2. Stem Cell/Wnt
  3. Wnt
  4. Foxy-5 TFA

Foxy-5 TFA, a WNT5A agonist, is a mimicking peptide of WNT5A which is a non-canonical member of the Wnt family. Foxy-5 TFA triggers cytosolic free calcium signaling without affecting β-catenin activation and it impairs the migration and invasion of epithelial cancer cells. Foxy-5 TFA effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model.

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Foxy-5 TFA Chemical Structure

Foxy-5 TFA Chemical Structure

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Based on 4 publication(s) in Google Scholar

Other Forms of Foxy-5 TFA:

Foxy-5 TFA Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Foxy-5 TFA, a WNT5A agonist, is a mimicking peptide of WNT5A which is a non-canonical member of the Wnt family. Foxy-5 TFA triggers cytosolic free calcium signaling without affecting β-catenin activation and it impairs the migration and invasion of epithelial cancer cells. Foxy-5 TFA effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model[1][2][3].

IC50 & Target

WNT5A[2]
In Vitro

Foxy-5 TFA (100 μM, 24 h) inhibits the invasion of tumor cells in DU145 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Foxy-5 TFA Related Antibodies

Cell Invasion Assay[3]

Cell Line: DU145, PC3
Concentration: 100 μM
Incubation Time: 24 h
Result: Reduced DU145 cell invasion by 40%.
In Vivo

Foxy-5 TFA (2 mg/kg intraperitoneally injected 9 times a day for 7-23 days) reduces the number of colon cancer stem cells in a mouse model of human colon cancer xenotransplantation[2].
Foxy-5 TFA (2 mg/kg, intraperitoneal injection, once a day, for 3-9 weeks) significantly reduces the early metastasis and spread of WNT5A low DU145 prostate cancer cells in a mouse model of prostate cancer xenotransplantation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A Xenograft Mouse Model of Human Colonic Cancer[2]
Dosage: 2 mg/kg
Administration: i.p. 9 times a day for 7-23 days
Result: Reduced the expression of ALDH, DCLK1 and COX-2.
Increased the expression of 15-PGDH.
Reduced the expression of the downstream β-catenin target ASCL2.
Animal Model: A Xenograft Mouse Model of Prostate Cancer [3]
Dosage: 2 mg/kg
Administration: i.p. once a day, for 3-9 weeks
Result: Reduced metastatic spread and the number of tumor cells in lymph nodes.
Clinical Trial
Molecular Weight

808.80

Formula

C28H43F3N6O14S2
Appearance

Solid

Color

White to off-white

Sequence

{For}-Met-Asp-Gly-Cys-Glu-Leu
Sequence Shortening

{For}-MDGCEL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (123.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 2 mg/mL (2.47 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2364 mL 6.1820 mL 12.3640 mL
5 mM 0.2473 mL 1.2364 mL 2.4728 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 7.14 mg/mL (8.83 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  Saline

    Solubility: 5 mg/mL (6.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2364 mL 6.1820 mL 12.3640 mL 30.9100 mL
DMSO 5 mM 0.2473 mL 1.2364 mL 2.4728 mL 6.1820 mL
10 mM 0.1236 mL 0.6182 mL 1.2364 mL 3.0910 mL
15 mM 0.0824 mL 0.4121 mL 0.8243 mL 2.0607 mL
20 mM 0.0618 mL 0.3091 mL 0.6182 mL 1.5455 mL
25 mM 0.0495 mL 0.2473 mL 0.4946 mL 1.2364 mL
30 mM 0.0412 mL 0.2061 mL 0.4121 mL 1.0303 mL
40 mM 0.0309 mL 0.1545 mL 0.3091 mL 0.7727 mL
50 mM 0.0247 mL 0.1236 mL 0.2473 mL 0.6182 mL
60 mM 0.0206 mL 0.1030 mL 0.2061 mL 0.5152 mL
80 mM 0.0155 mL 0.0773 mL 0.1545 mL 0.3864 mL
100 mM 0.0124 mL 0.0618 mL 0.1236 mL 0.3091 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Foxy-5 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Foxy-5Foxy5Foxy 5Wntmimickingpeptideanti-metastaticmigrationinvasionprostatecancerInhibitorinhibitorinhibit

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