2. Membrane Transporter/Ion Channel
  3. Sodium Channel
  4. Clopamide

Clopamide is an orally active thiazide-like diuretic agent that inhibits the sodium-coupled chloride cotransporter SLC12A3. Clopamide has the potential for hypertension and cardiac failure research.

For research use only. We do not sell to patients.

Clopamide Chemical Structure

Clopamide Chemical Structure

CAS No. : 636-54-4

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Clopamide:

Clopamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Clopamide

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Clopamide is an orally active thiazide-like diuretic agent that inhibits the sodium-coupled chloride cotransporter SLC12A3. Clopamide has the potential for hypertension and cardiac failure research[1][2].

In Vitro

Clopamide is actively secreted by renal tubular cells and the True Tubular Excretion Fraction (TTEF) value is 10%[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clopamide Related Antibodies
In Vivo

The venoconstrictor response to bradykinin is attenuated after oral administration of Clopamide (0.5 mg/kg), and by concomitant local infusion of cyclosporine-A (1-10 μg/min) in conscious dogs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

345.84

Formula

C14H20ClN3O3S
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(NN1[C@H](C)CCC[C@@H]1C)C2=CC=C(Cl)C(S(=O)(N)=O)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (289.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8915 mL 14.4576 mL 28.9151 mL
5 mM 0.5783 mL 2.8915 mL 5.7830 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8915 mL 14.4576 mL 28.9151 mL 72.2878 mL
5 mM 0.5783 mL 2.8915 mL 5.7830 mL 14.4576 mL
10 mM 0.2892 mL 1.4458 mL 2.8915 mL 7.2288 mL
15 mM 0.1928 mL 0.9638 mL 1.9277 mL 4.8192 mL
20 mM 0.1446 mL 0.7229 mL 1.4458 mL 3.6144 mL
25 mM 0.1157 mL 0.5783 mL 1.1566 mL 2.8915 mL
30 mM 0.0964 mL 0.4819 mL 0.9638 mL 2.4096 mL
40 mM 0.0723 mL 0.3614 mL 0.7229 mL 1.8072 mL
50 mM 0.0578 mL 0.2892 mL 0.5783 mL 1.4458 mL
60 mM 0.0482 mL 0.2410 mL 0.4819 mL 1.2048 mL
80 mM 0.0361 mL 0.1807 mL 0.3614 mL 0.9036 mL
100 mM 0.0289 mL 0.1446 mL 0.2892 mL 0.7229 mL
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Clopamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Clopamide636-54-4Sodium ChannelNa channelsNa+ channelsSLC12A3thiazide-likediureticantihypertensivecardiacfailureInhibitorinhibitorinhibit

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