2. Anti-infection
  3. Fungal
  4. Difenoconazole

Difenoconazole is a sterol demethylation inhibitor, as a fungicide. Difenoconazole binds the heme portion of the fungal cytochrome P450 51, interferes the mycelial growth and inhibits the spore germination of pathogens, suppressing fungal growth.

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Difenoconazole Chemical Structure

Difenoconazole Chemical Structure

CAS No. : 119446-68-3

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Based on 1 publication(s) in Google Scholar

Other Forms of Difenoconazole:

Difenoconazole Related Antibodies

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Description

Difenoconazole is a sterol demethylation inhibitor, as a fungicide. Difenoconazole binds the heme portion of the fungal cytochrome P450 51, interferes the mycelial growth and inhibits the spore germination of pathogens, suppressing fungal growth[1].

In Vitro

Difenoconazole inhibits the growth of Fusarium graminearum with an EC50 of 1.69-19.6 mg/L[2].
Difenoconazole inhibits growth of Alternaria solani, Fulvia fulva,Botrytis cinerea, and Rhizoctonia solani with EC50s of 0.131, 0.069, 0.297, and 0.252 mg/L, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Difenoconazole Related Antibodies
In Vivo

Difenoconazole (0.25-2 mg/L; exposure for 96 h) induces a large suite of symptoms in embryonic development, including hatching inhibition, slow heart rate, growth regression and morphological deformities[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish[1]
Dosage: 0.25, 0.5, 1, 1.5 and 2 mg/L
Administration: Exposure for 96 h
Result: Caused significant body color blackening and decrease in the heart rate of zebrafish larvae over 24 h at 0.5 mg/L.
Inhibited the growth weight of adult zebrafish measured after 14 days' exposure at 0.25 mg/L.
Molecular Weight

406.26

Formula

C19H17Cl2N3O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1OC(CN2N=CN=C2)(C3=CC=C(OC4=CC=C(Cl)C=C4)C=C3Cl)OC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (246.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4615 mL 12.3074 mL 24.6148 mL
5 mM 0.4923 mL 2.4615 mL 4.9230 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4615 mL 12.3074 mL 24.6148 mL 61.5369 mL
5 mM 0.4923 mL 2.4615 mL 4.9230 mL 12.3074 mL
10 mM 0.2461 mL 1.2307 mL 2.4615 mL 6.1537 mL
15 mM 0.1641 mL 0.8205 mL 1.6410 mL 4.1025 mL
20 mM 0.1231 mL 0.6154 mL 1.2307 mL 3.0768 mL
25 mM 0.0985 mL 0.4923 mL 0.9846 mL 2.4615 mL
30 mM 0.0820 mL 0.4102 mL 0.8205 mL 2.0512 mL
40 mM 0.0615 mL 0.3077 mL 0.6154 mL 1.5384 mL
50 mM 0.0492 mL 0.2461 mL 0.4923 mL 1.2307 mL
60 mM 0.0410 mL 0.2051 mL 0.4102 mL 1.0256 mL
80 mM 0.0308 mL 0.1538 mL 0.3077 mL 0.7692 mL
100 mM 0.0246 mL 0.1231 mL 0.2461 mL 0.6154 mL
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Difenoconazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Difenoconazole119446-68-3FungaldemethylationfungizebrafishmycelialInhibitorinhibitorinhibit

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