H-Ile-Lys-Val-Ala-Val-OH

Name: H-Ile-Lys-Val-Ala-Val-OH
Brand: MedChemExpress
SKU: HY-P4322
Rating: 4.5 (1 reviews)

Cat. No.: HY-P4322 Purity: 99.12%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

H-Ile-Lys-Val-Ala-Val-OH is one of the most potent active sites of laminin-1. H-Ile-Lys-Val-Ala-Val-OH promotes cell adhesion, neurite outgrowth, and tumor growth. H-Ile-Lys-Val-Ala-Val-OH stimulates BMMSC population growth and proliferation by activating MAPK/ERK1/2 and PI3K/Akt signalling pathways.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

H-Ile-Lys-Val-Ala-Val-OH Chemical Structure

CAS No. : 131167-89-0

Size	Price	Stock	Quantity
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE H-Ile-Lys-Val-Ala-Val-OH

•Int J Mol Sci. 2024 May 29;25(11):5961. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

ERK1

ERK2

Akt

In Vitro

H-Ile-Lys-Val-Ala-Val-OH (0-2.5 mM, 0-48 h) induces proliferation and proliferating cell nuclear antigen (PCNA) synthesis of bone marrow mesenchymal stem cell (BMMSC)^[2].
H-Ile-Lys-Val-Ala-Val-OH (5 mM, 24 h) induces the BMMSC cell cycle for cells to enter S phase from G0/G1 and arrests them from entering G2/M phase^[2].
H-Ile-Lys-Val-Ala-Val-OH (0-2.5 mM, 0-48 h) activats MAPK/ERK and PI3K/Akt signalling pathways by enhancing phosphorylation levels of ERK1/2 and Akt in the BMMSCs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	BMMSC
Concentration:	0, 0.004, 0.02, 0.1, 0.5 and 2.5 mM
Incubation Time:	0, 12, 24, 36, 48 h
Result:	Stimulated proliferating cell nuclear antigen (PCNA) synthesis and induced proliferation of bone marrow mesenchymal stem cell (BMMSC) in a dose- and time-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	BMMSC
Concentration:	5 mM
Incubation Time:	24 h
Result:	Induced the BMMSC cell cycle for cells to enter S phase from G0/G1 and arrested them from entering G2/M phase; the increased proportion of S phase in cell cycle was considered to be a sign of BMMSC proliferation.

Western Blot Analysis^[2]

Cell Line:	BMMSC
Concentration:	0, 0.004, 0.02, 0.1, 0.5 and 2.5 mM
Incubation Time:	0, 12, 24, 36, 48 h
Result:	Increased significantly the levels of p-ERK1/2 and p-Akt in a dose- and time-dependent manner.

Molecular Weight

528.69

Formula

C₂₅H₄₈N₆O₆

CAS No.

131167-89-0

Appearance

Solid

Color

White to off-white

Sequence

H-Ile-Lys-Val-Ala-Val-OH

Sequence Shortening

IKVAV

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (189.15 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8915 mL	9.4573 mL	18.9147 mL
5 mM	0.3783 mL	1.8915 mL	3.7829 mL
10 mM	0.1891 mL	0.9457 mL	1.8915 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (246 KB) HNMR (297 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Nomizu M, et al. The all-D-configuration segment containing the IKVAV sequence of laminin A chain has similar activities to the all-L-peptide in vitro and in vivo. J Biol Chem. 1992 Jul 15;267(20):14118-21. [Content Brief]

[2]. Li B, et al. IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation. Cell Prolif. 2014 Apr;47(2):133-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.8915 mL	9.4573 mL	18.9147 mL	47.2867 mL
	5 mM	0.3783 mL	1.8915 mL	3.7829 mL	9.4573 mL
	10 mM	0.1891 mL	0.9457 mL	1.8915 mL	4.7287 mL
	15 mM	0.1261 mL	0.6305 mL	1.2610 mL	3.1524 mL
	20 mM	0.0946 mL	0.4729 mL	0.9457 mL	2.3643 mL
	25 mM	0.0757 mL	0.3783 mL	0.7566 mL	1.8915 mL
	30 mM	0.0630 mL	0.3152 mL	0.6305 mL	1.5762 mL
	40 mM	0.0473 mL	0.2364 mL	0.4729 mL	1.1822 mL
	50 mM	0.0378 mL	0.1891 mL	0.3783 mL	0.9457 mL
	60 mM	0.0315 mL	0.1576 mL	0.3152 mL	0.7881 mL
	80 mM	0.0236 mL	0.1182 mL	0.2364 mL	0.5911 mL
	100 mM	0.0189 mL	0.0946 mL	0.1891 mL	0.4729 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.