2. Stem Cell/Wnt
  3. Wnt
  4. Foxy-5 TFA

Foxy-5 TFA, a WNT5A agonist, is a mimicking peptide of WNT5A which is a non-canonical member of the Wnt family. Foxy-5 TFA triggers cytosolic free calcium signaling without affecting β-catenin activation and it impairs the migration and invasion of epithelial cancer cells. Foxy-5 TFA effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Foxy-5 TFA Chemical Structure

Foxy-5 TFA Chemical Structure

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1 mg USD 120 In-stock
5 mg USD 350 In-stock
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Based on 4 publication(s) in Google Scholar

Other Forms of Foxy-5 TFA:

Foxy-5 TFA Related Antibodies

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Description

Foxy-5 TFA, a WNT5A agonist, is a mimicking peptide of WNT5A which is a non-canonical member of the Wnt family. Foxy-5 TFA triggers cytosolic free calcium signaling without affecting β-catenin activation and it impairs the migration and invasion of epithelial cancer cells. Foxy-5 TFA effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model[1][2][3].

IC50 & Target

WNT5A[2]
In Vitro

Foxy-5 TFA (100 μM, 24 h) inhibits the invasion of tumor cells in DU145 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Foxy-5 TFA Related Antibodies

Cell Invasion Assay[3]

Cell Line: DU145, PC3
Concentration: 100 μM
Incubation Time: 24 h
Result: Reduced DU145 cell invasion by 40%.
In Vivo

Foxy-5 TFA (2 mg/kg intraperitoneally injected 9 times a day for 7-23 days) reduces the number of colon cancer stem cells in a mouse model of human colon cancer xenotransplantation[2].
Foxy-5 TFA (2 mg/kg, intraperitoneal injection, once a day, for 3-9 weeks) significantly reduces the early metastasis and spread of WNT5A low DU145 prostate cancer cells in a mouse model of prostate cancer xenotransplantation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A Xenograft Mouse Model of Human Colonic Cancer[2]
Dosage: 2 mg/kg
Administration: i.p. 9 times a day for 7-23 days
Result: Reduced the expression of ALDH, DCLK1 and COX-2.
Increased the expression of 15-PGDH.
Reduced the expression of the downstream β-catenin target ASCL2.
Animal Model: A Xenograft Mouse Model of Prostate Cancer [3]
Dosage: 2 mg/kg
Administration: i.p. once a day, for 3-9 weeks
Result: Reduced metastatic spread and the number of tumor cells in lymph nodes.
Clinical Trial
Molecular Weight

808.80

Formula

C28H43F3N6O14S2
Appearance

Solid

Color

White to off-white

Sequence

{For}-Met-Asp-Gly-Cys-Glu-Leu
Sequence Shortening

{For}-MDGCEL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 2 mg/mL (2.47 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2364 mL 6.1820 mL 12.3640 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 7.14 mg/mL (8.83 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  Saline

    Solubility: 5 mg/mL (6.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2364 mL 6.1820 mL 12.3640 mL 30.9100 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Foxy-5 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Foxy-5Foxy5Foxy 5Wntmimickingpeptideanti-metastaticmigrationinvasionprostatecancerInhibitorinhibitorinhibit

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