Clomipramine (Synonyms: Chlorimipramine; G-34586; NSC-169865)

Name: Clomipramine
Brand: MedChemExpress
SKU: HY-B0457A
Rating: 5.0 (2 reviews)

Cat. No.: HY-B0457A Purity: 99.48%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Clomipramine (Chlorimipramine) is a potent 5-HT reuptake blocker with the IC₅₀ value of 1.5 nM. Clomipramine is a tricyclic antidepressant that can be used for the research of depression and obsessive compulsive disorder (OCD).

For research use only. We do not sell to patients.

Clomipramine Chemical Structure

CAS No. : 303-49-1

Size	Price	Stock	Quantity
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 2 publication(s) in Google Scholar

Other Forms of Clomipramine:

2 Publications Citing Use of MCE Clomipramine

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	19.3 μM Compound: clomipramine	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]
NCI-H1650	GI₅₀	> 40 μM Compound: Clomipramine	Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26372073]
Ventricular myocyte	IC₅₀	11.6 μM Compound: Clomipramine	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]

In Vitro

Clomipramine can inhibit reuptake of both noradrenaline and 5-HT, although Clomipramine inhibits 5-HT reuptake more strongly than it inhibits noradrenaline reuptake^[1].
The antidepressant Clomipramine inhibits both venom AChE as well as human serum BChE in a concentration-dependent manner but has no effect on AChE in the rat brain striatum^[2]. Clomipramine interferes with the autophagic flux and severely compromises the viability of tumorigenic cells upon cytotoxic stress^[3].
Clomipramine reduces autophagy in neuronal primary cultures. Clomipramine (1 and 5 µM) negatively regulates neuronal autophagic pathway in primary cultured cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Primary cortical neurons
Concentration:	1 and 5 µM
Incubation Time:	12, 24 and 48 hours
Result:	Enhanced the LC3-I conversion to LC3-II in a concentration-dependent manner at all analyzed time points.

In Vivo

Clomipramine (5-20 mg/kg; i.p) elicits significant hyperglycemia in mice. Clomipramine induces hyperglycemia in mice by blocking the 5-HT2B and/or 5-HT2C receptors, which results in facilitation of adrenaline release. In mice, Clomipramine reduces immobility in the forced swimming test, which is the behavioral model for antidepressants. Clomipramine also inhibits the OCD animal model, marble burying behavior in mice^[1]. Clomipramine (20 mg/kg) decreases autophagic flux in murine tissues^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 J mice (6 weeks of age and 22 to 25 g)^[3]
Dosage:	20 mg/kg
Administration:	Treated intraperitoneally for 21 days
Result:	Both LC3-II and p62 were significantly increased in the liver of Clomipramine treated mice compared to vehicle treated ones.

Clinical Trial

Molecular Weight

314.85

Formula

C₁₉H₂₃ClN₂

CAS No.

303-49-1

Appearance

Oil

Color

Off-white to light yellow

SMILES

CN(C)CCCN1C2=CC(Cl)=CC=C2CCC3=CC=CC=C31

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (317.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1761 mL	15.8806 mL	31.7612 mL
5 mM	0.6352 mL	3.1761 mL	6.3522 mL
10 mM	0.3176 mL	1.5881 mL	3.1761 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (255 KB) HNMR (253 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Yumi Sugimoto, et al. The tricyclic antidepressant Clomipramine increases plasma glucose levels of mice. J Pharmacol Sci. 2003 Sep;93(1):74-9. [Content Brief]

[2]. Mushtaq Ahmed, et al. Comparative study of the inhibitory effect of antidepressants on cholinesterase activity in Bungarus sindanus (krait) venom, human serum and rat striatum. J Enzyme Inhib Med Chem. 2008 Dec;23(6):912-7. [Content Brief]

[3]. Federica Cavaliere,The tricyclic antidepressant Clomipramine inhibits neuronal autophagic flux. Sci Rep. 2019 Mar 19;9(1):4881. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1761 mL	15.8806 mL	31.7612 mL	79.4029 mL
	5 mM	0.6352 mL	3.1761 mL	6.3522 mL	15.8806 mL
	10 mM	0.3176 mL	1.5881 mL	3.1761 mL	7.9403 mL
	15 mM	0.2117 mL	1.0587 mL	2.1174 mL	5.2935 mL
	20 mM	0.1588 mL	0.7940 mL	1.5881 mL	3.9701 mL
	25 mM	0.1270 mL	0.6352 mL	1.2704 mL	3.1761 mL
	30 mM	0.1059 mL	0.5294 mL	1.0587 mL	2.6468 mL
	40 mM	0.0794 mL	0.3970 mL	0.7940 mL	1.9851 mL
	50 mM	0.0635 mL	0.3176 mL	0.6352 mL	1.5881 mL
	60 mM	0.0529 mL	0.2647 mL	0.5294 mL	1.3234 mL
	80 mM	0.0397 mL	0.1985 mL	0.3970 mL	0.9925 mL
	100 mM	0.0318 mL	0.1588 mL	0.3176 mL	0.7940 mL

Clomipramine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Clomipramine (Synonyms: Chlorimipramine; G-34586; NSC-169865)

Customer Review

Other Forms of Clomipramine:

Clomipramine Related Antibodies

2 Publications Citing Use of MCE Clomipramine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Clomipramine Related Antibodies

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Complete Stock Solution Preparation Table

Clomipramine Related Classifications

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