2. Protein Tyrosine Kinase/RTK JAK/STAT Signaling Others
  3. EGFR Others
  4. EphB1-IN-1

EphB1-IN-1 (Compound 1) is a potent EphB1 inhibitor with IC50s of 3.0, 15 and 220 nM against EphB1G703C, EphB1T697G and EphB1WT, respectively.

For research use only. We do not sell to patients.

EphB1-IN-1 Chemical Structure

EphB1-IN-1 Chemical Structure

CAS No. : 1980036-18-7

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5 mg USD 360 In-stock
10 mg USD 580 In-stock
25 mg USD 1150 In-stock
50 mg USD 1850 In-stock
100 mg USD 3000 In-stock
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Based on 1 publication(s) in Google Scholar

EphB1-IN-1 Related Antibodies

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

EphB1-IN-1 (Compound 1) is a potent EphB1 inhibitor with IC50s of 3.0, 15 and 220 nM against EphB1G703C, EphB1T697G and EphB1WT, respectively[1].

IC50 & Target

IC50: 3.0 nM (EphB1G703C), 15 nM (EphB1T697G), 220 nM (EphB1WT)[1]
In Vitro

EphB1-IN-1 (Compound 1) and 3MB-PP1 (HY-102069) cause orthogonal inhibition of two Eph kinases in the same cell[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EphB1-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK293 cells
Concentration: 300 nM
Incubation Time:
Result: Inhibited EphB1G703C. Abolished the autophosphorylation of EphB3WT with little effect on that of EphB1T697G. 100 nM of 3MB-PP1 abolished the autophosphorylation of EphB1T697G but had little effect on that of EphB3WT.
Molecular Weight

363.20

Formula

C16H12Cl2N4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC2=NC=NC(NC3=CC(O)=CC=C3Cl)=C2C=C1)CCl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (413.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7533 mL 13.7665 mL 27.5330 mL
5 mM 0.5507 mL 2.7533 mL 5.5066 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.75 mg/mL (10.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7533 mL 13.7665 mL 27.5330 mL 68.8326 mL
5 mM 0.5507 mL 2.7533 mL 5.5066 mL 13.7665 mL
10 mM 0.2753 mL 1.3767 mL 2.7533 mL 6.8833 mL
15 mM 0.1836 mL 0.9178 mL 1.8355 mL 4.5888 mL
20 mM 0.1377 mL 0.6883 mL 1.3767 mL 3.4416 mL
25 mM 0.1101 mL 0.5507 mL 1.1013 mL 2.7533 mL
30 mM 0.0918 mL 0.4589 mL 0.9178 mL 2.2944 mL
40 mM 0.0688 mL 0.3442 mL 0.6883 mL 1.7208 mL
50 mM 0.0551 mL 0.2753 mL 0.5507 mL 1.3767 mL
60 mM 0.0459 mL 0.2294 mL 0.4589 mL 1.1472 mL
80 mM 0.0344 mL 0.1721 mL 0.3442 mL 0.8604 mL
100 mM 0.0275 mL 0.1377 mL 0.2753 mL 0.6883 mL
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EphB1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EphB1-IN-11980036-18-7EGFROthersEpidermal growth factor receptorErbB-1HER13MB-PP1orthogonal inhibitionHEK293Inhibitorinhibitorinhibit

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