Chelidonine

Name: Chelidonine
Brand: MedChemExpress
SKU: HY-N2369
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2369 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Chelidonine, an isoquinoline alkaloid, can be isolated from Chelidonium majus L.. Chelidonine causes G_2/M arrest and induces caspase-dependent and caspase-independent apoptosis, and prevents cell cycle progression of stem cells in Dugesia japonica. Chelidonine has cytotoxic activity against melanoma cell lines. with anticancer and antiviral activity.

For research use only. We do not sell to patients.

Chelidonine Chemical Structure

CAS No. : 476-32-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 90	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 90	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 116	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Chelidonine:

1 Publications Citing Use of MCE Chelidonine

•Antiviral Res. 2024 Jul 2:228:105955. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	> 40 μM Compound: 1	Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
HepG2	IC₅₀	> 40 μM Compound: 1	Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
HL-60	IC₅₀	2.2 μM Compound: Chelidonine	Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay	[PMID: 34536668]
Jurkat	IC₅₀	2.2 μM Compound: Chelidonine	Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay	[PMID: 34536668]
K562	IC₅₀	17.65 μM Compound: 1	Antiproliferative activity against human K562 cells assessed as reduction in cell growth after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
L02	IC₅₀	> 40 μM Compound: 1	Antiproliferative activity against human L02 cells assessed as reduction in cell growth after 72 hrs by MTT assay Antiproliferative activity against human L02 cells assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
MCF7	IC₅₀	> 40 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
MOLT-4	IC₅₀	2.2 μM Compound: Chelidonine	Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay	[PMID: 34536668]
PBMC	IC₅₀	> 40 μM Compound: 1	Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 31883693]
RAW264.7	IC₅₀	7.3 μM Compound: 5	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay	[PMID: 22024033]

In Vitro

Chelidonine (5, 10 and 20 μM; 3-4 days) causes lesions and ventral curling in Dugesia japonica; and significantly decreases Djmcm2 expression at 20 μM but no reduction is observed at 5 and 10 μM; as well as prevents cell cycle progression of stem cells^[2].
Chelidonine (0-3 μg/mL; 48 h) has cytotoxic activity against melanoma cell lines^[3].
Chelidonine (1, 2 and 3 μg/mL; 24 h) decreases mitochondrial membrane potential (MMP) in 50% of A-375 cells at 1 and 1.5 μg/mL, and 62% at 3 μg/mL^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[3]

Cell Line:	A-375, A-375-p53DD and A-375-p53sh
Concentration:	0-3 μg/mL
Incubation Time:	48 h
Result:	Exhibited cytotoxic activity against melanoma cell lines with 0.910±0.017 μg/ml, 0.634±0.009 μg/ml and 0.772±0.045 μg/ml in A-375, A-375-p53DD and A-375-p53sh, respectively.

Molecular Weight

353.37

Formula

C₂₀H₁₉NO₅

CAS No.

476-32-4

Appearance

Solid

Color

White to off-white

SMILES

CN1[C@@]2([H])[C@@]([C@H](CC3=C2C=C4C(OCO4)=C3)O)([H])C5=C(C6=C(OCO6)C=C5)C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (282.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8299 mL	14.1495 mL	28.2990 mL
5 mM	0.5660 mL	2.8299 mL	5.6598 mL
10 mM	0.2830 mL	1.4149 mL	2.8299 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (277 KB) SDS (392 KB)

COA (251 KB) HNMR (316 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Lee YK, et al. Chelidonine Induces Caspase-Dependent and Caspase-Independent Cell Death through G2/M Arrest in the T98G Human Glioblastoma Cell Line. Evid Based Complement Alternat Med. 2019 May 26;2019:6318179. [Content Brief]

[2]. Isolani ME, et al. The in vivo effect of chelidonine on the stem cell system of planarians. Eur J Pharmacol. 2012 Jul 5;686(1-3):1-7. [Content Brief]

[3]. Hammerová J, et al. Benzo[c]phenanthridine alkaloids exhibit strong anti-proliferative activity in malignant melanoma cells regardless of their p53 status. J Dermatol Sci. 2011 Apr;62(1):22-35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8299 mL	14.1495 mL	28.2990 mL	70.7474 mL
	5 mM	0.5660 mL	2.8299 mL	5.6598 mL	14.1495 mL
	10 mM	0.2830 mL	1.4149 mL	2.8299 mL	7.0747 mL
	15 mM	0.1887 mL	0.9433 mL	1.8866 mL	4.7165 mL
	20 mM	0.1415 mL	0.7075 mL	1.4149 mL	3.5374 mL
	25 mM	0.1132 mL	0.5660 mL	1.1320 mL	2.8299 mL
	30 mM	0.0943 mL	0.4716 mL	0.9433 mL	2.3582 mL
	40 mM	0.0707 mL	0.3537 mL	0.7075 mL	1.7687 mL
	50 mM	0.0566 mL	0.2830 mL	0.5660 mL	1.4149 mL
	60 mM	0.0472 mL	0.2358 mL	0.4716 mL	1.1791 mL
	80 mM	0.0354 mL	0.1769 mL	0.3537 mL	0.8843 mL
	100 mM	0.0283 mL	0.1415 mL	0.2830 mL	0.7075 mL

Chelidonine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chelidonine476-32-4ApoptosisInfluenza Virusisoquinoline alkaloidanticancerantiviralmelanoma cellDugesia japonicaA-375Inhibitorinhibitorinhibit

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Chelidonine

Customer Review

Other Forms of Chelidonine:

Chelidonine Related Antibodies

1 Publications Citing Use of MCE Chelidonine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Chelidonine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Chelidonine Related Classifications

Keywords:

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