research use only. We do not sell to patients.

BT18 Chemical Structure

BT18 Chemical Structure

CAS No. : 924811-53-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 140 In-stock
Solution
10 mM * 1 mL in DMSO USD 140 In-stock
Solid
5 mg USD 100 In-stock
10 mg USD 180 In-stock
50 mg USD 460 In-stock
100 mg USD 690 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

BT18 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BT18

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BT18 is a molecule mimic with function similar to glial cell line-derived neurotrophic factor (GDNF)[1]. BT18 shows an effect on GDNF family receptor GFRα1 and RET receptor tyrosine kinase RetA function[2].

In Vitro

BT18 (50 μM; 1-90 minutes) activates AKT and ERK1/2 in time-dependently in MG87RET cell lines[1].
BT18 (1-50 μM; 90 minutes) activates AKT in a dose-dependent manner in MG87RET cell lines[1].
BT18 (5-50 μM; 15 mimutes) appears to phosphorylate RET in concentration of 50 µM both in GFP-transfected and in GFRD1-transfected cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BT18 Related Antibodies

Western Blot Analysis[1]

Cell Line: MG87RET cell lines
Concentration: 50 μM
Incubation Time: 1, 5, 15, 45, 90 minutes
Result: Activated AKT and ERK1/2 in time-dependently.
Molecular Weight

637.64

Formula

C30H31F4N3O6S
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1CCN(C2=CC(S(=O)(N3CC4=C(C=C(OC)C(OC)=C4)CC3)=O)=CC=C2OC)CC1)C5=CC=C(F)C=C5C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (156.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5683 mL 7.8414 mL 15.6828 mL
5 mM 0.3137 mL 1.5683 mL 3.1366 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (3.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

SDS (252 KB)

COA (248 KB) HNMR (274 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5683 mL 7.8414 mL 15.6828 mL 39.2071 mL
5 mM 0.3137 mL 1.5683 mL 3.1366 mL 7.8414 mL
10 mM 0.1568 mL 0.7841 mL 1.5683 mL 3.9207 mL
15 mM 0.1046 mL 0.5228 mL 1.0455 mL 2.6138 mL
20 mM 0.0784 mL 0.3921 mL 0.7841 mL 1.9604 mL
25 mM 0.0627 mL 0.3137 mL 0.6273 mL 1.5683 mL
30 mM 0.0523 mL 0.2614 mL 0.5228 mL 1.3069 mL
40 mM 0.0392 mL 0.1960 mL 0.3921 mL 0.9802 mL
50 mM 0.0314 mL 0.1568 mL 0.3137 mL 0.7841 mL
60 mM 0.0261 mL 0.1307 mL 0.2614 mL 0.6535 mL
80 mM 0.0196 mL 0.0980 mL 0.1960 mL 0.4901 mL
100 mM 0.0157 mL 0.0784 mL 0.1568 mL 0.3921 mL
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BT18 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BT18924811-53-0BT 18BT-18GDNF ReceptorGlial-cell-line-derived neurotrophic factor receptorInhibitorinhibitorinhibit

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