EBOV/MARV-IN-1

Name: EBOV/MARV-IN-1
Brand: MedChemExpress
SKU: HY-137498
Rating: 4.5 (1 reviews)

Cat. No.: HY-137498 Purity: 99.14%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

EBOV/MARV-IN-1 is a potent inhibitor of Ebola virus (EBOV) and Marburg virus (MARV), with broad-spectrum activity (EC₅₀=0.31, and 0.82 µM, respectively) and low cytotoxicity (SI>100) in HeLa cells.

For research use only. We do not sell to patients.

EBOV/MARV-IN-1 Chemical Structure

CAS No. : 2479465-67-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 165	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 165	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Filovirus Isoform Specific Products:

View All Isoforms

EBOV MARV ZEBOV

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EBOV

MARV

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.012 μM Compound: 35	Inhibition of pseudotyped HIV/EBOV GP infected in human A549 cells assessed as reduction in virus entry measured after 48 hrs by luciferase reporter gene assay Inhibition of pseudotyped HIV/EBOV GP infected in human A549 cells assessed as reduction in virus entry measured after 48 hrs by luciferase reporter gene assay	[PMID: 32490678]
A549	EC₅₀	0.18 μM Compound: 35	Inhibition of pseudotyped HIV/MARV GP infected in human A549 cells assessed as reduction in virus entry measured after 48 hrs by luciferase reporter gene assay Inhibition of pseudotyped HIV/MARV GP infected in human A549 cells assessed as reduction in virus entry measured after 48 hrs by luciferase reporter gene assay	[PMID: 32490678]
HeLa	EC₅₀	0.31 μM Compound: 35	Antiviral activity against Ebola virus 1976 Mayinga infected in human HeLa cells pretreated for 1 hr followed by virus infection measured after 24 hrs by Hoechst staining-based immunofluorescence assay Antiviral activity against Ebola virus 1976 Mayinga infected in human HeLa cells pretreated for 1 hr followed by virus infection measured after 24 hrs by Hoechst staining-based immunofluorescence assay	[PMID: 32490678]
HeLa	EC₅₀	0.82 μM Compound: 35	Antiviral activity against Marburg virus Lake Victoria, 2005 Angola infected in human HeLa cells pretreated for 1 hr followed by virus infection measured after 24 hrs by Hoechst staining-based immunofluorescence assay Antiviral activity against Marburg virus Lake Victoria, 2005 Angola infected in human HeLa cells pretreated for 1 hr followed by virus infection measured after 24 hrs by Hoechst staining-based immunofluorescence assay	[PMID: 32490678]

In Vitro

EBOV/MARV-IN-1 (12.5 µM; 48 h) inhibits HIV/EBOV-GP (EC₅₀=12 nM) and HIV/MARV-GP (EC₅₀=180 nM) pseudotype virus in A549 cells containing a luciferase reporter gene, with low cytotoxicity (SI =2088)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

461.52

Formula

C₂₅H₃₀F₃N₃O₂

CAS No.

2479465-67-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(N2CCC(C)CC2)C(C(F)(F)F)=C1)C3=CC=C(CN4CCOCC4)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (216.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1668 mL	10.8338 mL	21.6675 mL
5 mM	0.4334 mL	2.1668 mL	4.3335 mL
10 mM	0.2167 mL	1.0834 mL	2.1668 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.14%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (245 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Gaisina IN, et, al. Discovery and Structural Optimization of 4-(Aminomethyl)benzamides as Potent Entry Inhibitors of Ebola and Marburg Virus Infections. J Med Chem. 2020 Jul 9;63(13):7211-7225. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1668 mL	10.8338 mL	21.6675 mL	54.1688 mL
	5 mM	0.4334 mL	2.1668 mL	4.3335 mL	10.8338 mL
	10 mM	0.2167 mL	1.0834 mL	2.1668 mL	5.4169 mL
	15 mM	0.1445 mL	0.7223 mL	1.4445 mL	3.6113 mL
	20 mM	0.1083 mL	0.5417 mL	1.0834 mL	2.7084 mL
	25 mM	0.0867 mL	0.4334 mL	0.8667 mL	2.1668 mL
	30 mM	0.0722 mL	0.3611 mL	0.7223 mL	1.8056 mL
	40 mM	0.0542 mL	0.2708 mL	0.5417 mL	1.3542 mL
	50 mM	0.0433 mL	0.2167 mL	0.4334 mL	1.0834 mL
	60 mM	0.0361 mL	0.1806 mL	0.3611 mL	0.9028 mL
	80 mM	0.0271 mL	0.1354 mL	0.2708 mL	0.6771 mL
	100 mM	0.0217 mL	0.1083 mL	0.2167 mL	0.5417 mL

EBOV/MARV-IN-1 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EBOV/MARV-IN-12479465-67-1FilovirusEbolavirusEBOVMarburgMARVInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

EBOV/MARV-IN-1

Customer Review

EBOV/MARV-IN-1 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Filovirus Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

EBOV/MARV-IN-1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

EBOV/MARV-IN-1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report