FAPI-4

Name: FAPI-4
Brand: MedChemExpress
SKU: HY-128643
Rating: 5.0 (21 reviews)

Cat. No.: HY-128643 Purity: 98.34%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

FAPI-4 is a potent fibroblast activation protein (FAP) inhibitor for the targeting FAP. FAPI-4 can be used in cancer research. ⁶⁸Ga-FAPI-4 PET/CT is a promising new diagnostic method for imaging various kinds of cancer, with good tumor-to-background contrast ratios.

For research use only. We do not sell to patients.

FAPI-4 Chemical Structure

CAS No. : 2374782-02-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 1200	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 1200	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 312	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3950	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 21 publication(s) in Google Scholar

21 Publications Citing Use of MCE FAPI-4

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Biological Activity
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Description

IC₅₀ & Target

Fibroblast activation protein (FAP)^[1]

In Vivo

FAPI-4 (Intravenous injection; 30 nmol per mouse; once) shows excellent tumor uptake in BALB/c nu/nu mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old BALB/c nu/nu mice inoculated with HT-1080-FAP cells^[2]
Dosage:	30 nmol per mouse
Administration:	Intravenous injection; 30 nmol per mouse; once
Result:	Revealed the high overall tumor uptake (9.44%ID/g 4 h after injection).

Molecular Weight

872.91

Formula

C₄₀H₅₄F₂N₁₀O₁₀

CAS No.

2374782-02-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC(N1CC(F)(F)C[C@]1(C#N)[H])=O)C2=CC=NC3=CC=C(OCCCN4CCN(C(CN5CCN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC5)=O)CC4)C=C32

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (114.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1456 mL	5.7280 mL	11.4559 mL
5 mM	0.2291 mL	1.1456 mL	2.2912 mL
10 mM	0.1146 mL	0.5728 mL	1.1456 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline
Solubility: 4 mg/mL (4.58 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.42%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (248 KB) HNMR (265 KB) LCMS (138 KB)

Handling Instructions (2659 KB)

References

[1]. Giesel FL, et al. 68Ga-FAPI PET/CT: Biodistribution and Preliminary Dosimetry Estimate of 2 DOTA-Containing FAP-Targeting Agents in Patients with Various Cancers. J Nucl Med. 2019 Mar;60(3):386-392. [Content Brief]

[2]. Anastasia Loktev, et al. Development of Fibroblast Activation Protein-Targeted Radiotracers with Improved Tumor Retention. J Nucl Med. 2019 Oct;60(10):1421-1429. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1456 mL	5.7280 mL	11.4559 mL	28.6398 mL
	5 mM	0.2291 mL	1.1456 mL	2.2912 mL	5.7280 mL
	10 mM	0.1146 mL	0.5728 mL	1.1456 mL	2.8640 mL
	15 mM	0.0764 mL	0.3819 mL	0.7637 mL	1.9093 mL
	20 mM	0.0573 mL	0.2864 mL	0.5728 mL	1.4320 mL
	25 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1456 mL
	30 mM	0.0382 mL	0.1909 mL	0.3819 mL	0.9547 mL
	40 mM	0.0286 mL	0.1432 mL	0.2864 mL	0.7160 mL
	50 mM	0.0229 mL	0.1146 mL	0.2291 mL	0.5728 mL
	60 mM	0.0191 mL	0.0955 mL	0.1909 mL	0.4773 mL
	80 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.3580 mL
	100 mM	0.0115 mL	0.0573 mL	0.1146 mL	0.2864 mL

FAPI-4 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FAPI-42374782-02-0FAPI4FAPI 4FAPFibroblast activation proteinradiotracer tissue kineticscancer-associated fibroblastsPET/CTbiodistributiondosimetryfibroblast activation proteinInhibitorinhibitorinhibit

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FAPI-4

Customer Review

FAPI-4 Related Antibodies

21 Publications Citing Use of MCE FAPI-4

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

FAPI-4 Related Classifications

Keywords:

Your Recently Viewed Products:

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