2. Stem Cell/Wnt
  3. Smo Hedgehog
  4. GSA-10

GSA-10 is a potent smooth (Smo) receptor agonist. GSA-10 is a potent osteogenic molecule. GSA-10 can mediate Hedgehog (Hh) signaling. GSA-10 can be used in regenerative medicine for cancer disease and in the study of fat development.

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GSA-10 Chemical Structure

GSA-10 Chemical Structure

CAS No. : 300833-95-8

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Based on 1 publication(s) in Google Scholar

GSA-10 Related Antibodies

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Description

GSA-10 is a potent smooth (Smo) receptor agonist. GSA-10 is a potent osteogenic molecule. GSA-10 can mediate Hedgehog (Hh) signaling. GSA-10 can be used in regenerative medicine for cancer disease and in the study of fat development[1][2][3].

IC50 & Target

Smoothened receptor[1]
In Vitro

GSA-10 (3 μM; 6 days) can induce cell differentiation into osteoblasts in pluripotent stromal cells C3H10T1/2 with an EC50 of 1.2 μM[1][2].
GSA-10 (10 μM; 24 h) inhibits fat formation in 3T3-L1 preadipocytes by activating the Smo-Lkb1-Ampk pathway[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GSA-10 Related Antibodies

Western Blot Analysis[3]

Cell Line: 3T3-L1 preadipocyte
Concentration: 10 μM
Incubation Time: 24 h
Result: Significantly promoted the phosphorylation of Ampk.
Molecular Weight

450.53

Formula

C26H30N2O5
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(OCCC)C1=CC=C(NC(C2=C(O)C3=C(N(CCCCCC)C2=O)C=CC=C3)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 1.67 mg/mL (3.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2196 mL 11.0980 mL 22.1961 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2196 mL 11.0980 mL 22.1961 mL 55.4902 mL
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GSA-10 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GSA-10300833-95-8GSA10GSA 10SmoHedgehogSmoothenedCell differentiationHuman embryonic cellC3H10T1/23T3-L1Smoothened receptordifferentiationmultipotent mesenchymal progenitor cellcancerregenerative medicineInhibitorinhibitorinhibit

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