Cystamine dihydrochloride

Name: Cystamine dihydrochloride
Brand: MedChemExpress
SKU: HY-W020050
Rating: 4.5 (1 reviews)

Cat. No.: HY-W020050 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Cystamine (dihydrochloride) is the disulfide form of the free thiol, cysteamine. Cystamine is an orally active transglutaminase (Tgase) inhibitor. Cystamine also has inhibition activity for caspase-3 with an IC₅₀ value of 23.6 μM. Cystamine can be used for the research of severals diseases including Huntington's disease (HD) .

For research use only. We do not sell to patients.

Cystamine dihydrochloride Chemical Structure

CAS No. : 56-17-7

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
10 g	USD 50	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Cystamine dihydrochloride:

Cystamine-d₈ dihydrochloride Get quote
Cystamine Get quote

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Caspase 3

23.6 μM (IC₅₀)

In Vitro

Cystamine (dihydrochloride) has inhibition activity for caspase-3 with an IC₅₀ value of 23.6 μM^[1].
Cystamine (0-500 μM; 0-16 h) inhibits recombinant active caspase-3 in a concentration-dependent manner^[1].
Cystamine (250 μM; 10 h) robustly increases the levels of glutathione^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Human neuroblastoma SH-SY5Y cells
Concentration:	250, 500 μM
Incubation Time:	0-16 h
Result:	Inhibited the MG132-mediated activation of caspase-3. Inhibited the H2O2-mediated activation of caspase-3. Inhibited caspase-3 activity in a tTG-independent manner.

In Vivo

Cystamine (dihydrochloride) (oral, i.p.; 112, 225 mg/kg) reduces Tgase activity and GGEL levels, lessens the behavioral and neuropathological severity, and extends survival in R6/2 transgenic HD mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	R6/2 transgenic HD mice^[2]
Dosage:	112, 225 mg/kg
Administration:	Intraperitoneal or oral, daily
Result:	Significantly extended survival, improved body weight and motor performance, delayed the neuropathological sequela and significantly altered the levels of Tgase activity and N(Sigma)-(gamma-L-glutamyl)-L-lysine (GGEL) levels.

Molecular Weight

225.20

Formula

C₄H₁₄Cl₂N₂S₂

CAS No.

56-17-7

Appearance

Solid

Color

White to off-white

SMILES

NCCSSCCN.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (444.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (444.05 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4405 mL	22.2025 mL	44.4050 mL
5 mM	0.8881 mL	4.4405 mL	8.8810 mL
10 mM	0.4440 mL	2.2202 mL	4.4405 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (452 KB)

COA (303 KB) HNMR (233 KB)

Handling Instructions (2659 KB)

References

[1]. Mathieu Lesort, et al. Cystamine inhibits caspase activity. Implications for the treatment of polyglutamine disorders. J Biol Chem. 2003 Feb 7;278(6):3825-30. [Content Brief]

[2]. Alpaslan Dedeoglu, et al. Therapeutic effects of cystamine in a murine model of Huntington's disease. J Neurosci. 2002 Oct 15;22(20):8942-50. [Content Brief]

[3]. Thomas M Jeitner, et al. Cystamine and cysteamine as inhibitors of transglutaminase activity in vivo. Biosci Rep. 2018 Sep 5;38(5):BSR20180691. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.4405 mL	22.2025 mL	44.4050 mL	111.0124 mL
	5 mM	0.8881 mL	4.4405 mL	8.8810 mL	22.2025 mL
	10 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	15 mM	0.2960 mL	1.4802 mL	2.9603 mL	7.4008 mL
	20 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	25 mM	0.1776 mL	0.8881 mL	1.7762 mL	4.4405 mL
	30 mM	0.1480 mL	0.7401 mL	1.4802 mL	3.7004 mL
	40 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL
	50 mM	0.0888 mL	0.4440 mL	0.8881 mL	2.2202 mL
	60 mM	0.0740 mL	0.3700 mL	0.7401 mL	1.8502 mL
	80 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	100 mM	0.0444 mL	0.2220 mL	0.4440 mL	1.1101 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.