CNDAC hydrochloride

Name: CNDAC hydrochloride
Brand: MedChemExpress
SKU: HY-16445B
Rating: 4.5 (1 reviews)

Cat. No.: HY-16445B Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

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CNDAC hydrochloride is a metabolite of the orally active agent Sapacitabine (HY-16445), and a nucleoside analog. CNDAC hydrochloride induces DNA damage and apoptosis.

For research use only. We do not sell to patients.

CNDAC hydrochloride Chemical Structure

CAS No. : 134665-72-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 385	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 385	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1000	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1600	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of CNDAC hydrochloride:

CNDAC Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

CNDAC hydrochloride is a metabolite of the orally active agent Sapacitabine (HY-16445), and a nucleoside analog. CNDAC hydrochloride induces DNA damage and apoptosis^[1]^[2].

In Vitro

CNDAC has a unique mechanism of action: after incorporation into DNA, it induces single-strand breaks (SSBs) that are converted into double-strand breaks (DSBs) when cells go through a second S phase^[1].
Lack of Rad51D and XRCC3 sensitizes cells to CNDAC (0-1 μM; 24 h)^[1].
CNDAC (0-100 μM; 3 days) inhibits proliferation of HL-60 and THP-1 cells^[2].
CNDAC (0-10 μM; 3-6 days) induces apoptosis in HL-60 and THP-1 cells^[2].
CNDAC (6 μM; 48 h) induces cell cycle arrest in the G2 phase following a delayed S phase in HCT116 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Rad51D-deficient 51D1, Rad51D-complemented 51D1.3, wild-type AA8 and XRCC3-deficient irs1SF CHO cells
Concentration:	0-1 μM
Incubation Time:	24 h
Result:	Inhibited cell survival with IC₅₀s of 0.006, 0.32, 0.48 and 0.0053 μM against Rad51D-deficient 51D1, Rad51D-complemented 51D1.3, wild-type AA8 and XRCC3-deficient irs1SF cell lines, respectively.

Cell Proliferation Assay^[2]

Cell Line:	HL-60 and THP-1 cells
Concentration:	0-100 μM
Incubation Time:	3 days
Result:	Inhibited proliferation with IC₅₀s of 1.5832 μM and 0.84 μM against HL-60 and THP-1 cells, respectively.

Apoptosis Analysis^[2]

Cell Line:	HL-60 and THP-1 cells
Concentration:	0, 0.5, 1, 2, 3, 4, 5 and 10 μM
Incubation Time:	3, 4, 5, and 6 days
Result:	Induced apoptosis in both cells.

Cell Cycle Analysis^[3]

Cell Line:	HCT116
Concentration:	6 μM
Incubation Time:	48 h
Result:	36 and 36% of cells were arrested in late-S and G2/M phases, respectively.

In Vivo

CNDAC (20mg/kg; i.p.; daily for 10 days) shows antitumor activity in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CDF1 mice, P388 tumor model^[4]
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection, daily for 10 days
Result:	Greatly increased the survival time and survival rate.

Molecular Weight

288.69

Formula

C₁₀H₁₃ClN₄O₄

CAS No.

134665-72-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(N=C(N)C=C1)N1[C@H]2[C@@H](C#N)[C@H](O)[C@@H](CO)O2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (432.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4639 mL	17.3196 mL	34.6392 mL
5 mM	0.6928 mL	3.4639 mL	6.9278 mL
10 mM	0.3464 mL	1.7320 mL	3.4639 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (251 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Liu XJ, et al. Sapacitabine, the prodrug of CNDAC, is a nucleoside analog with a unique action mechanism of inducing DNA strand breaks. Chin J Cancer. 2012 Aug;31(8):373-80. [Content Brief]

[2]. Jagan S, et al. Bone Marrow and Peripheral Blood AML Cells Are Highly Sensitive to CNDAC, the Active Form of Sapacitabine. Adv Hematol. 2012;2012:727683. [Content Brief]

[3]. Serova M, et al. Antiproliferative effects of sapacitabine (CYC682), a novel 2'-deoxycytidine-derivative, in human cancer cells. Br J Cancer. 2007 Sep 3;97(5):628-36. [Content Brief]

[4]. Azuma A, et al. Nucleosides and nucleotides. 122. 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosylcytosine and its derivatives. A new class of nucleoside with a broad antitumor spectrum. J Med Chem. 1993 Dec 24;36(26):4183-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4639 mL	17.3196 mL	34.6392 mL	86.5981 mL
	5 mM	0.6928 mL	3.4639 mL	6.9278 mL	17.3196 mL
	10 mM	0.3464 mL	1.7320 mL	3.4639 mL	8.6598 mL
	15 mM	0.2309 mL	1.1546 mL	2.3093 mL	5.7732 mL
	20 mM	0.1732 mL	0.8660 mL	1.7320 mL	4.3299 mL
	25 mM	0.1386 mL	0.6928 mL	1.3856 mL	3.4639 mL
	30 mM	0.1155 mL	0.5773 mL	1.1546 mL	2.8866 mL
	40 mM	0.0866 mL	0.4330 mL	0.8660 mL	2.1650 mL
	50 mM	0.0693 mL	0.3464 mL	0.6928 mL	1.7320 mL
	60 mM	0.0577 mL	0.2887 mL	0.5773 mL	1.4433 mL
	80 mM	0.0433 mL	0.2165 mL	0.4330 mL	1.0825 mL
	100 mM	0.0346 mL	0.1732 mL	0.3464 mL	0.8660 mL