2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. FLAP Epoxide Hydrolase
  4. Diflapolin

Diflapolin is a highly active dual 5-lipoxygenase-activating protein (FLAP)/soluble epoxide hydrolase (sEH) inhibitor with marked anti-inflammatory efficacy and high target selectivity. Diflapolin inhibits 5-LOX product formation in intact human monocytes and neutrophils with IC50s?of? 30 and 170?nM, respectively, and suppressed the activity of isolated sEH (IC50=20?nM).

For research use only. We do not sell to patients.

Diflapolin Chemical Structure

Diflapolin Chemical Structure

CAS No. : 724453-98-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 81 In-stock
Solution
10 mM * 1 mL in DMSO USD 81 In-stock
Solid
5 mg USD 80 In-stock
10 mg USD 140 In-stock
25 mg USD 240 In-stock
50 mg USD 370 In-stock
100 mg USD 560 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Diflapolin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Diflapolin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Diflapolin is a highly active dual 5-lipoxygenase-activating protein (FLAP)/soluble epoxide hydrolase (sEH) inhibitor with marked anti-inflammatory efficacy and high target selectivity. Diflapolin inhibits 5-LOX product formation in intact human monocytes and neutrophils with IC50s?of? 30 and 170?nM, respectively, and suppressed the activity of isolated sEH (IC50=20?nM)[1].

In Vivo

Diflapolin (1-10 mg/kg; i.p.; 30?min before zymosan injection) exhibits potent anti-inflammatory properties in in-vivo experiments[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male CD-1 mice (zymosan-induced peritonitis mouse model)[1]
Dosage: 1, 3 and 10 mg/kg
Administration: I.p.; 30 min before zymosan injection
Result: Induced a significant reduction of LTC4 and LTB4 peritoneal levels, starting from the dose of 1 mg/kg and comparable to the effect of MK886.
Molecular Weight

458.36

Formula

C22H17Cl2N3O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(Cl)C(Cl)=C1)NC2=CC=C(OCC3=NC4=CC=CC=C4S3)C=C2C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (181.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1817 mL 10.9085 mL 21.8169 mL
5 mM 0.4363 mL 2.1817 mL 4.3634 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (4.54 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1817 mL 10.9085 mL 21.8169 mL 54.5423 mL
5 mM 0.4363 mL 2.1817 mL 4.3634 mL 10.9085 mL
10 mM 0.2182 mL 1.0908 mL 2.1817 mL 5.4542 mL
15 mM 0.1454 mL 0.7272 mL 1.4545 mL 3.6362 mL
20 mM 0.1091 mL 0.5454 mL 1.0908 mL 2.7271 mL
25 mM 0.0873 mL 0.4363 mL 0.8727 mL 2.1817 mL
30 mM 0.0727 mL 0.3636 mL 0.7272 mL 1.8181 mL
40 mM 0.0545 mL 0.2727 mL 0.5454 mL 1.3636 mL
50 mM 0.0436 mL 0.2182 mL 0.4363 mL 1.0908 mL
60 mM 0.0364 mL 0.1818 mL 0.3636 mL 0.9090 mL
80 mM 0.0273 mL 0.1364 mL 0.2727 mL 0.6818 mL
100 mM 0.0218 mL 0.1091 mL 0.2182 mL 0.5454 mL
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Diflapolin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diflapolin724453-98-9FLAPEpoxide Hydrolase5-lipoxygenase-activating protein5-LO activating proteinvascularpermeabilitycysteinyl-LTsneutrophilinfiltrationinflammationdiseasesInhibitorinhibitorinhibit

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