2. Metabolic Enzyme/Protease
  3. E1/E2/E3 Enzyme
  4. EN219

EN219 is a moderately selective synthetic covalent ligand against an N-terminal cysteine (C8) of RNF114 with an IC50 of 470 nM. EN219 inhibits RNF114-mediated autoubiquitination and p21 ubiquitination.

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EN219 Chemical Structure

EN219 Chemical Structure

CAS No. : 380351-29-1

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Based on 1 publication(s) in Google Scholar

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EN219 Related Antibodies

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Description

EN219 is a moderately selective synthetic covalent ligand against an N-terminal cysteine (C8) of RNF114 with an IC50 of 470 nM. EN219 inhibits RNF114-mediated autoubiquitination and p21 ubiquitination[1].

IC50 & Target[1]

RNF114

470 nM (IC50)

In Vitro

EN219 (1 μM; 90 min) interacts with RNF114 C8, TUBB1 C201, HSPD1 C442, and HIST1H3A C97 demonstrated by isotopic tandem orthogonal proteolysisABPP (isoTOP-ABPP) analysis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EN219 Related Antibodies
Molecular Weight

456.56

Formula

C17H13Br2ClN2O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

ClCC(N1N=C(C2=CC=C(Br)C=C2)CC1C3=CC=C(Br)C=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 65 mg/mL (142.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1903 mL 10.9515 mL 21.9029 mL
5 mM 0.4381 mL 2.1903 mL 4.3806 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1903 mL 10.9515 mL 21.9029 mL 54.7573 mL
5 mM 0.4381 mL 2.1903 mL 4.3806 mL 10.9515 mL
10 mM 0.2190 mL 1.0951 mL 2.1903 mL 5.4757 mL
15 mM 0.1460 mL 0.7301 mL 1.4602 mL 3.6505 mL
20 mM 0.1095 mL 0.5476 mL 1.0951 mL 2.7379 mL
25 mM 0.0876 mL 0.4381 mL 0.8761 mL 2.1903 mL
30 mM 0.0730 mL 0.3650 mL 0.7301 mL 1.8252 mL
40 mM 0.0548 mL 0.2738 mL 0.5476 mL 1.3689 mL
50 mM 0.0438 mL 0.2190 mL 0.4381 mL 1.0951 mL
60 mM 0.0365 mL 0.1825 mL 0.3650 mL 0.9126 mL
80 mM 0.0274 mL 0.1369 mL 0.2738 mL 0.6845 mL
100 mM 0.0219 mL 0.1095 mL 0.2190 mL 0.5476 mL
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EN219 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EN219380351-29-1EN 219EN-219E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseautoubiquitinationp21RNF114 C8TUBB1 C201HSPD1 C442HIST1H3A C97Inhibitorinhibitorinhibit

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