2. Apoptosis
  3. MDM-2/p53
  4. GN25

GN25 is a specific p53-Snail binding inhibitor with antitumor effects.

For research use only. We do not sell to patients.

GN25 Chemical Structure

GN25 Chemical Structure

CAS No. : 1227401-27-5

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Based on 1 publication(s) in Google Scholar

GN25 Related Antibodies

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Description

GN25 is a specific p53-Snail binding inhibitor with antitumor effects[1].

In Vitro

GN25 (10 and 20 μM; 24 h) inhibits cell viability of K-Ras-mutated MEF cells[1].
GN25 (5 μM; 4 h) activates p53 in a K-Ras-dependent manner[1].
GN25 (1-10 μM; 1-6 h) induces p53 and p21 in a dose- and time- dependent manner in K-Ras-mutated A549 and HCT116 cell lines[1].
GN25 activates wild-type p53 in p53WT/MT cells[1].
GN25 (0.25 μM) recovers the masking effect of p53 on CK1/GSK3b-mediated Snail phosphorylation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GN25 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT116, A459 and MKN45 cell lines
Concentration: 10 μM
Incubation Time: 24 h
Result: Obviously suppressed the cell viability of HCT116 and A459 cell lines.

Cell Proliferation Assay[1]

Cell Line: K-Ras-transformed and N-Ras/Myc-transformed MEF cells
Concentration: 10 μM and 20 μM
Incubation Time: 24 h
Result: Inhibited the viability of K-Ras-transformed but not N-Ras/Myc-transformed MEF cells.

Western Blot Analysis[1]

Cell Line: K-Ras-MEF, N-Ras/Myc-MEF and Nontransformed MEF cells
Concentration: 5 μM
Incubation Time: 4 h
Result: Only induced p53 and p21 in K-Ras-MEF cells.

Western Blot Analysis[1]

Cell Line: K-Ras-mutated A549, HCT116 cell lines and MKN45
Concentration: 1, 5, and 10 μM
Incubation Time: 1, 3, and 6 h
Result: Induced p53 and p21 in a dose- and time- dependent manner in A549 and HCT116 cells.
In Vivo

GN25 (10 and 20 mg/kg; i.p. once a week for 10 weeks) blocks the tumor progression and induces tumor regression in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic mice with A549 xenografts[1]
Dosage: 10 and 20 mg/kg
Administration: Intraperitoneal injection; once a week for 10 weeks
Result: Obviously regressed tumors in mice. Showed no significant toxicity in liver, pancreas and kidney.
Molecular Weight

322.33

Formula

C15H14O6S
CAS No.
Appearance

Solid

Color

Light yellow to orange

SMILES

O=C(O)CCSC(C1=O)=CC(C2=C1C(OC)=CC=C2OC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (310.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1024 mL 15.5121 mL 31.0241 mL
5 mM 0.6205 mL 3.1024 mL 6.2048 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1024 mL 15.5121 mL 31.0241 mL 77.5603 mL
5 mM 0.6205 mL 3.1024 mL 6.2048 mL 15.5121 mL
10 mM 0.3102 mL 1.5512 mL 3.1024 mL 7.7560 mL
15 mM 0.2068 mL 1.0341 mL 2.0683 mL 5.1707 mL
20 mM 0.1551 mL 0.7756 mL 1.5512 mL 3.8780 mL
25 mM 0.1241 mL 0.6205 mL 1.2410 mL 3.1024 mL
30 mM 0.1034 mL 0.5171 mL 1.0341 mL 2.5853 mL
40 mM 0.0776 mL 0.3878 mL 0.7756 mL 1.9390 mL
50 mM 0.0620 mL 0.3102 mL 0.6205 mL 1.5512 mL
60 mM 0.0517 mL 0.2585 mL 0.5171 mL 1.2927 mL
80 mM 0.0388 mL 0.1939 mL 0.3878 mL 0.9695 mL
100 mM 0.0310 mL 0.1551 mL 0.3102 mL 0.7756 mL
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GN25 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GN251227401-27-5GN 25GN-25MDM-2/p53antitumorp53HCT116A459MKN45phosphorylationathymic miceInhibitorinhibitorinhibit

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