Ethidium bromide (Synonyms: EtBr; Homidium bromide)

Name: Ethidium bromide
Brand: MedChemExpress
SKU: HY-D0021
Rating: 5.0 (10 reviews)

Cat. No.: HY-D0021 Purity: 99.85%: FAQs
Data Sheet SDS COA Handling Instructions

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Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

For research use only. We do not sell to patients.

Ethidium bromide Chemical Structure

CAS No. : 1239-45-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE Ethidium bromide

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Biological Activity
Purity & Documentation
References
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Description

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	IC₅₀	2.33 μM Compound: Ethidium bromide	Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MDA-MB-231 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25965778]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA	[PMID: 25554922]
MGC-803	IC₅₀	2.1 μM Compound: Ethidium bromide	Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA Inhibition of telomerase in human MGC803 cell extracts after 24 hrs by TRAP-PCR-ELISA	[PMID: 24119869]
MGC-803	IC₅₀	2.18 μM Compound: 2	Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method Inhibition of telomerase in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA method	[PMID: 26883149]
MGC-803	IC₅₀	2.39 μM Compound: Ethidium bromide	Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase-mediated supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 23265905]
MGC-803	IC₅₀	2.47 μM Compound: Ethidium bromide	Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase supercoiling activity in human MGC803 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 22405648]
NIH3T3	IC₅₀	134.87 μM Compound: Ethidium bromide	Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay	[PMID: 24286761]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20947362]
SGC-7901	IC₅₀	2.5 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 20800480]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase in human SGC7901 cells by TRAP assay Inhibition of telomerase in human SGC7901 cells by TRAP assay	[PMID: 23312949]
SGC-7901	IC₅₀	2.6 μM Compound: Ethidium bromide	Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP Inhibition of telomerase activity expressed in human SGC7901 cells by SYBER green dye based TRAP	[PMID: 21486698]
SW 1116	IC₅₀	2.71 μM Compound: Ethidium bromide	Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay	[PMID: 24286761]

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).
4. Make the solution cool down before solidification.
5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).
6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

394.31

Formula

C₂₁H₂₀BrN₃

CAS No.

1239-45-8

Appearance

Solid

Color

Brown to reddish brown

SMILES

CC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (63.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5361 mL	12.6804 mL	25.3608 mL
5 mM	0.5072 mL	2.5361 mL	5.0722 mL
10 mM	0.2536 mL	1.2680 mL	2.5361 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 9.09 mg/mL (23.05 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (277 KB) SDS (644 KB)

COA (252 KB) HNMR (370 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [Content Brief]

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5361 mL	12.6804 mL	25.3608 mL	63.4019 mL
	5 mM	0.5072 mL	2.5361 mL	5.0722 mL	12.6804 mL
	10 mM	0.2536 mL	1.2680 mL	2.5361 mL	6.3402 mL
	15 mM	0.1691 mL	0.8454 mL	1.6907 mL	4.2268 mL
	20 mM	0.1268 mL	0.6340 mL	1.2680 mL	3.1701 mL
	25 mM	0.1014 mL	0.5072 mL	1.0144 mL	2.5361 mL
	30 mM	0.0845 mL	0.4227 mL	0.8454 mL	2.1134 mL
	40 mM	0.0634 mL	0.3170 mL	0.6340 mL	1.5850 mL
	50 mM	0.0507 mL	0.2536 mL	0.5072 mL	1.2680 mL
	60 mM	0.0423 mL	0.2113 mL	0.4227 mL	1.0567 mL

Ethidium bromide Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethidium1239-45-8EtBrHomidiumDNA StainInhibitorinhibitorinhibit

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Ethidium bromide (Synonyms: EtBr; Homidium bromide)

Customer Review

Ethidium bromide Related Antibodies

10 Publications Citing Use of MCE Ethidium bromide

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Ethidium bromide Related Antibodies

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Complete Stock Solution Preparation Table

Ethidium bromide Related Classifications

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