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  4. Doxycycline calcium

Doxycycline calcium, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline calcium shows antibacterial activity and anti-cancer cell proliferation activity.

For research use only. We do not sell to patients.

Doxycycline calcium Chemical Structure

Doxycycline calcium Chemical Structure

CAS No. : 94088-85-4

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Doxycycline calcium Related Antibodies

Top Publications Citing Use of Products

72 Publications Citing Use of MCE Doxycycline calcium

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    Doxycycline calcium purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96.  [Abstract]

    Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.

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    • Biological Activity

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    Description

    Doxycycline calcium, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline calcium shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5].

    IC50 & Target

    Tetracycline

     

    In Vitro

    Doxycycline calcium (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline calcium (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells[2].
    Doxycycline calcium (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline calcium (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Doxycycline calcium Related Antibodies

    Cell Viability Assay[2]

    Cell Line: LNT-229, G55, and U343 glioma cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 4 days
    Result: Affected growth of glioma cells only under high concentration (10 µg/mL).

    Cell Viability Assay[2]

    Cell Line: SVG cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 24 hours
    Result: Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

    Cell Proliferation Assay[4]

    Cell Line: MCF 12A, 293T cells
    Concentration: 100 ng/mL, 1 µg/mL
    Incubation Time: 96 hours
    Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

    Cell Viability Assay[5]

    Cell Line: MCF-7, MDA-MB-468 cells
    Concentration: 0-250 μM
    Incubation Time: 72 hours
    Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
    In Vivo

    Doxycycline calcium (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline calcium (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells[2].
    Doxycycline calcium (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline calcium (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].


    Modeling ON-OFF system for gene expression[6][7]
    Background
    Doxycycline is often used as an inducer in molecular biology research to induce gene expression. In cells or model animals that have constructed a Tetracycline (Tet; HY-A0107) inducible expression (Tet-ON/Tet-OFF) system, the expression of the target gene can be precisely controlled by adding or removing Doxycycline. Doxycycline can act as an inhibitor of transcriptional activation in the Tetracycline (Tc)-controlled transactivation (tTA) system, and as an inducer of transcriptional activation in the "reverse tTA' system. Doxycycline and Tetracycline both act systemically after being absorbed by the upper gastrointestinal tract. In comparison, the main advantage of Doxycycline is that it has a longer activity and can be taken twice or once a day. Although the peak concentrations of the two are similar, Doxycycline takes a shorter time to reach peak concentration and has a significantly longer half-life [6][7][8].
    Specific Modeling Methods
    Rat[8]: male • adult middle-aged (12-month-old) • Sprague-Dawley rats
    Administartion (for GDNF regulation): 3g/kg (Doxycycline; HY-N05655) (dietary with regular food) • po once daily for 6 days • monitored every day
    Note
    1. In this study[7], a recombinant adeno-associated virus (rAAV)-based bicistronic tetracycline (tet)-OFF construct was used for dynamic control of GDNF (target gene) expression during long-term expression.
    2. 3g/kg dietary DOX produced DOX serum levels equivalent to 1mg/ml DOX in drinking water.
    Modeling Indicators
    (1) The expression level of the target gene decreases; (2) The positively correlated phenotype corresponding to the target gene is alleviated.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3]
    Dosage: 200 or 800 mg/kg
    Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months
    Result: Reduced MMP-9 activity in a dose-dependent manner.
    Clinical Trial
    Molecular Weight

    524.59

    Formula

    C22H24Ca2N2O8
    CAS No.
    SMILES

    O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.[Ca].[Ca]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Doxycycline calcium Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Doxycycline94088-85-4MMPBacterialAntibioticParasiteMatrix metalloproteinasesantibacterial activityanti-proliferative activityantibioticLNT-229G55U343SVGMCF 12A293TInhibitorinhibitorinhibit

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