G6PDi-1

Name: G6PDi-1
Brand: MedChemExpress
SKU: HY-W107464
Rating: 5.0 (2 reviews)

Cat. No.: HY-W107464 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

G6PDi-1 is a reversible and non-competitive glucose-6-phosphate dehydrogenase (G6PD) inhibitor with an IC₅₀ of 0.07 μM for human G6PD. G6PDi-1 depletes NADPH most strongly in lymphocytes. G6PDi-1 markedly decreases inflammatory cytokine production in T cells.

For research use only. We do not sell to patients.

G6PDi-1 Chemical Structure

CAS No. : 2457232-14-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 560	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE G6PDi-1

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

G6PDi-1 (10 μM, 2 h) increases the NADP⁺/NADPH ratio in mouse CD8+ and CD4+ T cells^[1].
G6PDi-1 (50 μM, 0-300 min) inhibits mouse and human neutrophil oxidative burst^[1].
G6PDi-1 inhibits the activity of G6PDH in lysates of cultured astrocytes with an IC₅₀ of 102 nM, and lowers the total cellular WST1 reduction (0-100 μM, 60 min)^[2].
G6PDi-1 (0-100 μM,) in combination with CB-839 (0-48 nM) shows synergistic cytotoxicity against A549 (KEAP1 mutant) cells and KPK (Keap1 KO) cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

284.34

Formula

C₁₄H₁₂N₄OS

CAS No.

2457232-14-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

N#CC1=CC(NC2=NC=C(C(CCCC3)=O)C3=N2)=CS1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (175.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5169 mL	17.5846 mL	35.1692 mL
5 mM	0.7034 mL	3.5169 mL	7.0338 mL
10 mM	0.3517 mL	1.7585 mL	3.5169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.79 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (271 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Jonathan M Ghergurovich, et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol. 2020 Jul;16(7):731-739. [Content Brief]

[2]. Watermann P, et al. G6PDi-1 is a Potent Inhibitor of G6PDH and of Pentose Phosphate pathway-dependent Metabolic Processes in Cultured Primary Astrocytes. Neurochem Res. 2023 Oct;48(10):3177-3189. [Content Brief]

[3]. Ding H, et al. Activation of the NRF2 antioxidant program sensitizes tumors to G6PD inhibition. Sci Adv. 2021 Nov 19;7(47):eabk1023. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5169 mL	17.5846 mL	35.1692 mL	87.9229 mL
	5 mM	0.7034 mL	3.5169 mL	7.0338 mL	17.5846 mL
	10 mM	0.3517 mL	1.7585 mL	3.5169 mL	8.7923 mL
	15 mM	0.2345 mL	1.1723 mL	2.3446 mL	5.8615 mL
	20 mM	0.1758 mL	0.8792 mL	1.7585 mL	4.3961 mL
	25 mM	0.1407 mL	0.7034 mL	1.4068 mL	3.5169 mL
	30 mM	0.1172 mL	0.5862 mL	1.1723 mL	2.9308 mL
	40 mM	0.0879 mL	0.4396 mL	0.8792 mL	2.1981 mL
	50 mM	0.0703 mL	0.3517 mL	0.7034 mL	1.7585 mL
	60 mM	0.0586 mL	0.2931 mL	0.5862 mL	1.4654 mL
	80 mM	0.0440 mL	0.2198 mL	0.4396 mL	1.0990 mL
	100 mM	0.0352 mL	0.1758 mL	0.3517 mL	0.8792 mL

G6PDi-1 Related Classifications

Metabolic Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G6PDi-12457232-14-1PDIProtein Disulfide IsomerasesReversiblenon-competitiveglucose-6-phosphate dehydrogenaseG6PDNADPHlymphocytesinflammatory cytokineT cellsInhibitorinhibitorinhibit

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G6PDi-1

Customer Review

G6PDi-1 Related Antibodies

2 Publications Citing Use of MCE G6PDi-1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

G6PDi-1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

G6PDi-1 Related Classifications

Keywords:

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Customer Review

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SAFETY DATA SHEET (SDS)

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