Factor D inhibitor 6

Name: Factor D inhibitor 6
Brand: MedChemExpress
SKU: HY-122700
Rating: 4.5 (1 reviews)

Cat. No.: HY-122700 Purity: 99.45%: FAQs
Data Sheet SDS COA Handling Instructions

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Factor D inhibitor 6 is a potent, highly selective and orally active factor D (FD) inhibitor with an IC₅₀ of 30 nM and a K_d of 6 nM. Factor D inhibitor 6 is inactive against factor B, lassical and lectin complement-pathway activation, and a broad assay panel of receptors, ion channels, kinases and proteases.

For research use only. We do not sell to patients.

Factor D inhibitor 6 Chemical Structure

CAS No. : 1386455-51-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 649	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 649	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 236	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 590	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1900	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3050	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4900	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: 30 nM (Factor D)^[1]
Kd: 6 nM (Factor D)^[1]

In Vitro

Factor D inhibitor 6 (compound 6) effectively blocks both alternative pathway (AP)-mediated hemolysis in 10% human serum (IC₅₀ = 6 nM) and AP-induced membrane-attack complex (MAC) formation in lepirudinanticoagulated 50% human whole blood (IC₅₀ = 0.14 μM)^[1].
Factor D inhibitor 6 (compound 6) shows modest inhibition of murine FD (IC₅₀ = 0.86 μM)^[1].
Factor D inhibitor 6 (compound 6) inhibits both hemolysis and component 3 (C3) deposition on the surface of red blood cells (RBCs) with an IC₅₀ value of 70 nM, consistent with inhibition of the AP amplification loop^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Factor D inhibitor 6 (Compound 6; 1-10 mg/kg; Oral gavage; once; C57Bl/6 mice) treatment dosed-ependently inhibits complement activation, with full inhibition at 10 mg/kg. Factor D inhibitor 6 shows sustained inhibition of LPS-induced AP activation for at least 8 h post-dose with an EC₅₀ of 0.034 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6 mice induced by lipopolysaccharide (LPS)^[1]
Dosage:	1 mg/kg, 3 mg/kg, 10 mg/kg
Administration:	Oral gavage; once
Result:	Dosed-ependently inhibited complement activation, with full inhibition at 10 mg/kg.

Molecular Weight

484.91

Formula

C₂₃H₂₂ClFN₆O₃

CAS No.

1386455-51-1

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1N(C(CN2N=C(C(N)=O)C3=C2C=NC=C3)=O)[C@]4([H])C[C@]4([H])C1)N[C@@H](C5=CC=CC(Cl)=C5F)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (515.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0622 mL	10.3112 mL	20.6224 mL
5 mM	0.4124 mL	2.0622 mL	4.1245 mL
10 mM	0.2062 mL	1.0311 mL	2.0622 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (282 KB) SDS (251 KB)

COA (262 KB) HNMR (299 KB) RP-HPLC (245 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Jürgen Maibaum, et al. Small-molecule Factor D Inhibitors Targeting the Alternative Complement Pathway. Nat Chem Biol. 2016 Dec;12(12):1105-1110. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0622 mL	10.3112 mL	20.6224 mL	51.5560 mL
	5 mM	0.4124 mL	2.0622 mL	4.1245 mL	10.3112 mL
	10 mM	0.2062 mL	1.0311 mL	2.0622 mL	5.1556 mL
	15 mM	0.1375 mL	0.6874 mL	1.3748 mL	3.4371 mL
	20 mM	0.1031 mL	0.5156 mL	1.0311 mL	2.5778 mL
	25 mM	0.0825 mL	0.4124 mL	0.8249 mL	2.0622 mL
	30 mM	0.0687 mL	0.3437 mL	0.6874 mL	1.7185 mL
	40 mM	0.0516 mL	0.2578 mL	0.5156 mL	1.2889 mL
	50 mM	0.0412 mL	0.2062 mL	0.4124 mL	1.0311 mL
	60 mM	0.0344 mL	0.1719 mL	0.3437 mL	0.8593 mL
	80 mM	0.0258 mL	0.1289 mL	0.2578 mL	0.6444 mL
	100 mM	0.0206 mL	0.1031 mL	0.2062 mL	0.5156 mL