2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. E1/E2/E3 Enzyme NF-κB Reactive Oxygen Species SOD
  4. EN106

EN106 is a potent inhibitor of FEMIB. EN106 is a cysteine-reactive covalent ligand. EN106 disrupts recognition of the key reductive stress substrate of FEM1B, FNIP1. EN106 reduces oxidative stress and rescues high glucose-induced impaired angiogenesis in HUVECs.

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EN106 Chemical Structure

EN106 Chemical Structure

CAS No. : 757192-67-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

EN106 Related Antibodies

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Description

EN106 is a potent inhibitor of FEMIB. EN106 is a cysteine-reactive covalent ligand. EN106 disrupts recognition of the key reductive stress substrate of FEM1B, FNIP1. EN106 reduces oxidative stress and rescues high glucose-induced impaired angiogenesis in HUVECs[1][2].

In Vitro

EN106 (1 μM, 24 h) reduces the MDA content in HUVECs, inhibits the ROS generation and the activation of NF-κB signaling pathway, increases SOD activity, exhibiting antioxidant effects[2].
EN106 (1 μM, 24 h) increases the expression of cell cycle-related proteins (Cyclin D1, Cyclin D3) and VEGFA, promotes the cell proliferation, migration and angiogenesis in HUVEC, rescuing high glucose-impaired angiogenesis of HUVECs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EN106 Related Antibodies

Western Blot Analysis[2]

Cell Line: HUVEC
Concentration: 1 μM
Incubation Time: 24 h
Result: Increased the expression of cell cycle-related proteins (Cyclin D1, Cyclin D3) and VEGFA.

Cell Migration Assay [2]

Cell Line: HUVEC
Concentration: 1 μM
Incubation Time: 24 h
Result: Promoted cell migration.
In Vivo

EN106 (HA-PBA-FA/EN106 hydrogel, topical application, 6 doses in 2 weeks) promotes the wound healing, reduces the inflammatory response, and promotes angiogenesis in mouse Streptozotocin (HY-13753)-induced type 2 diabetes models[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mouse Streptozotocin (HY-13753)-induced type 2 diabetes models[2]
Dosage:
Administration: HA-PBA-FA/EN106 hydrogel, topical application, administration on days 0, 3, 5, 7, 10, and 14 after wound establishment.
Result: Accelerated the wound healing, promoted collagen deposition, upregulated the expression of FNIP1 and VEGFA proteins, increased the number of CD31(+) cells, and decreased the ROS level.
Molecular Weight

280.71

Formula

C13H13ClN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCC#N)C1=CC=C(OCCO2)C2=C1)CCl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (890.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5624 mL 17.8119 mL 35.6238 mL
5 mM 0.7125 mL 3.5624 mL 7.1248 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5624 mL 17.8119 mL 35.6238 mL 89.0596 mL
5 mM 0.7125 mL 3.5624 mL 7.1248 mL 17.8119 mL
10 mM 0.3562 mL 1.7812 mL 3.5624 mL 8.9060 mL
15 mM 0.2375 mL 1.1875 mL 2.3749 mL 5.9373 mL
20 mM 0.1781 mL 0.8906 mL 1.7812 mL 4.4530 mL
25 mM 0.1425 mL 0.7125 mL 1.4250 mL 3.5624 mL
30 mM 0.1187 mL 0.5937 mL 1.1875 mL 2.9687 mL
40 mM 0.0891 mL 0.4453 mL 0.8906 mL 2.2265 mL
50 mM 0.0712 mL 0.3562 mL 0.7125 mL 1.7812 mL
60 mM 0.0594 mL 0.2969 mL 0.5937 mL 1.4843 mL
80 mM 0.0445 mL 0.2226 mL 0.4453 mL 1.1132 mL
100 mM 0.0356 mL 0.1781 mL 0.3562 mL 0.8906 mL
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EN106 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EN106757192-67-9EN 106EN-106E1/E2/E3 EnzymeNF-κBReactive Oxygen SpeciesSODE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseNuclear factor-κBNuclear factor-kappaBSuperoxide DismutaseFEMIBcysteine-reactivecovalent ligandrecognitionreductive stressFNIP1Inhibitorinhibitorinhibit

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