Dibutyryl-cGMP sodium (Synonyms: Bt2cGMP sodium)

Name: Dibutyryl-cGMP sodium
Brand: MedChemExpress
SKU: HY-130354
Rating: 5.0 (2 reviews)

Cat. No.: HY-130354 Purity: 99.55%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Dibutyryl-cGMP sodium (Bt2cGMP sodium) is a cell-permeable cGMP analogue. Dibutyryl-cGMP sodium preferentially activates cGMP-dependent protein kinase (PKG). Dibutyryl-cGMP sodium inhibits the release of [³H]-arachidonic acid from γ thrombin-stimulated human platelets. Dibutyryl-cGMP sodium induces peripheral antinociception via activation of ATP-sensitive K⁺ channels.

For research use only. We do not sell to patients.

Dibutyryl-cGMP sodium Chemical Structure

CAS No. : 51116-00-8

Size	Price	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
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5 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 670	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Dibutyryl-cGMP sodium

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Description

IC₅₀ & Target

cGMP-dependent protein kinase (PKG)^[1];
ATP-sensitive K⁺ channels^[3]

In Vitro

Dibutyryl-cGMP is able to induce process elongation and branching in astrocytes resulting from a rapid, reversible and concentration-dependent redistribution of glial fibrillary acidic protein (GFAP) and actin filaments without significant change in protein levels^[1].
When cells are co-incubated with Dibutyryl-cGMP (100 μM) stress fibre formation is prevented and cells acquired a stellate morphology in cerebellar astrocytes^[1].
In cells treated with Dibutyryl-cGMP (100 μM, 2 h) the particulate fraction is nearly devoid of RhoA protein. Dibutyryl-cGMP prevents RhoA-membrane association^[1].
Using the scratchwound model, the size of the wound is significantly smaller in cells treated with Dibutyryl-cGMP after the wound indicating that dbcGMP accelerates wound closure^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dibutyryl-cGMP (50-200?μg/paw; subcutaneous injection; male Wistar rats) treatment antagonizes the hyperalgesic effect of PGE2 in a dose-dependent manner. Maximal antinociceptive effect of DbcGMP is at 1?h after administration and last for plus 2?h^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (180- 250 g) injection with Prostaglandin E2 (PGE2)^[3]
Dosage:	50 μg/paw, 75 μg/paw, 100 μg/paw and 200 μg/paw
Administration:	Subcutaneous injection
Result:	Antagonized the hyperalgesic effect of PGE2 (2 μg/paw), in a dose-dependent manner.

Molecular Weight

507.37

Formula

C₁₈H₂₃N₅NaO₉P

CAS No.

51116-00-8

Appearance

Solid

Color

White to light yellow

SMILES

CCCC(NC1=NC2=C(C(N1)=O)N=CN2[C@H]3[C@@H]([C@H]4[C@H](O3)COP(O4)(O[Na])=O)OC(CCC)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (197.09 mM; Need ultrasonic)

DMSO : 100 mg/mL (197.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9709 mL	9.8547 mL	19.7095 mL
5 mM	0.3942 mL	1.9709 mL	3.9419 mL
10 mM	0.1971 mL	0.9855 mL	1.9709 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (282 KB) SDS (252 KB)

COA (258 KB) HNMR (347 KB) CNMR (437 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Borán MS, et al. The cyclic GMP-protein kinase G pathway regulates cytoskeleton dynamics and motility in astrocytes. J Neurochem. 2007 Jul;102(1):216-30. [Content Brief]

[2]. Sane DC, et al. Cyclic GMP analogs inhibit gamma thrombin-induced arachidonic acid release in human platelets. Biochem Biophys Res Commun. 1989 Dec 15;165(2):708-14. [Content Brief]

[3]. Soares AC, et al. Dibutyryl-cyclic GMP induces peripheral antinociception via activation of ATP-sensitive K(+) channels in the rat PGE2-induced hyperalgesic paw. Br J Pharmacol. 2001 Sep;134(1):127-31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.9709 mL	9.8547 mL	19.7095 mL	49.2737 mL
	5 mM	0.3942 mL	1.9709 mL	3.9419 mL	9.8547 mL
	10 mM	0.1971 mL	0.9855 mL	1.9709 mL	4.9274 mL
	15 mM	0.1314 mL	0.6570 mL	1.3140 mL	3.2849 mL
	20 mM	0.0985 mL	0.4927 mL	0.9855 mL	2.4637 mL
	25 mM	0.0788 mL	0.3942 mL	0.7884 mL	1.9709 mL
	30 mM	0.0657 mL	0.3285 mL	0.6570 mL	1.6425 mL
	40 mM	0.0493 mL	0.2464 mL	0.4927 mL	1.2318 mL
	50 mM	0.0394 mL	0.1971 mL	0.3942 mL	0.9855 mL
	60 mM	0.0328 mL	0.1642 mL	0.3285 mL	0.8212 mL
	80 mM	0.0246 mL	0.1232 mL	0.2464 mL	0.6159 mL
	100 mM	0.0197 mL	0.0985 mL	0.1971 mL	0.4927 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Dibutyryl-cGMP sodium Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dibutyryl-cGMP51116-00-8Bt2cGMPPotassium ChannelKcsARhoA-membraneLPSastrocytesantinociceptionwoundclosurecell-permeableproteinkinaseInhibitorinhibitorinhibit

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Dibutyryl-cGMP sodium (Synonyms: Bt2cGMP sodium)

Customer Review

Dibutyryl-cGMP sodium Related Antibodies

2 Publications Citing Use of MCE Dibutyryl-cGMP sodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Dibutyryl-cGMP sodium Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Dibutyryl-cGMP sodium Related Classifications

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