DACA

Cat. No.: HY-100777 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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DACA inhibits two essential nuclear enzymes in vitro, DNA topoisomerase I and DNA topoisomerase (topo) II. DACA stabilises topo I, topo II alpha, and topo II beta cleavable complexes in human leukaemia CCRF-CEM cells.

For research use only. We do not sell to patients.

DACA Chemical Structure

CAS No. : 89459-25-6

Size	Price	Stock	Quantity
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of DACA:

DACA-d6 Get quote

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	2.3 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human DLD1 cells by Hoechst test Cytotoxicity against human DLD1 cells by Hoechst test	[PMID: 18656367]
DLD-1	IC₅₀	3.1 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human DLD1 cells by resazurin reduction test Cytotoxicity against human DLD1 cells by resazurin reduction test	[PMID: 18656367]
DU-145	IC₅₀	2 μM Compound: 8b; DACA	Antiproliferative activity against human DU-145 cells incubated for 48 hrs by Sulforhodamine B staining method Antiproliferative activity against human DU-145 cells incubated for 48 hrs by Sulforhodamine B staining method	[PMID: 33077264]
Fibroblast	IC₅₀	4.4 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human fibroblast cells by resazurin reduction test Cytotoxicity against human fibroblast cells by resazurin reduction test	[PMID: 18656367]
Fibroblast	IC₅₀	7.1 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human fibroblast cells by Hoechst test Cytotoxicity against human fibroblast cells by Hoechst test	[PMID: 18656367]
HT-29	IC₅₀	990 nM Compound: 3a	Cytotoxicity against human HT-29 cells after 144 hrs Cytotoxicity against human HT-29 cells after 144 hrs	[PMID: 18774722]
Jurkat	IC₅₀	0.3 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human Jurkat cells by Hoechst test Cytotoxicity against human Jurkat cells by Hoechst test	[PMID: 18656367]
Jurkat	IC₅₀	0.56 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human Jurkat cells by resazurin reduction test Cytotoxicity against human Jurkat cells by resazurin reduction test	[PMID: 18656367]
L1210	IC₅₀	105 nM Compound: 2	Compound concentration that reduces cell growth of L1210 leukemia cultures to 50% of controls after 70h. Compound concentration that reduces cell growth of L1210 leukemia cultures to 50% of controls after 70h.	[PMID: 3361574]
L1210	IC₅₀	98 nM Compound: 3	Inhibition of L1210 cell growth by 50% Inhibition of L1210 cell growth by 50%	[PMID: 11831901]
M4Beu cell line	IC₅₀	1.8 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human M4Beu cells by Hoechst test Cytotoxicity against human M4Beu cells by Hoechst test	[PMID: 18656367]
M4Beu cell line	IC₅₀	4.5 μM Compound: 4, DACA, NSC-601316	Cytotoxicity against human M4Beu cells by resazurin reduction test Cytotoxicity against human M4Beu cells by resazurin reduction test	[PMID: 18656367]
PC-3	IC₅₀	1.86 μM Compound: 8b; DACA	Antiproliferative activity against human PC-3 cells incubated for 48 hrs by Sulforhodamine B staining method Antiproliferative activity against human PC-3 cells incubated for 48 hrs by Sulforhodamine B staining method	[PMID: 33077264]

Molecular Weight

293.36

Formula

C₁₈H₁₉N₃O

CAS No.

89459-25-6

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=CC2=CC3=CC=CC=C3N=C12)NCCN(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (340.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4088 mL	17.0439 mL	34.0878 mL
5 mM	0.6818 mL	3.4088 mL	6.8176 mL
10 mM	0.3409 mL	1.7044 mL	3.4088 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (251 KB) HNMR (237 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Padget K, et al. An investigation into the formation of N- [2-(dimethylamino)ethyl]acridine-4-carboxamide (DACA) and 6-[2-(dimethylamino)ethylamino]- 3-hydroxy-7H-indeno[2, 1-C]quinolin-7-one dihydrochloride (TAS-103) stabilised DNA topoisomerase I and II cleavable complexes in human leukaemia cells. Biochem Pharmacol. 2000;60(6):817-821. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4088 mL	17.0439 mL	34.0878 mL	85.2195 mL
	5 mM	0.6818 mL	3.4088 mL	6.8176 mL	17.0439 mL
	10 mM	0.3409 mL	1.7044 mL	3.4088 mL	8.5220 mL
	15 mM	0.2273 mL	1.1363 mL	2.2725 mL	5.6813 mL
	20 mM	0.1704 mL	0.8522 mL	1.7044 mL	4.2610 mL
	25 mM	0.1364 mL	0.6818 mL	1.3635 mL	3.4088 mL
	30 mM	0.1136 mL	0.5681 mL	1.1363 mL	2.8407 mL
	40 mM	0.0852 mL	0.4261 mL	0.8522 mL	2.1305 mL
	50 mM	0.0682 mL	0.3409 mL	0.6818 mL	1.7044 mL
	60 mM	0.0568 mL	0.2841 mL	0.5681 mL	1.4203 mL
	80 mM	0.0426 mL	0.2130 mL	0.4261 mL	1.0652 mL
	100 mM	0.0341 mL	0.1704 mL	0.3409 mL	0.8522 mL

DACA Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DACA89459-25-6Topoisomerasehuman leukaemia CCRF-CEM cellsDNA topoisomerase I and II cleavable complexesInhibitorinhibitorinhibit

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DACA

Customer Review

Other Forms of DACA:

DACA Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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View All Topoisomerase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DACA Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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