2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. Cyanine 5 Tyramide

Cyanine 5 Tyramide  (Synonyms: Tyramide-Cy5)

Cat. No.: HY-136247 Purity: 95.17%
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Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.

For research use only. We do not sell to patients.

Cyanine 5 Tyramide Chemical Structure

Cyanine 5 Tyramide Chemical Structure

CAS No. : 1431148-26-3

Size Price Stock Quantity
1 mg USD 210 In-stock
5 mg USD 620 In-stock
10 mg USD 980 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyanine 5 Tyramide:

Cyanine 5 Tyramide Related Antibodies

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Description

Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1].

Molecular Weight

775.97

Formula

C41H49N3O8S2
CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

670
Excitation (Ex)

645
SMILES

O=S(C1=CC2=C([N+](CC)=C(/C=C/C=C/C=C3N(CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(S(=O)(O)=O)C=C5)C/3(C)C)C2(C)C)C=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (80.54 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2887 mL 6.4435 mL 12.8871 mL
5 mM 0.2577 mL 1.2887 mL 2.5774 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (2.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (2.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2887 mL 6.4435 mL 12.8871 mL 32.2177 mL
5 mM 0.2577 mL 1.2887 mL 2.5774 mL 6.4435 mL
10 mM 0.1289 mL 0.6444 mL 1.2887 mL 3.2218 mL
15 mM 0.0859 mL 0.4296 mL 0.8591 mL 2.1478 mL
20 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6109 mL
25 mM 0.0515 mL 0.2577 mL 0.5155 mL 1.2887 mL
30 mM 0.0430 mL 0.2148 mL 0.4296 mL 1.0739 mL
40 mM 0.0322 mL 0.1611 mL 0.3222 mL 0.8054 mL
50 mM 0.0258 mL 0.1289 mL 0.2577 mL 0.6444 mL
60 mM 0.0215 mL 0.1074 mL 0.2148 mL 0.5370 mL
80 mM 0.0161 mL 0.0805 mL 0.1611 mL 0.4027 mL
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Cyanine 5 Tyramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyanine 5 Tyramide1431148-26-3Tyramide-Cy5DNA StainfluorescentdyeInhibitorinhibitorinhibit

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