2. Neuronal Signaling
  3. Serotonin Transporter
  4. Clomipramine hydrochloride

Clomipramine hydrochloride  (Synonyms: Chlorimipramine hydrochloride; G-34586 hydrochloride; NSC-169865 hydrochloride)

Cat. No.: HY-B0457 Purity: 99.14%
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Clomipramine (Chlorimipramine) hydrochloride is a potent 5-HT reuptake blocker with the IC50 value of 1.5 nM. Clomipramine hydrochloride is a tricyclic antidepressant that can be used for the research of depression and obsessive compulsive disorder (OCD).

For research use only. We do not sell to patients.

Clomipramine hydrochloride Chemical Structure

Clomipramine hydrochloride Chemical Structure

CAS No. : 17321-77-6

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5 g USD 98 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Clomipramine hydrochloride:

Clomipramine hydrochloride Related Antibodies

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Description

Clomipramine (Chlorimipramine) hydrochloride is a potent 5-HT reuptake blocker with the IC50 value of 1.5 nM. Clomipramine hydrochloride is a tricyclic antidepressant that can be used for the research of depression and obsessive compulsive disorder (OCD)[1].

In Vitro

Clomipramine hydrochloride can inhibit reuptake of both noradrenaline and 5-HT, although Clomipramine inhibits 5-HT reuptake more strongly than it inhibits noradrenaline reuptake[1].
The antidepressant Clomipramine inhibits both venom AChE as well as human serum BChE in a concentration-dependent manner but has no effect on AChE in the rat brain striatum[2].
Clomipramine hydrochloride interferes with the autophagic flux and severely compromises the viability of tumorigenic cells upon cytotoxic stress[3].
Clomipramine hydrochloride reduces autophagy in neuronal primary cultures. Clomipramine (1 and 5 μM) hydrochloride negatively regulates neuronal autophagic pathway in primary cultured cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clomipramine hydrochloride Related Antibodies

Western Blot Analysis[3]

Cell Line: Primary cortical neurons
Concentration: 1 and 5 µM
Incubation Time: 12, 24 and 48 hours
Result: Enhanced the LC3-I conversion to LC3-II in a concentration-dependent manner at all analyzed time points.
In Vivo

Clomipramine (5-20 mg/kg; i.p) hydrochloride elicits significant hyperglycemia in mice. Clomipramine hydrochloride induces hyperglycemia in mice by blocking the 5-HT2B and/or 5-HT2C receptors, which results in facilitation of adrenaline release. In mice, Clomipramine hydrochloride reduces immobility in the forced swimming test, which is the behavioral model for antidepressants. Clomipramine hydrochloride also inhibits the OCD animal model, marble burying behavior in mice[1].
Clomipramine (20 mg/kg) hydrochloride decreases autophagic flux in murine tissues[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 J mice (6 weeks of age and 22 to 25 g)[3]
Dosage: 20 mg/kg
Administration: Treated intraperitoneally for 21 days
Result: Both LC3-II and p62 were significantly increased in the liver of Clomipramine treated mice compared to vehicle treated ones.
Clinical Trial
Molecular Weight

351.31

Formula

C19H24Cl2N2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CN(C)CCCN1C2=CC(Cl)=CC=C2CCC3=C1C=CC=C3.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (142.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 50 mg/mL (142.32 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8465 mL 14.2324 mL 28.4649 mL
5 mM 0.5693 mL 2.8465 mL 5.6930 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3 mg/mL (8.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3 mg/mL (8.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (284.65 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.8465 mL 14.2324 mL 28.4649 mL 71.1622 mL
5 mM 0.5693 mL 2.8465 mL 5.6930 mL 14.2324 mL
10 mM 0.2846 mL 1.4232 mL 2.8465 mL 7.1162 mL
15 mM 0.1898 mL 0.9488 mL 1.8977 mL 4.7441 mL
20 mM 0.1423 mL 0.7116 mL 1.4232 mL 3.5581 mL
25 mM 0.1139 mL 0.5693 mL 1.1386 mL 2.8465 mL
30 mM 0.0949 mL 0.4744 mL 0.9488 mL 2.3721 mL
40 mM 0.0712 mL 0.3558 mL 0.7116 mL 1.7791 mL
50 mM 0.0569 mL 0.2846 mL 0.5693 mL 1.4232 mL
60 mM 0.0474 mL 0.2372 mL 0.4744 mL 1.1860 mL
80 mM 0.0356 mL 0.1779 mL 0.3558 mL 0.8895 mL
100 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7116 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Clomipramine hydrochloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Clomipramine17321-77-6ChlorimipramineG-34586NSC-169865G34586G 34586NSC169865NSC 169865Serotonin Transporter5-HTTSERTSLC6A4Antidepressant5-hydroxytryptamine5-HThyperglycemiaantidepressant5-HT reuptakedepressionobsessive compulsive disorder,OCDInhibitorinhibitorinhibit

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