2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease Apoptosis
  3. Dopamine Receptor Endogenous Metabolite Ferroptosis
  4. Dopamine

Dopamine  (Synonyms: ASL279 free base)

Cat. No.: HY-B0451 Purity: 98.27%
COA Handling Instructions

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Dopamine is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine plays several important roles in the brain and body. Dopamine acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis.

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Dopamine Chemical Structure

Dopamine Chemical Structure

CAS No. : 51-61-6

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Based on 9 publication(s) in Google Scholar

Other Forms of Dopamine:

Dopamine Related Antibodies

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Dopamine Receptor D1 Receptor D2 Receptor D3 Receptor D4 Receptor D5 Receptor

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dopamine is a catecholamine neurotransmitter that is produced in the substantia nigra, ventral tegmental area, and hypothalamus of the brain. Dopamine plays several important roles in the brain and body[1]. Dopamine acts through D2 dopamine receptors to induce endocytosis of VEGFR2, which is critical for promoting angiogenesis[1].

IC50 & Target

Microbial Metabolite

 

D2 Dopamine Receptor

 

In Vitro

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) strongly stimulates human umbilical vein endothelial cells (HUVECs) proliferation. The stimulatory effect of VPF/VEGF is abrogated by Dopamine (1 μM; 24hours)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dopamine Related Antibodies

Cell Proliferation Assay[2]

Cell Line: HUVECs
Concentration: 1 µM
Incubation Time: 24 hours
Result: Inhibited VPF/VEGF-induced HUVEC proliferation specifically through its D2 receptor.
In Vivo

Dopamine (50 mg/kg; i.p. injection; days 1-7) strongly and selectively inhibits the vascular permeabilizing and angiogenic activities of VPF/VEGF[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Syngeneic C3Heb/FeJ mice with MOT ascites tumors[2]
Dosage: 50 mg/kg
Administration: I.p. injection; 7 days
Result: Corresponding to ~5% of the median lethal dose (LD50) in mice) beginning at 24 hours after tumor-cell injection and continuing daily for 7 days.
Molecular Weight

153.18

Formula

C8H11NO2
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

OC1=CC=C(CCN)C=C1O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (652.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5283 mL 32.6413 mL 65.2827 mL
5 mM 1.3057 mL 6.5283 mL 13.0565 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.27%

COA (244 KB) HNMR (233 KB) RP-HPLC (251 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.5283 mL 32.6413 mL 65.2827 mL 163.2067 mL
5 mM 1.3057 mL 6.5283 mL 13.0565 mL 32.6413 mL
10 mM 0.6528 mL 3.2641 mL 6.5283 mL 16.3207 mL
15 mM 0.4352 mL 2.1761 mL 4.3522 mL 10.8804 mL
20 mM 0.3264 mL 1.6321 mL 3.2641 mL 8.1603 mL
25 mM 0.2611 mL 1.3057 mL 2.6113 mL 6.5283 mL
30 mM 0.2176 mL 1.0880 mL 2.1761 mL 5.4402 mL
40 mM 0.1632 mL 0.8160 mL 1.6321 mL 4.0802 mL
50 mM 0.1306 mL 0.6528 mL 1.3057 mL 3.2641 mL
60 mM 0.1088 mL 0.5440 mL 1.0880 mL 2.7201 mL
80 mM 0.0816 mL 0.4080 mL 0.8160 mL 2.0401 mL
100 mM 0.0653 mL 0.3264 mL 0.6528 mL 1.6321 mL
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Dopamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dopamine51-61-6ASL279ASL 279ASL-279Dopamine ReceptorEndogenous MetaboliteFerroptosisHUVECsproliferationdopaminecatecholamineneurotransmitterInhibitorinhibitorinhibit

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