2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. C18-Ceramide

C18-Ceramide is a bioactive molecule with multiple functions in cells, not a traditional agonist or inhibitor targeting a single site.

For research use only. We do not sell to patients.

C18-Ceramide Chemical Structure

C18-Ceramide Chemical Structure

CAS No. : 2304-81-6

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Based on 1 publication(s) in Google Scholar

Other Forms of C18-Ceramide:

C18-Ceramide Related Antibodies

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Description

C18-Ceramide is a bioactive molecule with multiple functions in cells, not a traditional agonist or inhibitor targeting a single site. It can act on multiple cellular targets, such as proteins related to endoplasmic reticulum stress (e.g., ATF-4, XBP-1, CHOP), proteins in the PI3K/AKT signaling pathway, and SNARE complex proteins. It exerts activities like inducing cell death, promoting autophagy, and regulating exocytosis through mechanisms such as activating endoplasmic reticulum stress, inhibiting the PI3K/AKT signaling pathway, and affecting lipid raft - related functions. It can be used in research on the mechanism of neuronal injury in the field of neuroscience and in the treatment research of cancers such as glioma in the field of oncology[1][2]. In Vitro:In the PC12 cell capacitance measurement experiment, C18-Ceramide (2 μM; transient) can significantly increase the cell membrane capacitance, indicating that it can promote exocytosis[1].
In the PC12 cell total internal reflection fluorescence microscopy (TIRFM) experiment, C18-Ceramide (2 μM) can rapidly reduce the number of NPY-EGFP-labeled subcellular membrane vesicles, which undergo endocytosis by fusing with the cell membrane[1].
In U251 and A172 glioma cells, C18-Ceramide (20 μM; 48 h) inhibits glioma cell viability and induces cell death[2].
C18-Ceramide (20 μM; 48 h) activates endoplasmic reticulum stress and increases the mRNA and protein levels of ATF-4, XBP-1 (s) and CHOP[2].
C18-Ceramide induced lethal autophagy, increased the punctate distribution and density of GFP-LC3, promoted the conversion of LC3B-I to LC3B-II and decreased the level of p62[2].
C18-Ceramide inhibited the PI3K/AKT pathway and decreased the expression levels of PI3K and p-AKT (S473)[2].
C18-Ceramide (20μM; co-treatment with VM-26 for 48h) increased the chemosensitivity of U251 and A172 cells to VM-26 (HY-13761), synergistically inhibited cell growth, and induced more apoptosis[2].
In Vivo:In the rat Kainate injury model, C18-Ceramide has an increasing content in the brain after injury. C18-Ceramide might facilitate the exocytosis of glutamate from damaged neurons, thus propagating neuronal injury[1].
In Vitro

C18-Ceramide Related Antibodies

Cell Viability Assay[2]

Cell Line: U251, A172 glioma cells
Concentration: 20 μM
Incubation Time: 48 h
Result: Inhibited ell viability and induced cell death.

RT-PCR[2]

Cell Line: U251, A172 glioma cells
Concentration: 20 μM
Incubation Time: 48 h
Result: Increased the mRNA and protein levels of ATF-4, XBP-1(s), and CHOP.

Western Blot Analysis[2]

Cell Line: U251, A172 glioma cells
Concentration: 20 μM
Incubation Time: 48 h
Result: Inhibited the expression levels of PI3K and p-AKT(S473).

Apoptosis Analysis[2]

Cell Line: U251, A172 glioma cells
Concentration: 20μM (C18-Ceramide), with 0.25 μM VM-26 for U251 cells, or 10 μM VM-26 for A172 cells
Incubation Time: 48 h
Result: Combined with VM-26, inhibited cell growth synergistically, and induced more apoptosis.
Molecular Weight

565.95

Formula

C36H71NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 10 mg/mL (17.67 mM; Need ultrasonic)

DMF : 1 mg/mL (1.77 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7669 mL 8.8347 mL 17.6694 mL
5 mM 0.3534 mL 1.7669 mL 3.5339 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / Ethanol 1 mM 1.7669 mL 8.8347 mL 17.6694 mL 44.1735 mL
Ethanol 5 mM 0.3534 mL 1.7669 mL 3.5339 mL 8.8347 mL
10 mM 0.1767 mL 0.8835 mL 1.7669 mL 4.4174 mL
15 mM 0.1178 mL 0.5890 mL 1.1780 mL 2.9449 mL
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C18-Ceramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C18-Ceramide2304-81-6Endogenous Metaboliteneurongliomacell deathInhibitorinhibitorinhibit

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