2. Neuronal Signaling Membrane Transporter/Ion Channel Apoptosis
  3. Calcium Channel Apoptosis
  4. Cycleanine

Cycleanine is a potent vascular selective Calcium antagonist. Cycleanine has analgesic, muscle relaxant and anti-inflammatory activities. Cycleanine has potential for anti-ovarian cancer acting through the apoptosis pathway.

For research use only. We do not sell to patients.

Cycleanine Chemical Structure

Cycleanine Chemical Structure

CAS No. : 518-94-5

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Based on 1 publication(s) in Google Scholar

Cycleanine Related Antibodies

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cycleanine is a potent vascular selective Calcium antagonist. Cycleanine has analgesic, muscle relaxant and anti-inflammatory activities. Cycleanine has potential for anti-ovarian cancer acting through the apoptosis pathway[1][2].

IC50 & Target

L-type calcium channel

 

Cellular Effect
Cell Line Type Value Description References
A2780 IC50
7.6 μM
Compound: 1
Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay
Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay
[PMID: 29588214]
IGROV-1 IC50
14 μM
Compound: 1
Antiproliferative activity against human IGROV1 cells after 72 hrs by SRB assay
Antiproliferative activity against human IGROV1 cells after 72 hrs by SRB assay
[PMID: 29588214]
OVCAR-8 IC50
10 μM
Compound: 1
Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay
Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay
[PMID: 29588214]
In Vitro

Cycleanine inhibits L-type Ca-current (ICaL) of single rat ventricular cardiomyocytes in a voltage- and frequency-dependent manner[1].
Cycleanine shows modestly less potency against human OSE cells (normal) than the cancer cells[2].
Cycleanine (20 μM; 48 hours) exhibits cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC50s ranging from 7 to 14 μM[2].
Cycleanine (20 μM; 24 hours) results in significant PARP cleavage (a marker of apoptosis)[2].
Cycleanine (20 μM; 48 hours) causes a significant increase of the population of both early and late apoptotic cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cycleanine Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: Ovcar-8 cells, A2780 cells, Igrov-1 cells, Ovcar-4 cells
Concentration: 20 μM
Incubation Time: 48 hours
Result: Exhibited cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC50 values of 10 μM, 7.6 μM, 14 μM, 7.2 μM, respectively.

Western Blot Analysis[2]

Cell Line: Ovcar-8 cells
Concentration: 20 μM
Incubation Time: 24 hours
Result: Induced 1.1-fold increase in PARP-1 cleavage compared with carboplatin.

Apoptosis Analysis[2]

Cell Line: Ovcar-8 cells
Concentration: 20 μM
Incubation Time: 48 hours
Result: Caused a significant increase of the population of both early and late apoptotic cells.

Cell Cycle Analysis[2]

Cell Line: Ovcar-8 cells
Concentration: 20 μM
Incubation Time: 48 hours
Result: Increased the percentage of Ovcar-8 cells in subG1.
In Vivo

Cycleanine inhibits the KCl-induced contraction of rabbit aortic rings with an IC50 of 0.8 nM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

622.75

Formula

C38H42N2O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC1=C(OC2=CC=C(C[C@](N(CC3)C)([H])C4=C3C=C5OC)C=C2)C([C@@](N6C)([H])CC7=CC=C(OC4=C5OC)C=C7)=C(CC6)C=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (8.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6058 mL 8.0289 mL 16.0578 mL
5 mM 0.3212 mL 1.6058 mL 3.2116 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6058 mL 8.0289 mL 16.0578 mL 40.1445 mL
5 mM 0.3212 mL 1.6058 mL 3.2116 mL 8.0289 mL
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Cycleanine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cycleanine518-94-5Calcium ChannelApoptosisCa2+ channelsCa channelsVascularcalciumICaLanalgesicanti-ovarianapoptosisInhibitorinhibitorinhibit

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