2. Others Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis Neuronal Signaling
  3. Insecticide Reactive Oxygen Species Apoptosis Cholinesterase (ChE)
  4. Dimethoate

Dimethoate is an organophosphate insecticide and acaricide. Dimethoate is an orally active acetylcholinesterase inhibitor. Dimethoate induces reactive oxygen species (ROS). Dimethoate induces DNA damage and cell apoptosis in vivo. Dimethoate affect immune system in mice.

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Dimethoate Chemical Structure

Dimethoate Chemical Structure

CAS No. : 60-51-5

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Based on 1 publication(s) in Google Scholar

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Dimethoate Related Antibodies

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Description

Dimethoate is an organophosphate insecticide and acaricide. Dimethoate is an orally active acetylcholinesterase inhibitor. Dimethoate induces reactive oxygen species (ROS). Dimethoate induces DNA damage and cell apoptosis in vivo. Dimethoate affect immune system in mice[1][2][3].

In Vitro

Dimethoate (0.1-10 μM, 24 h) inhibits cholinesterase (ChE) enzymatic activity in a concentration-dependent manner in pancreatic stellate cells (PSCs)[1].
Dimethoate (10-6-104 μM, 24 h) inhibits carboxylesterase (CbE) in a concentration-dependent manner (IC50 = 0.97 μM) in the PSCs extracts[1].
Dimethoate (0.1-10 μM, 24-96 h) decreases PSCs cell viability[1].
Dimethoate (0.1-10 μM, 1 h) induces reactive oxygen species (ROS) in the mitochondria of PSCs cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dimethoate Related Antibodies

Apoptosis Analysis[1]

Cell Line: pancreatic stellate cells (PSCs)
Concentration: 0.1, 1, 10 μM
Incubation Time: 24, 48, 72, 96 h
Result: Decreased cell viability.
In Vivo

Dimethoate (20-60 mg/kg; i.p.; 24-72 h) induces DNA damage and mitochondrial dysfunction triggering apoptosis in rat bone-marrow and peripheral blood cells[2].
Dimethoate (16 mg/kg; single p.o.) has effects on the immune system of female swiss albino mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female swiss albino mice each weighing 25-30 g[3]
Dosage: 16 mg/kg
Administration: Single oral gavage (p.o.)
Result: Showed no significant loss of body weight.
Observed a time depended reduction in spleen weigh.
Suppressed the lymphocyte proliferation responses to T-cell mitogen (Phytohemagglutinin, PHA) (HY-N7038) and B-cell mitogen (Lipopolysaccharides, LPS) (HY-D1056).
The percentage of total and active T cells were significantly decreased.
The total antibody and IgM responses to SRBC's were significantly decreased.
Caused a significant decrease in the number of plaque forming cell (PFC / 106 splenocytes) in a time dependent manner.
Animal Model: Rattus norvegicus (Wistar strain; 8-10 weeks old; 120 g; males only)[2]
Dosage: 20, 40, 60 mg/kg
Administration: Intraperitoneal injection (i.p.); 24, 48, 72 h
Result: Significant micronuclei induction and metaphase chromosome abnormalities in bone marrow cells indicated positive dose response relationship, confirming its genotoxic and cytotoxic potential.
Showed significant mitotic index decrease.
Decreased mitochondrial membrane potential (MMP), affected the cell cycle, and inflicted DNA damage, which led to cellular apoptosis of leukocytes culminating into immunotoxic effects.
Molecular Weight

229.26

Formula

C5H12NO3PS2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=P(OC)(SCC(NC)=O)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 10.42 mg/mL (45.45 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3619 mL 21.8093 mL 43.6186 mL
5 mM 0.8724 mL 4.3619 mL 8.7237 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 10 mg/mL (43.62 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.91%

SDS (480 KB)

COA (246 KB) LCMS (86 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.3619 mL 21.8093 mL 43.6186 mL 109.0465 mL
5 mM 0.8724 mL 4.3619 mL 8.7237 mL 21.8093 mL
10 mM 0.4362 mL 2.1809 mL 4.3619 mL 10.9047 mL
15 mM 0.2908 mL 1.4540 mL 2.9079 mL 7.2698 mL
20 mM 0.2181 mL 1.0905 mL 2.1809 mL 5.4523 mL
25 mM 0.1745 mL 0.8724 mL 1.7447 mL 4.3619 mL
30 mM 0.1454 mL 0.7270 mL 1.4540 mL 3.6349 mL
40 mM 0.1090 mL 0.5452 mL 1.0905 mL 2.7262 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dimethoate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dimethoate60-51-5InsecticideReactive Oxygen SpeciesApoptosisCholinesterase (ChE)insecticideplantchemotherapeuticcattlegrubcontacttoxicityinsectsInhibitorinhibitorinhibit

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