Chlorin e6 (Synonyms: Ce6)

Name: Chlorin e6
Brand: MedChemExpress
SKU: HY-13594
Rating: 5.0 (6 reviews)

Cat. No.: HY-13594 Purity: 98.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer.

For research use only. We do not sell to patients.

Chlorin e6 Chemical Structure

CAS No. : 19660-77-6

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE Chlorin e6

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•Related Small Molecules:

•Related Proteins:

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View All Isoforms

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All PARP Isoform Specific Products:

View All Isoforms

PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Leishmania

Bax

Bcl-2

Caspase 3

PARP-1

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 50 μM Compound: Ce6	Dark toxicity in human HeLa cells assessed as reduction in cell viability after 3 hrs by MTT assay Dark toxicity in human HeLa cells assessed as reduction in cell viability after 3 hrs by MTT assay	[PMID: 32787080]
HeLa	IC₅₀	1.92 μM Compound: Ce6	Phototoxicity against human HeLa cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay Phototoxicity against human HeLa cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay	[PMID: 32787080]
HEp-2	IC₅₀	> 400 μM Compound: 5	Cytotoxicity against human Hep2 cells under dark condition after 18 hrs by celltiter blue assay Cytotoxicity against human Hep2 cells under dark condition after 18 hrs by celltiter blue assay	[PMID: 21936519]
HEp-2	IC₅₀	20.8 μM Compound: 5	Cytotoxicity against human Hep2 cells after overnight incubation followed by cells irradiated with 1 J/cm'2 light for 20 mins measured after overnight incubation by celltiter blue assay Cytotoxicity against human Hep2 cells after overnight incubation followed by cells irradiated with 1 J/cm'2 light for 20 mins measured after overnight incubation by celltiter blue assay	[PMID: 21936519]
HepG2	IC₅₀	41.2 μM Compound: Chlorin e6	Photo cytotoxic activity against human HepG2 cells irradiated with 1.7 J/cm'2 light dose for 10 mins measured after overnight incubation by MTT assay Photo cytotoxic activity against human HepG2 cells irradiated with 1.7 J/cm'2 light dose for 10 mins measured after overnight incubation by MTT assay	[PMID: 26306981]
HepG2	IC₅₀	52.9 μM Compound: Chlorin e6	Dark toxicity in human HepG2 cells after overnight incubation by MTT assay Dark toxicity in human HepG2 cells after overnight incubation by MTT assay	[PMID: 26306981]
MCF7	IC₅₀	> 50 μM Compound: Ce6	Dark toxicity in human MCF7 cells assessed as reduction in cell viability after 3 hrs by MTT assay Dark toxicity in human MCF7 cells assessed as reduction in cell viability after 3 hrs by MTT assay	[PMID: 32787080]
MCF7	IC₅₀	3.4 μM Compound: Ce6	Phototoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay Phototoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay	[PMID: 32787080]
SK-OV-3	IC₅₀	0.39 μM Compound: Chlorine E6	Photocytotoxicity in human SKOV3 cells assessed as cell death incubated for 6 hrs followed by PDT-1200 light irradiation at 20 J/cm2 measured after 16 hrs using liposome-encapsulated compound by MTT assay Photocytotoxicity in human SKOV3 cells assessed as cell death incubated for 6 hrs followed by PDT-1200 light irradiation at 20 J/cm2 measured after 16 hrs using liposome-encapsulated compound by MTT assay	[PMID: 29670694]
SW480	IC₅₀	> 50 μM Compound: Ce6	Dark toxicity in human SW480 cells assessed as reduction in cell viability after 3 hrs by MTT assay Dark toxicity in human SW480 cells assessed as reduction in cell viability after 3 hrs by MTT assay	[PMID: 32787080]
SW480	IC₅₀	3.1 μM Compound: Ce6	Phototoxicity against human SW480 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay Phototoxicity against human SW480 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay	[PMID: 32787080]

In Vitro

Chlorin e6 (100 μg/mL, 24 h) with laser light (635 nm) exhibits no toxicity viability in HT-29 cells^[2].
Chlorin e6 (5-10 μM, 3 h) with near-infrared laser light (660 nm) induces pancreatic cancer cell apoptosis via caspase-3 activation, resulting in tumor growth suppression in AsPC-1 and MIA PaCa-2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	AsPC-1 and MIA PaCa-2 Cells
Concentration:	5 μM, 10 μM
Incubation Time:	3 h
Result:	Significantly reduced Bcl-2 protein expression while increasing apoptotic molecule expression of Bax. Activated caspase-3 through its cleavage and upregulated the expression of cleaved PARP-1 in a dose-dependent manner.

In Vivo

Chlorin e6 (2.5 mg/kg, Intravenous injection, single dose) with a red light at a rate of 100 J/cm² for 8 min 20 s has anti-tumor activity in B16F10 melanoma cells and PANC02 Pancreatic Cells xenograft mouse model^[3].
Chlorin e6 (2.5 mg/kg, Intravenous injection, single dose) with near-infrared laser light (660 nm) suppresses the tumor growth in canine tumors^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	B16F10 melanoma cells xenograft mouse, PANC02 pancreatic cells xenograft mouse^[3]
Dosage:	2.5 mg/kg
Administration:	Intravenous injection (i.v.)
Result:	Significantly reduced tumor volume.

Molecular Weight

596.67

Formula

C₃₄H₃₆N₄O₆

CAS No.

19660-77-6

Appearance

Solid

Color

Brown to black

SMILES

OC(C/C1=C2[C@H]([C@@H](C(/C=C3N/C(C(C=C)=C\3C)=C\C4=N/C(C(CC)=C4C)=C\C5=C(C)C(C(O)=O)=C1N5)=N\2)C)CCC(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (33.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6760 mL	8.3798 mL	16.7597 mL
5 mM	0.3352 mL	1.6760 mL	3.3519 mL
10 mM	0.1676 mL	0.8380 mL	1.6760 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (3.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.31%

Select Batch:

Data Sheet (288 KB) SDS (393 KB)

COA (264 KB) HNMR (149 KB) RP-HPLC (141 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Nie M, et al. Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm [J]. Photodiagnosis and photodynamic therapy, 2020, 31: 101817. [Content Brief]

[2]. Karuppusamy S, et al. Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer [J]. Colloids and Surfaces B: Biointerfaces, 2019, 181: 778-788. [Content Brief]

[3]. Shrestha R, et al. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors [J]. Life, 2022, 12(12): 2102. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6760 mL	8.3798 mL	16.7597 mL	41.8992 mL
	5 mM	0.3352 mL	1.6760 mL	3.3519 mL	8.3798 mL
	10 mM	0.1676 mL	0.8380 mL	1.6760 mL	4.1899 mL
	15 mM	0.1117 mL	0.5587 mL	1.1173 mL	2.7933 mL
	20 mM	0.0838 mL	0.4190 mL	0.8380 mL	2.0950 mL
	25 mM	0.0670 mL	0.3352 mL	0.6704 mL	1.6760 mL
	30 mM	0.0559 mL	0.2793 mL	0.5587 mL	1.3966 mL

Chlorin e6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Chlorin e6 (Synonyms: Ce6)

Customer Review

Chlorin e6 Related Antibodies

6 Publications Citing Use of MCE Chlorin e6

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Bcl-2 Family Isoform Specific Products:

View All Caspase Isoform Specific Products:

View All PARP Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Chlorin e6 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Chlorin e6 Related Classifications

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