Ciwujianoside C3

Name: Ciwujianoside C3
Brand: MedChemExpress
SKU: HY-N4134
Rating: 4.5 (1 reviews)

Cat. No.: HY-N4134 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ciwujianoside C3, an orally active and brain penetrated compound, is isolated the leaves of Acanthopanax henryi Harms. Ciwujianoside C3 has anti-inflammatory effect and can reinforces object recognition memory.

For research use only. We do not sell to patients.

Ciwujianoside C3 Chemical Structure

CAS No. : 114906-74-0

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Cell Viability Assay^[1]

Cell Line:	RAW 264.7 cells
Concentration:	10 µM, 20 µM and 40 µM
Incubation Time:	24 hours
Result:	Has no effect on cell cytotoxicity and inhibits NO production

Western Blot Analysis^[1]

Cell Line:	RAW 264.7 cells
Concentration:	10 µM, 20 µM and 40 µM
Incubation Time:	24 hours
Result:	Decreases p-ERK and p-JNK expression.

RT-PCR^[1]

Cell Line:	RAW 264.7 cells
Concentration:	10 µM, 20 µM and 40 µM
Incubation Time:	24 hours
Result:	Decreased COX-2 and iNOS mRNA expression in a dose-dependent manner.

In Vivo

Animal Model:	Mice^[1]
Dosage:	0.5 mg/kg
Administration:	Oral administration; 0.5 mg/kg/day; 17 days
Result:	Reinforced object recognition memory.

Molecular Weight

1059.24

Formula

C₅₃H₈₆O₂₁

CAS No.

114906-74-0

Appearance

Solid

Color

White to off-white

SMILES

OC[C@H]([C@@H](O[C@@]1([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O1)O)O)[C@H](O)[C@H]2O)O[C@H]2OC[C@H]([C@@H](O)[C@H](O)[C@H]3O)O[C@H]3OC([C@]45[C@](CC(C)(C)CC5)([H])C6=CC[C@@]([C@@]7([C@@](C(C)([C@@H](O[C@@]8([H])[C@@H]([C@H]([C@@H](O)CO8)O)O)CC7)C)([H])CC9)C)([H])[C@]9(C)[C@]6(C)CC4)=O

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (47.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9441 mL	4.7204 mL	9.4407 mL
5 mM	0.1888 mL	0.9441 mL	1.8881 mL
10 mM	0.0944 mL	0.4720 mL	0.9441 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (1.18 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (258 KB)

Handling Instructions (2659 KB)

References

[1]. Kang DH, et al. Anti-inflammatory effects of Ciwujianoside C3, extracted from the leaves of Acanthopanax henryi (Oliv.) Harms, on LPS-stimulated RAW 264.7 cells. Mol Med Rep. 2016 Oct;14(4):3749-58. [Content Brief]

[2]. Yamauchi Y, et al. Memory Enhancement by Oral Administration of Extract of Eleutherococcus senticosus Leaves and Active Compounds Transferred in the Brain.Nutrients. 2019 May 22;11(5). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9441 mL	4.7204 mL	9.4407 mL	23.6018 mL
	5 mM	0.1888 mL	0.9441 mL	1.8881 mL	4.7204 mL
	10 mM	0.0944 mL	0.4720 mL	0.9441 mL	2.3602 mL
	15 mM	0.0629 mL	0.3147 mL	0.6294 mL	1.5735 mL
	20 mM	0.0472 mL	0.2360 mL	0.4720 mL	1.1801 mL
	25 mM	0.0378 mL	0.1888 mL	0.3776 mL	0.9441 mL
	30 mM	0.0315 mL	0.1573 mL	0.3147 mL	0.7867 mL
	40 mM	0.0236 mL	0.1180 mL	0.2360 mL	0.5900 mL