Coproporphyrin I

Cat. No.: HY-113318: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Coproporphyrin I is an endogenous metabolite present in Urine and Blood that can be used for the research of Liver Disease and Porphyria.

For research use only. We do not sell to patients.

Coproporphyrin I Chemical Structure

CAS No. : 531-14-6

Size	Price	Stock
1 mg	USD 160	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

Other Forms of Coproporphyrin I:

Coproporphyrin I dihydrochloride Get quote

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Biological Activity
Purity & Documentation
References
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Description

Coproporphyrin I is an endogenous metabolite present in Urine and Blood that can be used for the research of Liver Disease and Porphyria^[1]^[2]^[3]^[4].

In Vitro

Endogenous metabolites is defined as those that are annotated by Kyoto Encyclopedia of Genes and Genomes as substrates or products of the ~1900 metabolic enzymes encoded in our genome. It is clear in the body of literature that there are documented toxic properties for many of these metabolites^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

654.71

Formula

C₃₆H₃₈N₄O₈

CAS No.

531-14-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCC1=C2/C=C3C(C)=C(CCC(O)=O)C(/C=C(N/4)/C(C)=C(CCC(O)=O)C4=C\C5=N/C(C(CCC(O)=O)=C5C)=C\C(N2)=C1C)=N/3

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (76.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5274 mL	7.6370 mL	15.2739 mL
5 mM	0.3055 mL	1.5274 mL	3.0548 mL
10 mM	0.1527 mL	0.7637 mL	1.5274 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.62 mg/mL (0.95 mM); Clear solution

This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥85.0%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (255 KB)

Handling Instructions (2659 KB)

References

[1]. Lee N, et al. Endogenous toxic metabolites and implications in cancer therapy. Oncogene. 2020 Aug;39(35):5709-5720. [Content Brief]

[2]. Zuijderhoudt FM, et al. On accuracy and precision of a HPLC method for measurement of urine porphyrin concentrations. Clin Chem Lab Med. 2000 Mar;38(3):227-30. [Content Brief]

[3]. Hindmarsh JT, et al. Biochemical differentiation of the porphyrias. Clin Biochem. 1999 Nov;32(8):609-19. [Content Brief]

[4]. MAGNUS IA, et al. Erythropoietic protoporphyria. A new porphyria syndrome with solar urticaria due to protoporphyrinaemia. Lancet. 1961 Aug 26;2(7200):448-51. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5274 mL	7.6370 mL	15.2739 mL	38.1848 mL
	5 mM	0.3055 mL	1.5274 mL	3.0548 mL	7.6370 mL
	10 mM	0.1527 mL	0.7637 mL	1.5274 mL	3.8185 mL
	15 mM	0.1018 mL	0.5091 mL	1.0183 mL	2.5457 mL
	20 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9092 mL
	25 mM	0.0611 mL	0.3055 mL	0.6110 mL	1.5274 mL
	30 mM	0.0509 mL	0.2546 mL	0.5091 mL	1.2728 mL
	40 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	50 mM	0.0305 mL	0.1527 mL	0.3055 mL	0.7637 mL
	60 mM	0.0255 mL	0.1273 mL	0.2546 mL	0.6364 mL